HIF1A / HIF1 alpha antibody

Principal name

HIF1A / HIF1 alpha antibody

Alternative names for HIF1A / HIF1 alpha antibody

HIF-1 alpha, Hypoxia-inducible factor 1 alpha, ARNT-interacting protein, Member of PAS protein 1, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78

SwissProt ID

O35800 (Rat), Q16665 (Human), Q61221 (Mouse), Q8R385 (Mouse), Q9I8A9 (Xenla), Q9XTA5 (Bovin), Q9YIB9 (Chick)

Gene ID

15251 (Hif1a), 3091 (HIF1A)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Can (Canine), Chk (Chicken), Eq (Equine), Fi (Fish), Hst (Hamster), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Xen (Xenopus), Tr (Turkey), L (Lizard), Bear, Rb (Rabbit), Ft (Ferret), Sh (Sheep), Mink, GP (Guinea Pig), Gt (Goat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Electrophoretic Mobility Shift Assay (EMSA), ELISA (detection) (E(detection)), ChIP assay (ChIP), Flow Cytometry (F)

Background of HIF1A / HIF1 alpha antibody

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformatiol change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitition and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1β is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1β plays an important role in xenobiotics metabolism (8). In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia (9). Studies also found that ARNT/HIF-1β expression levels decrease significantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1β plays an important role in pancreatic β-cell function (10).

General readings

These references discuss the use of this clone but not this particular antibody production. 1. Talks, K.L. and Turley, H. Amer. J. of Pathology. 157: 411-421 (2000) (Immunohistochemistry and Western Blot) 2. Weisener, M.S. and Turley, H. Blood. 92: 2260-2268 (1998) 3. Giatromanolaki, A., et al. Cancer Research. 61: 7992-7998 (2001) 4. Koukourakis, MI, et al. Cancer Research. 61: 1830-1832 (2001) (Immunohistochemistry) 5. Beasley, Nigel J.P., et al. Cancer Research. 62: 2493-2497 (2002) (Immunohistochemistry) 6. Choke, E., Cockerill, G. W., Dawson, J., Chung, Y.-L., Griffiths, J., Wilson, R. W., Loftus, I. M., and Thompson, M. M. (2006) Hypoxia at the Site of Abdominal Aortic Aneurysm Rupture Is Not Associated with Increased Lactate. 1085: 306-310. 7. Khan, Z., Michalopoulos, G. K., and Stolz, D. B. (2006) Peroxisomal Localization of Hypoxia-Inducible Factors and Hypoxia-Inducible Factor Regulatory Hydroxylases in Primary Rat Hepatocytes Exposed to Hypoxia-Reoxygenation, 169, 1251-1269.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HIF1A / HIF1 alpha antibody

IHC: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - IHC staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining. Mouse IgG2b H1alpha67 Purified Bov, Ft, Hu, Mky, Ms, Por, Rb, Rt, Sh C, ICC/IF, IP, P, WB
0.1 ml / €410.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha antibody

Immunohistochemistry: HIF-1 alpha antibody [NB100-449] - Mouse Brain, Neurons 40X Rabbit Aff - Purified Hu, Ms C, F, P, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha antibody

Immunocytochemistry: HIF-1 alpha Antibody [NB100-479] - Detection of HIF-1 Alpha (green) in RCC4 cells using NB100-479.  Nuclei (Blue) were counterstained using Hoechst 33258. Rabbit Aff - Purified Ms C, P, WB
0.1 ml / €330.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha (329-530) antibody

Western blot analysis of HIF1 Alpha in HeLa cell lysates using a 1:500 dilution of the antibody Mouse IgG1 ESEE122 Purified Bov, Hu, Ms, Rt C, E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha (329-530) antibody

Western blot analysis of HIF1 Alpha in HeLa cell lysates using a 1:500 dilution of the antibody Mouse IgG1 ESEE122 Purified Bov, Hu, Ms, Rt C, E, ICC/IF, P, WB
25 µg / €190.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (809-826) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) using HIF1A antibody Rabbit IgG Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, L, Mky, Ms, Op, Platypus, Por, Prim, Rt, Tr, Xen P, WB
50 µg / €470.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha antibody

Immunofluorescence: HIF-1 alpha Antibody (ESEE122) - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using anti-HIF1A. 100X magnification. Blue dye is DAPI nuclear staining. Photos courtesy of Susan Alexander and Hattie Gresham, PhD. Mouse IgG1 ESEE122 Purified Bov, Can, Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

Western Blot: HIF-1 alpha Antibody (H1alpha67) - Upon quantification, 100ug of MEF cells (+/+,-/-) protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at 1:500. The secondary antibody was conjugated with HRP and was used at  1:2500. Mouse IgG2b H1alpha67 Purified Bov, Ft, Hu, Ms, Por, Prim, Rt, Sh IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

HIF1A antibody - middle region validated by WB using chicken liver Rabbit IgG Purified Bov, Can, Gt, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

Western Blot: HIF-1 alpha Antibody [TA336260] - Analysis of TA336260, anti-HIF-1 alpha, on normoxic and hypoxic nuclear rat cell lysates. Rabbit Purified Can, GP, Hu, Ms, Prim, Rt C, ChIP, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

Western Blot: HIF-1 alpha Antibody [TA336308] - Analysis of HIF-1 alpha in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen. Rabbit Purified Gt, Hst, Hu, Ms, Prim, Rb, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1A / HIF1 alpha antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and lung cancer tissues (right) using HIF1A antibody Cat.-No AM06608SU-N with DAB staining. Mouse IgG1 1A3 Ascites Hu, Mky, Ms E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha antibody

HIF1A antibody - middle region (ARP38054_P050) validated by WB using CoCl2 at 1:500.; HIF1A antibody - middle region (ARP38054_P050) in cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

HIF1A / HIF1 alpha antibody

Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) [NB100-123] - Staining of HIF-1 alpha in human kidney using NB100-123. Mouse IgG2b H1alpha67 Purified Bov, Ft, Hu, Mky, Ms, Por, Rb, Rt, Sh C, ICC/IF, P, WB
0.1 ml / €330.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha antibody

Western Blot: HIF-1 alpha Antibody (ESEE122) [NB100-131B] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy of Susan Alexander and Hattie Gresham, PhD. Mouse IgG1 ESEE122 Biotin Bov, Hu, Ms, Rt C, IP, P, WB
0.1 ml / €320.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha antibody

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) [HRP] [NB100-131H] Mouse IgG1 ESEE122 HRP Bov, Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha sampler antibody

Immunocytochemistry: HIF-1 alpha antibody [NB100-900WB] - Mouse Brain, Neurons 40X. NB100-449 Mouse IgG 4 clones WB
5 vials / €590.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha sampler antibody

Immunohistochemistry: HIF-1 alpha Antibody [NB100-901IHC] - Staining of placenta, villi using. Mouse IgG 2 clones Hu, Ms, Rt C, F, ICC/IF, IP, P, WB
4 vials / €510.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha (Sample Pack) antibody

Immunohistochimistry: HIF-1 alpha Antibody Pack 5 vials [NB100-905] - Staining of mouse brain, neurons using NB100-449. Hu, Ms C, ChIP, F, ICC/IF, IP, P, WB
5 vials / €590.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha antibody

Western Blot: HIF-1 alpha Antibody (HA111) - Westerm blot detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein. (explants were subjected to 21 and 2% oxygen for 4 hours). Mouse IgG2 HA111 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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