HIF1 beta / ARNT antibody

Principal name

HIF1 beta / ARNT antibody

Alternative names for HIF1 beta / ARNT antibody

BHLHE2, Dioxin receptor, Hypoxia-inducible factor 1 beta, HIF-1 beta, Aryl hydrocarbon receptor nuclear translocator, Class E basic helix-loop-helix protein 2

SwissProt ID

P27540 (Human), P41739 (Rat), P53762 (Mouse), Q9BE97 (Bovin)

Gene ID

405 (ARNT), 25242 (Arnt)

Available reactivities

Bov (Bovine), Ft (Ferret), Hu (Human), Ms (Mouse), Rt (Rat), Sh (Sheep), GP (Guinea Pig), Mky (Monkey), Por (Porcine), Rb (Rabbit), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

ChIP assay (ChIP), Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Electrophoretic Mobility Shift Assay (EMSA), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Flow Cytometry (F), Luminex (LMNX)

Background of HIF1 beta / ARNT antibody

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia. The HIF1 complex consists of two subunits, HIF-1a and HIF-1ß, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1a is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1a is proline hydroxylated leading to a conformatiol change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitition and proteasomal degradation follows. Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1a degradation and lead to its stabilization. In addition, HIF-1a can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway.HIF-1ß is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex. Together with AhR, HIF-1ß plays an important role in xenobiotics metabolism. In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia. Studies also found that ARNT/HIF-1ß expression levels decrease significantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1ß plays an important role in pancreatic ß-cell function.

General readings

1. Fukuda R, Zhang H, Kim JW, Shimoda L, Dang CV, Semenza GL. Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
2. Creighton-Gutteridge M, Cardellina JH 2nd, Stephen AG, Rapisarda A, Uranchimeg B, Hite K, Denny WA, Shoemaker RH, Melillo G. Screening Technologies Branch, Developmental Therapeutics Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, Maryland 21702, USA.
See individual datasheets.
1. Khan, Aahida., et al. Gastrointestinal, gepatobiliary and pancreatic pathology: Peroxisomal Localization of Hypoxia-Inducible Factors and Hypoxia Inducible Factor Regulatory Hydroxylases in Primary Rat Hepatocytes Exposed to Hypoxia-Reoxygenation. 169: 1251-1269, 2006.
2. Creighton-Gutteridge, M., et al. Cell Type-Specific Topoisomerase II-Dependent Inhibition of Hypoxia-Inducible Factor-1alpha Protein Accumulation by NSC 644221. Clin. Cancer Res. 2007 13: 1010-1018.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HIF1 beta / ARNT antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501147) Mouse IgG1 OTI1A1 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

HIF1 beta / ARNT antibody

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501148, Dilution 1:50) Mouse IgG1 OTI1D10 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF1 beta / ARNT antibody

Anti-ARNT mouse monoclonal antibody (TA501150) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNT(RC216724). Mouse IgG1 OTI1D1 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF1 beta / ARNT antibody

Immunofluorescent staining of HeLa cells using anti-ARNT mouse monoclonal antibody (TA501249). Mouse IgG1 OTI2C7 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF1 beta / ARNT antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3D10 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

HIF1 beta / ARNT antibody

Immunofluorescence of purified MaxPab antibody to ARNT on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

HIF1 beta / ARNT antibody

Immunoperoxidase of monoclonal antibody to ARNT on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml] Mouse IgG2a 3D10 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HIF1 beta / ARNT antibody

Western Blot: HIF-1 beta Antibody TA301442 - WB analysis of HIF-1 beta in MCF7 whole cell lysate. Rabbit IgG Purified Bov, Ft, Hu, Ms, Rt, Sh WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1 beta / ARNT antibody

Western Blot: HIF-1 beta Antibody (H1beta234)TA301443 - Analysis of HIF-1 beta in HeLa nuclear extract. Mouse IgG1 H1beta234 Purified Bov, Ft, Hu, Ms, Rt, Sh WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1 beta / ARNT antibody

Western blot analysis of extracts from HepG2 cells, using ARNT antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1 beta / ARNT antibody

Predicted band size: 87 kDa . Positive control: Hepg2, MCF7, NIH3T3, U20S and Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Hepg2 cell lysate Lane2: MCF7 cell lysate Lane3: NIH3T3 cell lysate Lane4: U20S cell lysate Lane5: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1 beta / ARNT antibody

ARNT Antibody (V528) (Cat. #TA324545) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ARNT antibody detected the ARNT protein (arrow). Rabbit IgG Purified Bov, Hu ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HIF1 beta / ARNT (558-570) antibody

TA326733 (1µg/ml) staining of HeLa nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF1 beta / ARNT (69-82) antibody

TA326734 (2µg/ml) staining of HepG2 nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF1 beta / ARNT antibody

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501149, Dilution 1:50) Mouse IgG1 OTI1F4 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF1 beta / ARNT antibody

ELISA: HIF-1 beta Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

HIF1 beta / ARNT antibody

ARNT Luminex with 1D10 Capture (TA600042) and 1A1 Detection (TA700042) Antibodies. Substrate used: Recombinant Human ARNT (TP316724) Mouse IgG1 OTI1D10 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF1 beta / ARNT antibody

ARNT Luminex with 1D10 Capture (TA600042) and 1A1 Detection (TA700042) Antibodies. Substrate used: Recombinant Human ARNT (TP316724) Mouse IgG1 OTI1A1 Purified Hu LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

HIF1 beta / ARNT antibody

ARNT Luminex with 1D10 Capture (TA600042) and 2C7 Detection (TA700046) Antibodies. Substrate used: Recombinant Human ARNT (TP316724) Mouse IgG1 OTI2C7 Purified Hu LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

HIF1 beta / ARNT (528-544) antibody

Figure 1. Staining HIF-1 Beta in Kidney by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

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