HGF receptor antibody

Principal name

HGF receptor antibody

Alternative names for HGF receptor antibody

Hepatocyte growth factor receptor, MET, Scatter factor receptor, HGF/SF receptor, c-Met, Met proto-oncogene tyrosine kinase

SwissProt ID

P08581 (Human), P16056 (Mouse), P97523 (Rat), Q2QLE0 (Pig), Q769I5 (Bovin)

Gene ID

4233 (MET), 24553 (Met)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit), Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Neutralisation (NEUT), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunoprecipitation (IP)

Background of HGF receptor antibody

Hepatocyte growth factor receptor (HGF R), a product of the proto-oncogene c-Met, is a heterodimeric transmembrane glycoprotein that is a receptor-type tyrosine kinase. The c-Met heterodimer is composed of an alpha chain that is disulfide-linked to a beta chain. Each alpha and beta subunit heterodimer contain 1152 amino acid residues with a calculated molecular mass of approximately 129 kDa. The alpha chain is exposed to the cell surface and the beta chain spans the plasma membrane. c-Met is synthesized as a single-chain precursor which undergoes cotranslational glycosylation and proteolytic cleavage producing the heterodimeric mature form. Human and mouse HGF receptors share 89% amino acid identity. HGF is the ligand for the HGF receptor. Human HGF can bind to the mouse HGF receptor.Hepatocyte growth factor (HGF), also known as scatter factor (SF), is a multifunctional cytokine that promotes mitogenesis, migration, invasion, and morphogenesis. HGF stimulates hepatocytes and other epithelial and endothelial cells by various biological actions. HGF binding involves the beta chain of the HGF receptor, but alpha chain participation cannot be ruled out. HGF binding to c-Met triggers dimerization and subsequent tyrosine autophosphorylation of the receptor beta chain. Autophophorylation at two tyrosines upregulates kinase activity while phosphorylation at two other tyrosines generates SH2 docking sites for adapter proteins such as Shc, Grb2, CrK/CRKL, and Gab1. Receptor activation has been correlated to the activation of the Ras pathway, which culminates in the activation and consequent nuclear translocation of MAP kinase. c-Met can also be negatively modulated by phosphorylation of Ser 985 by protein kinase C. Other ligand-receptor activities involve binding that leads to enhanced integrin-mediated B cell and lymphoma cell adhesion. Normal HGF-Met signaling is needed for embryonic development and abnormal signaling and has been implicated in tumorigenesis.

General readings

Fan S et al. The multisubstrate adapter Gab1 regulates hepatocyte growth factor (scatter factor)-c-Met signaling for cell survival and DNA repair. Mol Cell Biol 21:4968-84 (2001). PubMed PMID: 11438654
Crostella L et al. Hepatocyte Growth Factor/scatter factor-induces phosphorylation of cortactin in A431 cells in a Src kinase-independent manner. Oncogene 20:3735-45 (2001). PubMed PMID: 11439336
Parr C et al. The HGF/SF-induced phosphorylation of paxillin, matrix adhesion, and invasion of prostate cancer cells were suppressed by NK4, an HGF/SF variant. Biochem Biophys Res Commun 285:1330-7 (2001). PubMed PMID: 11478803
Longati P et al. Receptor tyrosine kinases as therapeutic targets: the model of the MET oncogene. Curr Drug Targets 2:41-55 (2001). PubMed PMID: 11465538
Giordano S et al. Biosynthesis of the protein encoded by the c-met proto-oncogene. Oncogene 4:1383-8 (1989). PubMed PMID: 2554238

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HGF receptor antibody

Immunohistochemical staining of paraffin-embedded MET positive Human lung carcinoma tissue using anti-MET mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110C for 10 min, UM800082)(1:150) Mouse IgG1 UMAB190 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

HGF receptor antibody

Immunohistochemical staining of paraffin-embedded MET positive Human lung carcinoma tissue using anti-MET mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110C for 10 min, UM800082)(1:150) Mouse IgG1 UMAB190 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

HGF receptor (catalytic domain) antibody

Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent Ã?s different anti-Met monoclonal antibodies that are Cat# AM1001-1005. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# AM1000). Mouse IgG1 4AT44 Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HGF receptor non pTyr1003 antibody

Human breast carcinoma stained with anti-c-Met  antibody (Paired1003) Rabbit SP59 Purified Hu P
0.5 ml / €520.00
  Acris Antibodies GmbH

HGF receptor non pTyr1003 antibody

Human breast carcinoma stained with anti-c-Met  antibody (Paired1003) Rabbit SP59 Purified Hu P
1 ml / €750.00
  Acris Antibodies GmbH

HGF receptor non pThr1003 antibody

Human breast carcinoma stained with anti-c-Met  antibody (Paired1003) Rabbit SP59 Purified Hu P
0.1 ml / €290.00
  Acris Antibodies GmbH

HGF receptor (C-term) antibody

Human ovarian ca. stained with anti-c-Met, AP15492PU. Rabbit Aff - Purified Hu P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

HGF receptor (C-term) antibody

Human ovarian ca. stained with anti-c-Met, AP15492PU. Rabbit Purified Hu P, WB
1 ml / €520.00
  Acris Antibodies GmbH

HGF receptor (C-term) antibody

Human ovarian ca. stained with anti-c-Met, AP15492PU. Rabbit Purified Hu P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

HGF receptor pTyr1349/1356 antibody

Western blot using affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary B5/589 epithelial cells were serum-deprived and treated with or without HGF. Cell lysates were immunoprecipated with the anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane, and probed with anti-c-Met pY1349pY1356. Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HGF receptor (catalytic domain) antibody

Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent Ã?s different anti-Met monoclonal antibodies that are Cat# AM1001-1005. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# AM1000). Mouse IgG1 6AT203 Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HGF receptor (catalytic domain) antibody

Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent 聮s different anti-Met monoclonal antibodies that are Cat# AM1001-1005. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# AM1000). Mouse IgG1 6AT1785 Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HGF receptor (catalytic domain) antibody

Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent Ã?s different anti-Met monoclonal antibodies that are Cat# AM1001-1005. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# AM1000). Mouse IgG1 4AT259 Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HGF receptor (catalytic domain) antibody

Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent Ã?s different anti-Met monoclonal antibodies that are Cat# AM1001-1005. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# AM1000). Mouse IgG1 4AT247 Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HGF receptor antibody

HGF receptor Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HGF receptor antibody

Western blot analysis of Met expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HGF receptor antibody

Western blot analysis of Met expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HGF receptor antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MET (RC217003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MET. Mouse IgG2b OTI2B5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HGF receptor antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MET (RC217003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MET. Mouse IgG1 OTI2H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HGF receptor antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MET (RC217003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MET. Mouse IgG2a OTI18A5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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