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Hexokinase-2 antibody

Principal name

Hexokinase-2 antibody

Alternative names for Hexokinase-2 antibody

Hexokinase type II, Muscle form hexokinase, HK2, HKII

SwissProt ID

A2PYL6 (Horse), O08528 (Mouse), P27881 (Rat), P52789 (Human), Q1W674 (Pig)

Gene ID

3099 (HK2), 15277 (Hk2), 25059

Ncbi ID

NP_000180.2, 9606, NP_000180, NM_000189, NP_038848.1, NM_013820, NM_012735

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Chk (Chicken), Ms (Mouse), Por (Porcine), African clawed frog, Can (Canine), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Hexokinase-2 antibody

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK2 (hexokinase 2) is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this protein is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-348 AI278414.1 4-351 349-732 CB160837.1 11-394 c 733-1006 BM912287.1 1-274 c 1007-1333 AI085541.1 1-327 c 1334-1477 AW134604.1 8-151 1478-4058 AF148513.1 1-2581 4059-5498 BC064369.1 2575-4014 5499-5615 BM706373.1 303-419 5616-7109 BC064369.1 4130-5623

General readings

Furuta H, et al.,(1996) Genomics 36(1):206-209.
Jon E, et al., (2003) J Exp Biology 206:2049-2057.

A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection

Western blot analysis (A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 polyclonal antibody ( Cat # PAB4004 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PAB4004

The HK2 polyclonal antibody ( Cat # PAB4004 ) is used in Western blot to detect HK2 in A-375 cell lysate.

PAB4004

Formalin-fixed and paraffin-embedded human cancer tissue reacted with HK2 polyclonal antibody ( Cat # PAB4005 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PAB4005

The HK2 polyclonal antibody ( Cat # PAB4005 ) is used in Western blot to detect HK2 in A-375 cell lysate.

PAB4005

HK2 monoclonal antibody (M01), clone 4H1. Western Blot analysis of HK2 expression in PC-12(Cat # L012V1 ).

H00003099-M01

HK2 monoclonal antibody (M01), clone 4H1. Western Blot analysis of HK2 expression in A-431 ( Cat # L015V1 ).

H00003099-M01

Detection limit for recombinant GST tagged HK2 is approximately 0.3ng/ml as a capture antibody.

H00003099-M01

Western blot analysis of cell lysate of Jurkat ( 50 µg ) was resolved by SDS - PAGE , transferred to NC membrane and probed with HK2 monoclonal antibody , clone 1A7 ( 1 : 2000 ) ( Cat # MAB1118 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.

MAB1118

Western Blot analysis of HK2 expression in transfected 293T cell line (H00003099-T01) by HK2 MaxPab polyclonal antibody.Lane 1: HK2 transfected lysate(100.87 KDa).Lane 2: Non-transfected lysate.

H00003099-B01P

Hexokinase 2: 12% SDS PAGE

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

AP15125PU-N

The anti-HK2 Pab is used in Western blot to detect HK2 in A375 cell lysate.

AP15125PU-N

Human Skeletal Muscle; Immunohistochemistry with Human Skeletal Muscle lysate tissue at an antibody concentration of 5.0ug/ml using anti-HK2 antibody (ARP54303_P050); HK2 antibody - N-terminal region (ARP54303_P050) in Human Skeletal Muscle cells using Immunohistochemistry

ARP54303_P050

Human HEP3B; . Lanes: 1: 50 ug HEP3B lysate, 2: 50 ug HEP3B lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:1000. Gene Name: HK2. Submitted by: Received from annonymous.; HK2 antibody - N-terminal region (ARP54303_P050) in Human HEP3B cells using Western Blot

ARP54303_P050

Human 721_B; WB Suggested Anti-HK2 Antibody Titration: 1 ug/ml. Positive Control: 721_B cell lysate. HK2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; HK2 antibody - N-terminal region (ARP54303_P050) in Human 721_B cells using Western Blot

ARP54303_P050

Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues (left) and human lung cancer (right) using HK2 antibody Cat.-No AM06497SU-N with DAB staining.

AM06497SU-N

Western blot analysis using HK2 antibody Cat.-No AM06497SU-N against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.

AM06497SU-N

Flow cytometric analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).

AM06497SU-N

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500856, Dilution 1:50)

TA500856

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500856, Dilution 1:50)

TA500856

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500856, Dilution 1:50)

TA500856

Anti-HK2 mouse monoclonal antibody (TA500856) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HK2(RC209482).

TA500856

Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody (TA500856).

TA500856

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (RC209482, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.

TA500856

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.

TA500856

HEK293T cells transfected with either pCMV6-ENTRY HK2(RC209482)(Red) or empty vector control plasmid(Blue) were immunostained with anti-HK2 mouse monoclonal(TA500856), and then analyzed by flow cytometry.

TA500856

TA500884

Western blot analysis of extracts (10ug) from 1 cell line by using anti-HK2 monoclonal antibody.(1:200)

TA500884

TP309482

HK2 Antibody (E106) (Cat. #TA325029) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HK2 antibody detected the HK2 protein (arrow).

TA325029

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

TA325029

HK2 Antibody (R468) (Cat. #TA325030) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HK2 antibody detected the HK2 protein (arrow).

TA325030

Western blot analysis of extracts from 293 cells and HepG2 cells, using Smad1 (Phospho-Ser187) antibody.The lane on the right is treated with the synthesized peptide.

TA326886

Immunofluorescence analysis of U2OS cell using HK2 antibody. Blue: DAPI for nuclear staining.

TA326886

Lanes: 1: 50 ug HEP3B lysate, 2: 50 ug HEP3B lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1: 1000; Gene Name: HK2; Submitted by: Received from annonymous;

TA336240

WB Suggested Anti-HK2 Antibody Titration: 1 ug/ml; Positive Control: 721_B cell lysateHK2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

TA336240

Immunohistochemistry with Human Skeletal Muscle lysate tissue at an antibody concentration of 5.0 ug/ml using anti-HK2 antibody

TA336240

WB Suggested Anti-HK2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HT1080 cell lysateHK2 is supported by BioGPS gene expression data to be expressed in HT1080

TA336241

Western blot detection of Hexokinase II in COS7,K562 and Hela cell lysates using Hexokinase II mouse mAb (1:1000 diluted).Predicted band size:102KDa.Observed band size:102KDa.

TA346965

Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-5: A431 cells, K562 cells, 231 cells, hela cells, hepG2 cells, Primary antibody: (HK2 Antibody) at dilution 1/1200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second

TA349617

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (HK2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)

TA349617

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (HK2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)

TA349617

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AM03129PU-N	Hexokinase-1/2 (1-917) antibody
	Mouse	IgG1	1A7	Purified	Hu	E, WB	0.1 ml / €310.00
							Acris Antibodies GmbH
+1 additional image
AM03129PU-S	Hexokinase-1/2 (1-917) antibody
	Mouse	IgG1	1A7	Purified	Hu	E, WB	50 µl / €220.00
							Acris Antibodies GmbH
+1 additional image
TA500856	Hexokinase-2 antibody
	anti-HK2 (Hexokinase II) mouse monoclonal antibody, clone OTI4C5 (formerly 4C5)
	Mouse	IgG1	OTI4C5	Purified	Can, Hu	F, ICC/IF, P, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+7 additional images	Highly validated antibody!
TA325029	Hexokinase-2 (N-term) antibody
	Rabbit polyclonal HK2 (Hexokinase II) Antibody (N-term)
	Rabbit	Ig		Purified	Hu	P, WB	0.4 ml / €410.00
							OriGene Technologies, Inc.
+1 additional image
TA325030	Hexokinase-2 antibody
	Rabbit polyclonal HK2 (Hexokinase II) Antibody (Center)
	Rabbit	IgG		Purified	Hu	WB	0.4 ml / €410.00
							OriGene Technologies, Inc.
TA326886	Hexokinase-2 antibody
	Rabbit anti-HK2 Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt		0.1 mg / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA336240	Hexokinase-2 antibody
	Rabbit Polyclonal Anti-HK2 Antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, Hu, Ms, Por, Rb, Rt	P, WB	50 µg / €360.00
							OriGene Technologies, Inc.
+2 additional images
TA336241	Hexokinase-2 antibody
	Rabbit Polyclonal Anti-HK2 Antibody
	Rabbit	IgG		Purified	Can, Eq, Hu, Ms, Por, Rb, Rt	WB	50 µg / €360.00
							OriGene Technologies, Inc.
TA346965	Hexokinase-2 antibody
	Mouse Monoclonal Hexokinase II Antibody
	Mouse	IgG1	3B9-F8-F10	Purified		WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA349617	Hexokinase-2 antibody
	Rabbit Polyclonal Anti-HK2 Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+2 additional images
TA500884	Hexokinase-2 antibody
	anti-HK2 (Hexokinase II) mouse monoclonal antibody, clone OTI4D4 (formerly 4D4)
	Mouse	IgG2b	OTI4D4	Purified	Hu	WB	0.1 ml / €350.00
							OriGene Technologies, Inc.
+1 additional image
AM06497SU-N	Hexokinase-2 antibody
	Mouse	IgG1	3D3	Ascites	Hu	E, F, P, WB	0.1 ml / €400.00
							Acris Antibodies GmbH
+2 additional images
AP15125PU-N	Hexokinase-2 (Center) antibody
	Rabbit	Ig		Purified	Hu	P, WB	0.4 ml / €370.00
							Acris Antibodies GmbH
+1 additional image

CRISPR

Catalog No.	Species	Donor Vector
KN209482	HK2 - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN209482BN	HK2 - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN209482LP	HK2 - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN209482RB	HK2 - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN307802	Hk2 - mouse gene knockout kit via CRISPR
	Mouse	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN307802BN	Hk2 - mouse gene knockout kit via CRISPR
	Mouse	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN307802LP	Hk2 - mouse gene knockout kit via CRISPR
	Mouse	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.

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