Hexokinase-2 antibody

Principal name

Hexokinase-2 antibody

Alternative names for Hexokinase-2 antibody

Hexokinase type II, Muscle form hexokinase, HK2, HKII

SwissProt ID

A2PYL6 (Horse), O08528 (Mouse), P27881 (Rat), P52789 (Human), Q1W674 (Pig)

Gene ID

3099 (HK2), 15277 (Hk2), 25059

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Chk (Chicken), Ms (Mouse), Por (Porcine), African clawed frog, Can (Canine), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Hexokinase-2 antibody

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK2 (hexokinase 2) is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this protein is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-348 AI278414.1 4-351 349-732 CB160837.1 11-394 c 733-1006 BM912287.1 1-274 c 1007-1333 AI085541.1 1-327 c 1334-1477 AW134604.1 8-151 1478-4058 AF148513.1 1-2581 4059-5498 BC064369.1 2575-4014 5499-5615 BM706373.1 303-419 5616-7109 BC064369.1 4130-5623

General readings

Furuta H, et al.,(1996) Genomics 36(1):206-209.
Jon E, et al., (2003) J Exp Biology 206:2049-2057.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Hexokinase-1/2 (1-917) antibody

A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection Mouse IgG1 1A7 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Hexokinase-1/2 (1-917) antibody

A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection Mouse IgG1 1A7 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

Hexokinase-2 antibody

  anti-HK2 (Hexokinase II) mouse monoclonal antibody, clone OTI4C5 (formerly 4C5)  
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500856, Dilution 1:50) Mouse IgG1 OTI4C5 Purified Can, Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Hexokinase-2 (N-term) antibody

  Rabbit polyclonal HK2 (Hexokinase II) Antibody (N-term)  
HK2 Antibody (E106) (Cat. #TA325029) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HK2 antibody detected the HK2 protein (arrow). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

  Rabbit polyclonal HK2 (Hexokinase II) Antibody (Center)  
HK2 Antibody (R468) (Cat. #TA325030) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HK2 antibody detected the HK2 protein (arrow). Rabbit IgG Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

  Rabbit anti-HK2 Polyclonal Antibody  
Western blot analysis of extracts from 293 cells and HepG2 cells, using Smad1 (Phospho-Ser187) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

  Rabbit Polyclonal Anti-HK2 Antibody  
Lanes: 1: 50 ug HEP3B lysate, 2: 50 ug HEP3B lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1: 1000; Gene Name: HK2; Submitted by: Received from annonymous; Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

  Rabbit Polyclonal Anti-HK2 Antibody  
WB Suggested Anti-HK2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HT1080 cell lysateHK2 is supported by BioGPS gene expression data to be expressed in HT1080 Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

  Mouse Monoclonal Hexokinase II Antibody  
Western blot detection of Hexokinase II in COS7,K562 and Hela cell lysates using Hexokinase II mouse mAb (1:1000 diluted).Predicted band size:102KDa.Observed band size:102KDa. Mouse IgG1 3B9-F8-F10 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

  Rabbit Polyclonal Anti-HK2 Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-5: A431 cells, K562 cells, 231 cells, hela cells, hepG2 cells, Primary antibody: (HK2 Antibody) at dilution 1/1200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

  anti-HK2 (Hexokinase II) mouse monoclonal antibody, clone OTI4D4 (formerly 4D4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (RC209482, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2. Mouse IgG2b OTI4D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Hexokinase-2 antibody

Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues (left) and human lung cancer (right) using HK2 antibody Cat.-No AM06497SU-N with DAB staining. Mouse IgG1 3D3 Ascites Hu E, F, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Hexokinase-2 (Center) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

HK2 - human gene knockout kit via CRISPR

HK2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

HK2 - human gene knockout kit via CRISPR

HK2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

HK2 - human gene knockout kit via CRISPR

HK2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

HK2 - human gene knockout kit via CRISPR

HK2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hk2 - mouse gene knockout kit via CRISPR

Hk2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Hk2 - mouse gene knockout kit via CRISPR

Hk2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hk2 - mouse gene knockout kit via CRISPR

Hk2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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