HIF1AN / FIH1 antibody

Principal name

HIF1AN / FIH1 antibody

Alternative names for HIF1AN / FIH1 antibody

Factor inhibiting HIF-1, Hypoxia-inducible factor 1-alpha inhibitor, Hypoxia-inducible factor asparagine hydroxylase

SwissProt ID

Q8BLR9 (Mouse), Q9NWT6 (Human)

Gene ID

55662 (HIF1AN)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Eq (Equine), Por (Porcine), Rb (Rabbit), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C)

Background of HIF1AN / FIH1 antibody

Hypoxia-inducible factor 1, alpha subunit inhibitor (HIF1AN), a member of the Fe2+ and 2-oxoglutarate-dependent dioxygenase superfamily, is protein that hydoxylates a specific asparagine residue (Asn-803) within the HIF1α C-terminal transactivation domain. In normoxia, the HIF1AN-mediated hydroxylation of the HIF1α transactivation domain results in blockage of the HIF1α-p300/CBP interaction and represses transcriptional activity of HIF1α-targeted genes. Recombinant human HIF1AN protein was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Fukuba H., et al. (2008) Neurosci Lett. 433(3):209-14.
Li J., et al. (2007) Mol Cell Biol. 27(20):7345-53.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HIF1AN / FIH1 antibody

Western blot analysis of extracts of various cell lines, using HIF1AN antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF1AN / FIH1 antibody

Immunohistochemistry: Factor Inhibiting HIF-1 Antibody (EPR3658) [NBP1-40688] -  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FIH1 Rabbit IgG EPR3658 Hu F, ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

HIF1AN / FIH1 antibody

Detection of FIH-1 in rat and human samples Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1AN / FIH1 antibody

Western Blot: Factor Inhibiting HIF-1 Antibody (FIH 162c) - Western blot analysis in A431 cell lysates. Mouse IgG1 162c Purified Hu C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF1AN / FIH1 antibody

Western blot analysis of FIH in a human cell line mix, using a 1:1000 dilution of the antibody Mouse IgG1 FIH162C Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF1AN / FIH1 antibody

WB Suggested Anti-HIF1AN Antibody Titration: 1.25ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF1AN / FIH1 antibody

WB Suggested Anti-HIF1AN Antibody Titration: 0.2-1 ug/ml; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

HIF1AN / FIH1 antibody

WB Suggested Anti-HIF1AN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Eq, Hu, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

HIF1AN / FIH1 antibody

WB Suggested Anti-HIF1AN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

HIF1AN / FIH1 (N-term) antibody

Western blot analysis of anti-HIF1AN (N-term) Pab in 293, HL60 and Jurkat cell line lysates. HIF1AN (N-term)(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HIF1AN / FIH1 (C-term) antibody

Western blot analysis of anti-HIF1AN (C-term) Pab in A375 and mouse brain cell line lysate. HIF1AN (C-term)(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HIF1AN / FIH1 antibody

HIF1AN / FIH1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

HIF1AN / FIH1 antibody

Western Blot analysis of HIF1AN expression in transfected 293T cell line (H00055662-T01) by HIF1AN MaxPab polyclonal antibody.

Lane 1: HIF1AN transfected lysate(38.39 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

HIF1AN / FIH1 antibody

Immunofluorescence of monoclonal antibody to HIF1AN on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 1D8 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HIF1AN / FIH1 antibody

Western blot analysis of HIF1AN polyclonal antibody ( Cat # PAB2198 ) in 293, HL-60 and Jurkat cell line lysates. HIF1AN ( N-term ) ( arrow ) was detected using the purified polyclonal antibody. Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

HIF1AN / FIH1 antibody

Western blot analysis of HIF1AN polyclonal antibody ( Cat # PAB2198 ) in A-375 and mouse brain cell line lysate. HIF1AN ( C-term ) ( arrow ) was detected using the purified polyclonal antibody. Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

HIF1AN / FIH1 antibody

Detection of HIF1AN in rat brain tissue using HIF1AN polyclonal antibody ( Cat # PAB12417 ) ( 1 : 100 ). Rabbit Hu, Ms, Rt C, IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

HIF1AN / FIH1 antibody

Human Jurkat; WB Suggested Anti-HIF1AN Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; HIF1AN antibody - N-terminal region (ARP32903_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

HIF1AN / FIH1 antibody

Human Heart; WB Suggested Anti-HIF1AN Antibody Titration: 0.2-1 ug/ml. Positive Control: Human heart; HIF1AN antibody - middle region (ARP32904_P050) in Human Heart cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

HIF1AN / FIH1 antibody

Human Jurkat; WB Suggested Anti-HIF1AN Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; HIF1AN antibody - N-terminal region (ARP39316_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

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