HIF2A / HIF2 alpha antibody

Principal name

HIF2A / HIF2 alpha antibody

Alternative names for HIF2A / HIF2 alpha antibody

BHLHE73, HIF-2 alpha, MOP2, PASD2, Hypoxia-inducible factor 2 alpha, HIF-1 alpha-like factor, HLF, HIF-related factor, EPAS1, Endothelial PAS domain-containing protein 1, Class E basic helix-loop-helix protein 73, Basic-helix-loop-helix-PAS protein MOP2

SwissProt ID

P97481 (Mouse), Q99814 (Human), Q9JHS1 (Rat)

Gene ID

2034 (EPAS1), 29452 (Epas1), 13819

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Fi (Fish), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of HIF2A / HIF2 alpha antibody

HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88% identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event1. Moreover, the predicted protein shares 48% sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia2. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration4.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Yi, X., et al. Science 329(5987):75-78(2010)
Bougatef, F., et al. PLoS ONE 5 (8), E12265 (2010)
Hossein Ghaderian, S.M., et al. Pathology 42(5):446-453(2010)
Mowat, F.M., et al. PLoS ONE 5 (6), E11103 (2010).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HIF2A / HIF2 alpha antibody

  EPAS1 mouse monoclonal antibody, clone OTI2G5 (formerly 2G5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPAS1 (RC208604, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPAS1. Mouse IgG1 OTI2G5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF2A / HIF2 alpha (202-240) antibody

Immunohistochemistry on Paraffin Sections: Rat Small Intestine Tissue stained with HIF-2A Polyclonal Antibody Rabbit IgG Aff - Purified Ms, Rt C, ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

HIF2A / HIF2 alpha antibody

  Rabbit Polyclonal HIF-2 alpha Antibody  
Western Blot: HIF-2 alpha Antibody - Western blot analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using anti-EPAS1. Rabbit Purified Fi, Hu, Ms, Rt C, E, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF2A / HIF2 alpha antibody

  Mouse Monoclonal Antibody against HIF-2 alpha (ep190b)  
HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate Mouse IgG1 ep190b Purified Hu, Rt C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIF2A / HIF2 alpha (535-631) antibody

  Mouse monoclonal HIF2 alpha Antibody  
Western blot analysis of HIF2 Alpha in CoCl treated HeLa lysates, using a 1:1000 dilution of the antibody Mouse IgG1 EP190B Purified Hu C, E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF2A / HIF2 alpha antibody

  Rabbit Polyclonal Anti-EPAS1 Antibody  
WB Suggested Anti-EPAS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

HIF2A / HIF2 alpha antibody

  Mouse monoclonal Anti-HIF2A Clone Hif2a 237  
HIF2A / HIF2 alpha Mouse IgG Hif2a 237 Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF2A / HIF2 alpha antibody

  Mouse monoclonal Anti-HIF2A Clone EP190b  
HIF2A / HIF2 alpha Mouse IgG1 ep190b Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF2A / HIF2 alpha antibody

  EPAS1 mouse monoclonal antibody, clone OTI2A6 (formerly 2A6)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-EPAS1 mouse monoclonal antibody. (TA801749) Mouse IgG1 OTI2A6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF2A / HIF2 alpha (N-term) antibody

Western blot analysis of HIF2A / HIF2 alpha Antibody (N-term) Cat.-No AP51440PU-N in A549 cell line lysates (35ug/lane). This demonstrates the EPAS1 antibody detected the EPAS1 protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

EPAS1 - human gene knockout kit via CRISPR

EPAS1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EPAS1 - human gene knockout kit via CRISPR

EPAS1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EPAS1 - human gene knockout kit via CRISPR

EPAS1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EPAS1 - human gene knockout kit via CRISPR

EPAS1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Epas1 - mouse gene knockout kit via CRISPR

Epas1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Epas1 - mouse gene knockout kit via CRISPR

Epas1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Epas1 - mouse gene knockout kit via CRISPR

Epas1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Epas1 - mouse gene knockout kit via CRISPR

Epas1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

EPAS1-knockout Cell lysate

EPAS1-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, EPAS1 (mGFP-tagged) - Human endothelial PAS domain protein 1 (EPAS1), 200 uL, >10^7 TU/mL

Lenti ORF particles, EPAS1 (mGFP-tagged) - Human endothelial PAS domain protein 1 (EPAS1), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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