Hexokinase-2 antibody
Principal name
Hexokinase-2 antibody
Alternative names for Hexokinase-2 antibody
Hexokinase type II, Muscle form hexokinase, HK2, HKII
Ncbi ID
NP_000180.2, 9606, NP_000180, NM_000189, NP_038848.1, NM_013820, NM_012735
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Hexokinase-2 antibody
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK2 (hexokinase 2) is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this protein is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-348 AI278414.1 4-351 349-732 CB160837.1 11-394 c 733-1006 BM912287.1 1-274 c 1007-1333 AI085541.1 1-327 c 1334-1477 AW134604.1 8-151 1478-4058 AF148513.1 1-2581 4059-5498 BC064369.1 2575-4014 5499-5615 BM706373.1 303-419 5616-7109 BC064369.1 4130-5623
General readings
Furuta H, et al.,(1996) Genomics 36(1):206-209.
Jon E, et al., (2003) J Exp Biology 206:2049-2057.
A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection
Western blot analysis (A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 polyclonal antibody ( Cat # PAB4004 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The HK2 polyclonal antibody ( Cat # PAB4004 ) is used in Western blot to detect HK2 in A-375 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with HK2 polyclonal antibody ( Cat # PAB4005 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The HK2 polyclonal antibody ( Cat # PAB4005 ) is used in Western blot to detect HK2 in A-375 cell lysate.
HK2 monoclonal antibody (M01), clone 4H1. Western Blot analysis of HK2 expression in PC-12(Cat # L012V1 ).
HK2 monoclonal antibody (M01), clone 4H1. Western Blot analysis of HK2 expression in A-431 ( Cat # L015V1 ).
Detection limit for recombinant GST tagged HK2 is approximately 0.3ng/ml as a capture antibody.
Western blot analysis of cell lysate of Jurkat ( 50 µg ) was resolved by SDS - PAGE , transferred to NC membrane and probed with HK2 monoclonal antibody , clone 1A7 ( 1 : 2000 ) ( Cat # MAB1118 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot analysis of HK2 expression in transfected 293T cell line (H00003099-T01) by HK2 MaxPab polyclonal antibody.Lane 1: HK2 transfected lysate(100.87 KDa).Lane 2: Non-transfected lysate.
Hexokinase 2: 12% SDS PAGE
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
The anti-HK2 Pab is used in Western blot to detect HK2 in A375 cell lysate.
Human Skeletal Muscle; Immunohistochemistry with Human Skeletal Muscle lysate tissue at an antibody concentration of 5.0ug/ml using anti-HK2 antibody (ARP54303_P050); HK2 antibody - N-terminal region (ARP54303_P050) in Human Skeletal Muscle cells using Immunohistochemistry
Human HEP3B; . Lanes: 1: 50 ug HEP3B lysate, 2: 50 ug HEP3B lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:1000. Gene Name: HK2. Submitted by: Received from annonymous.; HK2 antibody - N-terminal region (ARP54303_P050) in Human HEP3B cells using Western Blot
Human 721_B; WB Suggested Anti-HK2 Antibody Titration: 1 ug/ml. Positive Control: 721_B cell lysate. HK2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; HK2 antibody - N-terminal region (ARP54303_P050) in Human 721_B cells using Western Blot
Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues (left) and human lung cancer (right) using HK2 antibody Cat.-No AM06497SU-N with DAB staining.
Western blot analysis using HK2 antibody Cat.-No AM06497SU-N against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.
Flow cytometric analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500856, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500856, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500856, Dilution 1:50)
Anti-HK2 mouse monoclonal antibody (TA500856) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HK2(RC209482).
Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody (TA500856).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (RC209482, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.
HEK293T cells transfected with either pCMV6-ENTRY HK2(RC209482)(Red) or empty vector control plasmid(Blue) were immunostained with anti-HK2 mouse monoclonal(TA500856), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (RC209482, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-HK2 monoclonal antibody.(1:200)
HK2 Antibody (E106) (Cat. #TA325029) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HK2 antibody detected the HK2 protein (arrow).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HK2 Antibody (R468) (Cat. #TA325030) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HK2 antibody detected the HK2 protein (arrow).
Western blot analysis of extracts from 293 cells and HepG2 cells, using Smad1 (Phospho-Ser187) antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of U2OS cell using HK2 antibody. Blue: DAPI for nuclear staining.
Lanes: 1: 50 ug HEP3B lysate, 2: 50 ug HEP3B lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1: 1000; Gene Name: HK2; Submitted by: Received from annonymous;
WB Suggested Anti-HK2 Antibody Titration: 1 ug/ml; Positive Control: 721_B cell lysateHK2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Immunohistochemistry with Human Skeletal Muscle lysate tissue at an antibody concentration of 5.0 ug/ml using anti-HK2 antibody
WB Suggested Anti-HK2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HT1080 cell lysateHK2 is supported by BioGPS gene expression data to be expressed in HT1080
Western blot detection of Hexokinase II in COS7,K562 and Hela cell lysates using Hexokinase II mouse mAb (1:1000 diluted).Predicted band size:102KDa.Observed band size:102KDa.
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-5: A431 cells, K562 cells, 231 cells, hela cells, hepG2 cells, Primary antibody: (HK2 Antibody) at dilution 1/1200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (HK2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (HK2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (1)
- Overnight Delivery (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM03129PU-N | Hexokinase-1/2 (1-917) antibody |
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Mouse | IgG1 | 1A7 | Purified | Hu | E, WB |
0.1 ml /
€310.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AM03129PU-S | Hexokinase-1/2 (1-917) antibody |
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Mouse | IgG1 | 1A7 | Purified | Hu | E, WB |
50 µl /
€220.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| TA500856 | Hexokinase-2 antibody |
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| anti-HK2 (Hexokinase II) mouse monoclonal antibody, clone OTI4C5 (formerly 4C5) | |||||||
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Mouse | IgG1 | OTI4C5 | Purified | Can, Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
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| TA325029 | Hexokinase-2 (N-term) antibody |
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| Rabbit polyclonal HK2 (Hexokinase II) Antibody (N-term) | |||||||
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€410.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA325030 | Hexokinase-2 antibody |
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| Rabbit polyclonal HK2 (Hexokinase II) Antibody (Center) | |||||||
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Rabbit | IgG | Purified | Hu | WB |
0.4 ml /
€410.00
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| OriGene Technologies, Inc. | |||||||
| TA326886 | Hexokinase-2 antibody |
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| Rabbit anti-HK2 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA336240 | Hexokinase-2 antibody |
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| Rabbit Polyclonal Anti-HK2 Antibody | |||||||
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Rabbit | IgG | Purified | Bov, Can, Eq, Hu, Ms, Por, Rb, Rt | P, WB |
50 µg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA336241 | Hexokinase-2 antibody |
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| Rabbit Polyclonal Anti-HK2 Antibody | |||||||
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Rabbit | IgG | Purified | Can, Eq, Hu, Ms, Por, Rb, Rt | WB |
50 µg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA346965 | Hexokinase-2 antibody |
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| Mouse Monoclonal Hexokinase II Antibody | |||||||
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Mouse | IgG1 | 3B9-F8-F10 | Purified | WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| TA349617 | Hexokinase-2 antibody |
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| Rabbit Polyclonal Anti-HK2 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA500884 | Hexokinase-2 antibody |
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| anti-HK2 (Hexokinase II) mouse monoclonal antibody, clone OTI4D4 (formerly 4D4) | |||||||
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Mouse | IgG2b | OTI4D4 | Purified | Hu | WB |
0.1 ml /
€350.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AM06497SU-N | Hexokinase-2 antibody |
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Mouse | IgG1 | 3D3 | Ascites | Hu | E, F, P, WB |
0.1 ml /
€400.00
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP15125PU-N | Hexokinase-2 (Center) antibody |
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€370.00
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN209482 | HK2 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN209482BN | HK2 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN209482LP | HK2 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
| KN209482RB | HK2 - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
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| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309482 | Hexokinase-2 |
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| Recombinant protein of human hexokinase 2 (HK2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
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| OriGene Technologies, Inc. | |||||
| AR09039PU-L | Hexokinase-2 (1-917, His-tag) |
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Human | Purified | > 90 % by SDS PAGE | E. coli |
0.5 mg /
€1,500.00
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| OriGene Technologies GmbH | |||||
| +1 additional image |
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| AR09039PU-N | Hexokinase-2 (1-917, His-tag) |
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Human | Purified | > 90 % by SDS PAGE | E. coli |
0.1 mg /
€520.00
|
| OriGene Technologies GmbH | |||||
| +1 additional image |
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