Hexokinase-2 antibody
Principal name
Hexokinase-2 antibody
Alternative names for Hexokinase-2 antibody
Hexokinase type II, Muscle form hexokinase, HK2, HKII
Gene ID
3099 (HK2)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunoprecipitation (IP), Frozen Sections (C)
Background of Hexokinase-2 antibody
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK2 (hexokinase 2) is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this protein is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-348 AI278414.1 4-351 349-732 CB160837.1 11-394 c 733-1006 BM912287.1 1-274 c 1007-1333 AI085541.1 1-327 c 1334-1477 AW134604.1 8-151 1478-4058 AF148513.1 1-2581 4059-5498 BC064369.1 2575-4014 5499-5615 BM706373.1 303-419 5616-7109 BC064369.1 4130-5623
General readings
Furuta H, et al.,(1996) Genomics 36(1):206-209.
Jon E, et al., (2003) J Exp Biology 206:2049-2057.
A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection
Western blot analysis (A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
The anti-HK2 Pab is used in Western blot to detect HK2 in A375 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
The anti-HK2 Pab is used in Western blot to detect HK2 in A375 cell lysate.
Hexokinase 2: 12% SDS PAGE
Human Skeletal Muscle; Immunohistochemistry with Human Skeletal Muscle lysate tissue at an antibody concentration of 5.0ug/ml using anti-HK2 antibody (ARP54303_P050); HK2 antibody - N-terminal region (ARP54303_P050) in Human Skeletal Muscle cells using Immunohistochemistry
Human HEP3B; . Lanes: 1: 50 ug HEP3B lysate, 2: 50 ug HEP3B lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:1000. Gene Name: HK2. Submitted by: Received from annonymous.; HK2 antibody - N-terminal region (ARP54303_P050) in Human HEP3B cells using Western Blot
Human 721_B; WB Suggested Anti-HK2 Antibody Titration: 1 ug/ml. Positive Control: 721_B cell lysate. HK2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; HK2 antibody - N-terminal region (ARP54303_P050) in Human 721_B cells using Western Blot
Hexokinase II antibody detects Hexokinase II protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Hexokinase II antibody (GTX111525) dilution: 1:500.
Hexokinase II antibody detects HK2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Hexokinase II antibody (GTX111525) dilution: 1:500.
Hexokinase II antibody detects HK2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.Green: HK2 protein stained by Hexokinase II antibody (GTX111525) diluted at 1:500.Blue: Hoechst 33342 staining.
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE GTX111525 diluted at 1:10000
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX111525 diluted at 1:10000
Western Blot analysis of HK2 expression in transfected 293T cell line (H00003099-T01) by HK2 MaxPab polyclonal antibody.Lane 1: HK2 transfected lysate(100.87 KDa).Lane 2: Non-transfected lysate.
HK2 monoclonal antibody (M01), clone 4H1. Western Blot analysis of HK2 expression in PC-12(Cat # L012V1 ).
HK2 monoclonal antibody (M01), clone 4H1. Western Blot analysis of HK2 expression in A-431 ( Cat # L015V1 ).
Detection limit for recombinant GST tagged HK2 is approximately 0.3ng/ml as a capture antibody.
Western blot analysis of cell lysate of Jurkat ( 50 µg ) was resolved by SDS - PAGE , transferred to NC membrane and probed with HK2 monoclonal antibody , clone 1A7 ( 1 : 2000 ) ( Cat # MAB1118 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: Hexokinase II Protein [NBC1-18389] - 12% SDS-PAGE (3ug)
SDS-Page: Hexokinase II Protein [NBC1-18389] - Hexokinase 2, 104.1kDa (937aa) with a purity of 95% by SDS - PAGE
Western Blot: Hexokinase II Lysate [NBL1-11579] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for HK2
Immunohistochemistry-Paraffin: Hexokinase II Antibody (3D3) [NBP1-51643] - Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb with DAB staining.
Western Blot: Hexokinase II Antibody (3D3) [NBP1-51643] - Western blot analysis using HK2 mouse mAb against Jurket (1), Hela (2) and HEK293 (3) cell lysate.
Flow cytometry / FACS analysis: Hexokinase II Antibody (3D3) [NBP1-51643] - Flow cytometric analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 polyclonal antibody ( Cat # PAB4004 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The HK2 polyclonal antibody ( Cat # PAB4004 ) is used in Western blot to detect HK2 in A-375 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with HK2 polyclonal antibody ( Cat # PAB4005 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The HK2 polyclonal antibody ( Cat # PAB4005 ) is used in Western blot to detect HK2 in A-375 cell lysate.
Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues (left) and human lung cancer (right) using HK2 antibody Cat.-No AM06497SU-N with DAB staining.
Western blot analysis using HK2 antibody Cat.-No AM06497SU-N against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.
Flow cytometric analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).
Immunohistochemical of paraffin-embedded human skeletal muscle using 22029-1-AP(HK2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using 22029-1-AP(HK2 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using 22029-1-AP(HK2 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using 22029-1-AP(HK2 antibody) at dilution of 1:50 (under 10x lens)
HeLa cells were subjected to SDS PAGE followed by western blot with 22029-1-AP(HK2 antibody) at dilution of 1:1000
Jurkat cells were subjected to SDS PAGE followed by western blot with 22029-1-AP(HK2 antibody) at dilution of 1:1000
A431 cells were subjected to SDS PAGE followed by western blot with 22029-1-AP(HK2 antibody) at dilution of 1:1000
MCF7 cells were subjected to SDS PAGE followed by western blot with 22029-1-AP(HK2 antibody) at dilution of 1:1000
K-562 cells were subjected to SDS PAGE followed by western blot with 22029-1-AP(HK2 antibody) at dilution of 1:2000
Jurkat cells were subjected to SDS PAGE followed by western blot with 22029-1-AP(HK2 antibody) at dilution of 1:1000
HeLa cells were subjected to SDS PAGE followed by western blot with 22029-1-AP(HK2 antibody) at dilution of 1:1000
IP Result of anti-HK2 (IP:22029-1-AP, 3ug; Detection:22029-1-AP 1:1000) with HeLa cells lysate 2000ug.
Rabbit anti-Hexokinase-2 Antibody Cat.-No AP32497PU-N. The Type I isozyme, purified from Rat brain (Wilson, J.E., Prep. Biochem. 19, 13-21, 1989), and the rat Type II and Type III isozymes, expressed in yeast (Sui, D., and Wilson, J.E., Prot. Exp. Purif. 24, 83-89, 2002), were used for SDS gel electrophoresis and immunoblotting. The top panel shows the gel after staining with Coomassie Blue (5 μg protein in each lane). The bottom three panels show replicate immunoblots probed with a monoclonal to Hexokinase I (Cat.-No AM32570SU-N), affinity-purified polyclonal Rabbit antiserum against Hexokinase-2 Antibody (Cat.-No AP32497PU-N), or a monoclonal to Hexokinase III (Cat.-No AM32571SU-N).
A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC
Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb (GTX82791) with DAB staining.
Western blot analysis using HK2 mouse mAb (GTX82791) against Jurkat (1), Hela (2) and HEK293 (3) cell lysate
Flow cytometric analysis of K562 cells using HK2 mouse mAb (GTX82791, green) and negative control (purple)
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-HK2mouse monoclonal antibody. (GTX84357, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-HK2mouse monoclonal antibody. (GTX84357, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-HK2mouse monoclonal antibody. (GTX84357, Dilution 1:50)
Anti-HK2 mouse monoclonal antibody (GTX84357) immunofluorescent staining of COS7 cells transiently transfected with HK2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.
HEK293T cells transfected with either pCMV6-ENTRY HK2
Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody (GTX84357).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500856, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500856, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500856, Dilution 1:50)
Anti-HK2 mouse monoclonal antibody (TA500856) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HK2(RC209482).
Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody (TA500856).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (RC209482, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.
HEK293T cells transfected with either pCMV6-ENTRY HK2(RC209482)(Red) or empty vector control plasmid(Blue) were immunostained with anti-HK2 mouse monoclonal(TA500856), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (RC209482, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-HK2 monoclonal antibody.(1:200)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM03129PU-N | Hexokinase-1/2 (1-917) antibody |
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Mouse | IgG1 | 1A7 | Purified | Hu | E, WB |
0.1 ml /
€310.00
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| Acris Antibodies GmbH | |||||||
 +1 additional image |
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| AM03129PU-S | Hexokinase-1/2 (1-917) antibody |
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|
Mouse | IgG1 | 1A7 | Purified | Hu | E, WB |
50 µl /
€220.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| TA500856 | Hexokinase-2 antibody |
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 |
Mouse | IgG1 | OTI4C5 | Purified | Can, Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
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| TA325029 | Hexokinase-2 (N-term) antibody |
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Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA325030 | Hexokinase-2 antibody |
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 |
Rabbit | IgG | Purified | Hu | WB |
0.4 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA326886 | Hexokinase-2 antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA336240 | Hexokinase-2 antibody |
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 |
Rabbit | IgG | Purified | Bov, Can, Eq, Hu, Ms, Por, Rb, Rt | P, WB |
50 µg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA336241 | Hexokinase-2 antibody |
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 |
Rabbit | IgG | Purified | Can, Eq, Hu, Ms, Por, Rb, Rt | WB |
50 µg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA346965 | Hexokinase-2 antibody |
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 |
Mouse | IgG1 | 3B9-F8-F10 | Purified | WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA500884 | Hexokinase-2 antibody |
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 |
Mouse | IgG2b | OTI4D4 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| AM06497SU-N | Hexokinase-2 antibody |
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 |
Mouse | IgG1 | 3D3 | Ascites | Hu | E, F, P, WB |
0.1 ml /
€400.00
|
| Acris Antibodies GmbH | |||||||
| +2 additional images |
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| AP15125PU-N | Hexokinase-2 (Center) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
Regular Price: 0.4 ml / €370.00 Special Price: 0.4 ml / €231.00 |
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| H00003099-B01P | Hexokinase-2 antibody |
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Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
|
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| Abnova Taiwan Corp. | |||||||
| H00003099-M01 | Hexokinase-2 antibody |
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 |
Mouse | IgG2a | 4H1 | Purified | Hu, Rt | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +2 additional images |
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| MAB1118 | Hexokinase-2 antibody |
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Mouse | IgG1 | 1A7 | Hu | E, WB |
0.1 ml /
€390.00
|
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| Abnova Taiwan Corp. | |||||||
| PAB4004 | Hexokinase-2 antibody |
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 |
Rabbit | Hu | E, P, WB |
0.4 ml /
€390.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| PAB4005 | Hexokinase-2 antibody |
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Rabbit | Hu | E, P, WB |
0.4 ml /
€390.00
|
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| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| ARP54303_P050 | Hexokinase-2 antibody |
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Rabbit | Aff - Purified | African clawed frog, Bov, Chk, Hu, Ms, Por, Rt | P, WB |
50 µg /
€350.00
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| AVIVA Systems Biology | |||||||
| +2 additional images |
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| ARP54304-P050 | Hexokinase-2 antibody |
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Rabbit | Aff - Purified | Hu | WB |
50 µg /
€350.00
|
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| AVIVA Systems Biology | |||||||
| NBP1-04296 | Hexokinase-2 antibody |
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Mouse | IgG1 | 1A7 | Purified | Hu | E, WB |
0.1 ml /
€380.00
|
| Novus Biologicals Inc. | |||||||
