Hexokinase-3 antibody

Principal name

Hexokinase-3 antibody

Alternative names for Hexokinase-3 antibody

HK3, Hexokinase type III, HKIII

SwissProt ID

P27926 (Rat), P52790 (Human), Q3TRM8 (Mouse)

Gene ID

3101 (HK3), 531017 (AHSA2)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Functional assay (FN), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Hexokinase-3 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

General readings

Jon E. et al., (2003) J.Exp Biology 206:2049-2057. 
Furuta H. et al.,(1996) Genomics 36(1):206-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Hexokinase-3 antibody

Western blot analysis of extracts from Jurkat cells, treated with insulin (0.01U/ml, 15mins), using Hexokinase-3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Hexokinase-3 antibody

Western blot analysis: (A): Recombinant protein (20ng or 100ng) of Hexokinase four isoforms were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase 3 (1:1000). (B): Cell lysates (30 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 4F5 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Hexokinase-3 antibody

Western blot analysis: (A): Recombinant protein (20ng or 100ng) of Hexokinase four isoforms were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase 3 (1:1000). (B): Cell lysates (30 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 4F5 Purified Hu E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

Hexokinase-3 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Hexokinase-3 (N-term) antibody

Western blot analysis of anti-HK3 Pab in Hela cell line lysate (35ug/lane). HK3(arrow) was detected using the purified Pab (1:40 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Hexokinase-3 antibody

Western blot (WB) analysis of HXK III antibody (Cat.-No.: AP20526PU-N) in extracts from Jurkat insulin 0.01U cells. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Hexokinase-3 antibody

Hexokinase-3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Hexokinase-3 antibody

HK3 MaxPab polyclonal antibody. Western Blot analysis of HK3 expression in human spleen. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Hexokinase-3 antibody

Western Blot analysis of HK3 expression in transfected 293T cell line (H00003101-T02) by HK3 MaxPab polyclonal antibody.

Lane 1: HK3 transfected lysate(99.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Hexokinase-3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with HK3 polyclonal antibody ( Cat # PAB4001 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Hexokinase-3 antibody

Western blot analysis of HK3 polyclonal antibody ( Cat # PAB4002 ) in Hela cell lysate ( 35 µg/lane ) . HK3 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 40 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Hexokinase-3 antibody

Western blot analysis of extracts from Jurkat cells, treated with insulin 0.01U/ml 15', using Hexokinase-3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

Hexokinase-3 antibody

Western blot analysis of extracts from Jurkat cells, treated with insulin 0.01U/ml 15', using Hexokinase-3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

Hexokinase-3 antibody

Immunohistochemical analysis of Hexokinase 3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Hexokinase-3 antibody

Immunohistochemical analysis of Hexokinase 3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Hexokinase-3 antibody

Immunohistochemical analysis of Hexokinase 3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Hexokinase-3 antibody

Hexokinase-3 Rabbit Aff - Purified Hu WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Hexokinase-3 antibody

Hexokinase-3 Mouse IgG1 4F5 Purified Hu E, SDS-PAGE, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Hexokinase-3 antibody

Hexokinase-3 Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Hexokinase-3 antibody

Hexokinase-3 Rabbit IgG Aff - Purified Hu E, WB
50 µg / €290.00
  Novus Biologicals Inc.

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