Hexokinase-2 antibody

Principal name

Hexokinase-2 antibody

Alternative names for Hexokinase-2 antibody

Hexokinase type II, Muscle form hexokinase, HK2, HKII

SwissProt ID

A2PYL6 (Horse), O08528 (Mouse), P27881 (Rat), P52789 (Human), Q1W674 (Pig)

Gene ID

3099 (HK2)

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Can (Canine), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunoprecipitation (IP), Frozen Sections (C)

Background of Hexokinase-2 antibody

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK2 (hexokinase 2) is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this protein is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-348 AI278414.1 4-351 349-732 CB160837.1 11-394 c 733-1006 BM912287.1 1-274 c 1007-1333 AI085541.1 1-327 c 1334-1477 AW134604.1 8-151 1478-4058 AF148513.1 1-2581 4059-5498 BC064369.1 2575-4014 5499-5615 BM706373.1 303-419 5616-7109 BC064369.1 4130-5623

General readings

Furuta H, et al.,(1996) Genomics 36(1):206-209.
Jon E, et al., (2003) J Exp Biology 206:2049-2057.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Hexokinase-1/2 (1-917) antibody

A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection Mouse IgG1 1A7 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Hexokinase-1/2 (1-917) antibody

A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection Mouse IgG1 1A7 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

Hexokinase-2 antibody

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-HK2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500856, Dilution 1:50) Mouse IgG1 OTI4C5 Purified Can, Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Hexokinase-2 (N-term) antibody

HK2 Antibody (E106) (Cat. #TA325029) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HK2 antibody detected the HK2 protein (arrow). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

HK2 Antibody (R468) (Cat. #TA325030) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HK2 antibody detected the HK2 protein (arrow). Rabbit IgG Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

Western blot analysis of extracts of various cell lines, using HK2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

Lanes: 1: 50 ug HEP3B lysate, 2: 50 ug HEP3B lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1: 1000; Gene Name: HK2; Submitted by: Received from annonymous; Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

WB Suggested Anti-HK2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HT1080 cell lysateHK2 is supported by BioGPS gene expression data to be expressed in HT1080 Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

Western blot detection of Hexokinase II in COS7,K562 and Hela cell lysates using Hexokinase II mouse mAb (1:1000 diluted).Predicted band size:102KDa.Observed band size:102KDa. Mouse IgG1 3B9-F8-F10 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Hexokinase-2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (RC209482, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2. Mouse IgG2b OTI4D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Hexokinase-2 antibody

Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues (left) and human lung cancer (right) using HK2 antibody Cat.-No AM06497SU-N with DAB staining. Mouse IgG1 3D3 Ascites Hu E, F, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Hexokinase-2 (Center) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

Hexokinase-2 antibody

Western Blot analysis of HK2 expression in transfected 293T cell line (H00003099-T01) by HK2 MaxPab polyclonal antibody.

Lane 1: HK2 transfected lysate(100.87 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Hexokinase-2 antibody

HK2 monoclonal antibody (M01), clone 4H1. Western Blot analysis of HK2 expression in PC-12(Cat # L012V1 ). Mouse IgG2a 4H1 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Hexokinase-2 antibody

Western blot analysis of cell lysate of Jurkat ( 50 µg ) was resolved by SDS - PAGE , transferred to NC membrane and probed with HK2 monoclonal antibody , clone 1A7 ( 1 : 2000 ) ( Cat # MAB1118 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 1A7 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Hexokinase-2 antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 polyclonal antibody ( Cat # PAB4004 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Hexokinase-2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with HK2 polyclonal antibody ( Cat # PAB4005 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Hexokinase-2 antibody

Human Skeletal Muscle; Immunohistochemistry with Human Skeletal Muscle lysate tissue at an antibody concentration of 5.0ug/ml using anti-HK2 antibody (ARP54303_P050); HK2 antibody - N-terminal region (ARP54303_P050) in Human Skeletal Muscle cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Por, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

Hexokinase-2 antibody

Hexokinase-2 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Hexokinase-2 antibody

Hexokinase-2 Mouse IgG1 1A7 Purified Hu E, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

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