HIF1A / HIF1 alpha antibody

Principal name

HIF1A / HIF1 alpha antibody

Alternative names for HIF1A / HIF1 alpha antibody

HIF-1 alpha, Hypoxia-inducible factor 1 alpha, ARNT-interacting protein, Member of PAS protein 1, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78

SwissProt ID

O35800 (Rat), Q16665 (Human), Q61221 (Mouse), Q8R385 (Mouse), Q9I8A9 (Xenla), Q9XTA5 (Bovin), Q9YIB9 (Chick)

Gene ID

3091 (HIF1A), 15251 (Hif1a), 29560

Available reactivities

Hu (Human), Ms (Mouse), Mink, Rt (Rat), Sh (Sheep), Bov (Bovine), Chk (Chicken), Rb (Rabbit), Mky (Monkey), Prim (Primate), Can (Canine), Por (Porcine), Ft (Ferret), Xen (Xenopus), Hst (Hamster), Gt (Goat), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Goat

Available applications

Electrophoretic Mobility Shift Assay (EMSA), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), ChIP assay (ChIP), Flow Cytometry (F)

Background of HIF1A / HIF1 alpha antibody

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformatiol change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitition and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8). In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia (9). Studies also found that ARNT/HIF-1β expression levels decrease significantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1β plays an important role in pancreatic β-cell function (10).

General readings

Okuyama H. et al., (2006) J. Biol Chem. 281(22):15554-63. Berra E. et al., (2006) EMBO.7(1):41-5.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HIF1A / HIF1 alpha (329-530) antibody

  Mouse Monoclonal Anti-HIF1 alpha Antibody  
Western blot analysis of HIF1 Alpha in HeLa cell lysates using a 1:500 dilution of the antibody Mouse IgG1 ESEE122 Purified Bov, Hu, Ms, Rt C, E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Mouse Monoclonal HIF-1 alpha Antibody (ESEE122)  
Immunofluorescence: HIF-1 alpha Antibody (ESEE122) - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using anti-HIF1A. 100X magnification. Blue dye is DAPI nuclear staining. Photos courtesy of Susan Alexander and Hattie Gresham, PhD. Mouse IgG1 ESEE122 Purified Bov, Can, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67)  
Western Blot: HIF-1 alpha Antibody (H1alpha67) - Upon quantification, 100ug of MEF cells (+/+,-/-) protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at 1:500. The secondary antibody was conjugated with HRP and was used at  1:2500. Mouse IgG2b H1alpha67 Purified Bov, Ft, Hu, Ms, Por, Prim, Rt, Sh IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit Polyclonal Anti-HIF1A Antibody  
HIF1A antibody - middle region validated by WB using chicken liver Rabbit IgG Purified Bov, Can, Gt, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit Polyclonal HIF-1 alpha Antibody  
Western Blot: HIF-1 alpha Antibody [TA336260] - Analysis of TA336260, anti-HIF-1 alpha, on normoxic and hypoxic nuclear rat cell lysates. Rabbit Purified Can, GP, Hu, Ms, Prim, Rt C, ChIP, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit Polyclonal HIF-1 alpha Antibody  
Western Blot: HIF-1 alpha Antibody [TA336308] - Analysis of HIF-1 alpha in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen. Rabbit Purified Gt, Hst, Hu, Ms, Prim, Rb, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and lung cancer tissues (right) using HIF1A antibody Cat.-No AM06608SU-N with DAB staining. Mouse IgG1 1A3 Ascites Hu, Mky, Ms E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (C-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha antibody

  Mouse Monoclonal HIF-1 alpha Antibody (HA111)  
Western Blot: HIF-1 alpha Antibody (HA111) - Westerm blot detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein. (explants were subjected to 21 and 2% oxygen for 4 hours). Mouse IgG2 HA111 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha (Hydroxy P564) antibody

  Rabbit polyclonal Hif-1 alpha hydroxy P564 antibody  
WB using anti-HIF-1alphaHYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining (right panel) using conventional anti-HIF-1alpha. The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO. Rabbit IgG Serum Bov, Can, Hu, Ms, Rt, Xen E, WB
0.1 ml / €356.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit polyclonal HIF1A Antibody (N-term)  
Western blot analysis of HIF1A Antibody (N-term) (Cat. #TA324790) in CHO cell line lysates (35ug/lane). HIF1A (arrow) was detected using the purified Pab. Rabbit IgG Purified Bov, Hst, Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit Polyclonal HIF1A antibody  
Western blot analysis of extract from HUVEC cells using HIF-1aantibody,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit Polyclonal anti-HIF1A antibody  
WB Suggested Anti-HIF1A Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Heart Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit anti-HIF1A Monoclonal Antibody  
Western blot analysis of extracts of A549 cell line, using HIF1A antibody. Mouse IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit Polyclonal HIF-1 alpha Antibody  
Western Blot: HIF-1 alpha Antibody [TA336484] - Detection of HIF-1 alpha in Cobalt Chloride treated/untreated Cos-7 nuclear extracts using TA336484. Rabbit Purified Hu, Ms, Por, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit Polyclonal HIF-1 alpha Antibody  
Western Blot: HIF-1 alpha Antibody [TA336704] - Detection of HIF-1 alpha using TA336704.  Lane 1: COS7 CoCl treated cells  Lane 2: COS7 untreated cells. Rabbit Purified Hu, Ms, Por WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit Polyclonal HIF-1 alpha [Exon 9] Antibody (exon 9)  
Western Blot: HIF-1 alpha [Exon 9] Antibody (exon 9) [TA336763] - Western blot on human recombinant HIF-1 alpha protein. Rabbit exon 9 Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit Polyclonal HIF-1 alpha [Exon 10] Antibody (exon 10)  
Western Blot: HIF-1 alpha [Exon 10] Antibody (exon 10) [TA336764] - Western blot on human recombinant HIF-1 alpha protein Rabbit exon 10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit Polyclonal HIF-1 alpha [Exon 12] Antibody (exon 12)  
Western Blot: HIF-1 alpha [Exon 12] Antibody (exon 12) [TA336765] - Western blot on human recombinant HIF-1 alpha protein Rabbit exon 12 Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

  Rabbit Polyclonal HIF-1 alpha [Exon 13] Antibody (exon 13)  
Western Blot: HIF-1 alpha [exon 13] Antibody [TA336766] - Western blot on human recombinant HIF-1 alpha protein. Rabbit exon 13 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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