HIF2A / HIF2 alpha antibody

Principal name

HIF2A / HIF2 alpha antibody

Alternative names for HIF2A / HIF2 alpha antibody

BHLHE73, HIF-2 alpha, MOP2, PASD2, Hypoxia-inducible factor 2 alpha, HIF-1 alpha-like factor, HLF, HIF-related factor, EPAS1, Endothelial PAS domain-containing protein 1, Class E basic helix-loop-helix protein 73, Basic-helix-loop-helix-PAS protein MOP2

SwissProt ID

P97481 (Mouse), Q99814 (Human), Q9JHS1 (Rat)

Gene ID

13819 (Epas1), 2034 (EPAS1), 29452

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Fi (Fish), Bov (Bovine), Chk (Chicken), Por (Porcine), Xen (Xenopus), Ze (Zebrafish), Can (Canine), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP), ELISA (detection) (E(detection))

Background of HIF2A / HIF2 alpha antibody

HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88% identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event1. Moreover, the predicted protein shares 48% sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia2. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration4.

General readings

1. Fukuda R, Zhang H, Kim JW, Shimoda L, Dang CV, Semenza GL.
Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
1. Nakamura, K., et al. Brain-Derived Neurotrophic Factor Activation of TrkB Induces Vascular Endothelial Growth Factor Expression via Hypoxia-Inducible Factor-1alpha in Neuroblastoma Cells. American Association for Cancer Research 66, 4249-4255, 2006.
2. Kuznetsova, A.V., et al. von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination. PNAS. 100:2706-2711, 2003.
3. Schultz, K., Fanburg, B.L., Deasly, D. Hypoxia and hypoxia-inducible factor 1a promote growth factor-induced proliferation of human vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol. 2006.
4. Mascio, C.E., et al. Myocardial vascular and metabolic adaptations in chronically anemic fetal sheep. Am. J. Physiol. Regulatory Inegrative Comp. Physiol. 289:R1736-R1745, 2005.
5. Calvani, M., et al. Hypoxic induction of an HIF-1a-dependent bFGF autocrine loop drives angiogenesis in human endothelial cells. Blood. 107:2705-2712, 2006.
6. Mizukami, Y., et al. Hypoxic regulation of vascular endothelial growth factor through the induction of PI3K/Rho/ROCK and c-Myc. J. Biol. Chem. 2006.
7. Hui, A.S., et al. Calcium signaling stimulates translation of HIF-a during hypoxia. FASEB J. 20:466-475, 2006.
8. Shinojima, T., et al. Renal cancer cells lacking hypoxia inducible factor (HIF)-1alpha expression maintain vascular endothelial growth factor expression through HIF-2alpha.Carcinogenesis, March 2007;28: 529-536.
9. Inoue, Y., et al. Expression of hypoxia-inducible factor 1alpha and 2alpha in choroidal neovascular membranes associated with age-related macular degeneration. Vr. J. Ophthalmol, Dec 2007; 91:1720-1721.
10. Khacho, M., et al. Cancer-Causing Mutations in a Novel Transcription-Dependent Nuclear Export Motif of VHL Abrogate Oxygen-Dependent Degradation of Hypoxia-Inducible Factor. Mol. Cell. Biol. 28(1): 302-314, 2008.
11. Hill, P., et al. Inhibition of Hypoxia Inducible Factor Hydroxylases Protects Against Renal Ischemia-Reperfusion Injury. J. Am. Soc. Nephrol. Jan 2008; 19: 39 - 46.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HIF2A / HIF2 alpha antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPAS1 (RC208604, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPAS1. Mouse IgG1 OTI2G5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF2A / HIF2 alpha antibody

Western Blot: HIF-2 alpha antibody [NB100-132] - HIF-2 alpha detected in hypoxic human lysate using NB 100-132. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate. Mouse IgG ep190b Purified Hu, Rt F, P, WB
0.1 ml / €330.00
  Novus Biologicals Inc.

HIF2A / HIF2 alpha (202-240) antibody

Immunohistochemistry on Paraffin Sections: Rat Small Intestine Tissue stained with HIF-2A Polyclonal Antibody Rabbit IgG Aff - Purified Ms, Rt C, ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

HIF2A / HIF2 alpha antibody

Western Blot: HIF-2 alpha Antibody - Western blot analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using anti-EPAS1. Rabbit Purified Fi, Hu, Ms, Rt C, E, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF2A / HIF2 alpha (Sample Pack) antibody

Immunohistochemistry:  HIF-2 alpha Antibody Pack 3 vials [NB100-902HIF2] - Staining of heart, cardiac myocytes using NB100-132. Hu, Ms, Rt C, E, F, ICC/IF, P, WB
2 vials / €290.00
  Novus Biologicals Inc.

HIF2A / HIF2 alpha antibody

HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate Mouse IgG1 ep190b Purified Hu, Rt C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF2A / HIF2 alpha (535-631) antibody

Western blot analysis of HIF2 Alpha in CoCl treated HeLa lysates, using a 1:1000 dilution of the antibody Mouse IgG1 EP190B Purified Hu C, E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF2A / HIF2 alpha antibody

WB Suggested Anti-EPAS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

HIF2A / HIF2 alpha antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-EPAS1 mouse monoclonal antibody. (TA801749) Mouse IgG1 OTI2A6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF2A / HIF2 alpha (535-631) antibody

Western blot analysis of HIF2 Alpha in CoCl treated HeLa lysates, using a 1:1000 dilution of the antibody Mouse IgG1 EP190B Purified Hu C, E, ICC/IF, WB
25 µg / €190.00
  Acris Antibodies GmbH

HIF2A / HIF2 alpha (N-term) antibody

Western blot analysis of HIF2A / HIF2 alpha Antibody (N-term) Cat.-No AP51440PU-N in A549 cell line lysates (35ug/lane). This demonstrates the EPAS1 antibody detected the EPAS1 protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HIF2A / HIF2 alpha antibody

HIF2A / HIF2 alpha Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

HIF2A / HIF2 alpha antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between EPAS1 and SMAD3 HeLa cells were stained with anti-EPAS1 rabbit purified polyclonal 1:1200 and anti-SMAD3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

HIF2A / HIF2 alpha antibody

HIF2A / HIF2 alpha Mouse IgG1 1G2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HIF2A / HIF2 alpha antibody

HIF2A / HIF2 alpha Mouse IgG1 ep190b Hu, Ms, Rt F, P, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

HIF2A / HIF2 alpha antibody

Western blot analysis of EPAS1 in human cardiac myocytes stained with EPAS1 polyclonal antibody ( Cat # PAB12124 ). Rabbit Fi, Hu, Ms, Rt C, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

HIF2A / HIF2 alpha antibody

Immunohistochemical staining of Epas1 on formalin fixed, paraffin embedded eat small intestine with Epas1 polyclonal antibody ( Cat # PAB12333 ). Rabbit Ms, Rt C, ICC/IF, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

HIF2A / HIF2 alpha antibody

Human placenta; . Lanes :. Human placenta . Primary Antibody Dilution :. 1:200 . Secondary Antibody :. Anti-rabbit-HRP . Secondary Antibody Dilution :. 1:5000 . Gene Name :. Brown: EPAS1 Purple: Haemotoxylin . Submitted by :. EPAS1; EPAS1 antibody - middle region (ARP32253_P050) in Human placenta cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

HIF2A / HIF2 alpha antibody

HIF2A / HIF2 alpha Rabbit IgG Aff - Purified Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

HIF2A / HIF2 alpha antibody

HIF2A / HIF2 alpha Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

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