HIF3A / HIF3 alpha antibody

Principal name

HIF3A / HIF3 alpha antibody

Alternative names for HIF3A / HIF3 alpha antibody

HIF-3 alpha, Hypoxia-inducible factor 3 alpha, BHLHE17, MOP7, PASD7, Member of PAS protein 7, IPAS, Basic-helix-loop-helix-PAS protein MOP7, Inhibitory PAS domain protein, Class E basic helix-loop-helix protein 17

SwissProt ID

Q0VBL6 (Mouse), Q9JHS2 (Rat), Q9Y2N7 (Human)

Gene ID

64344 (HIF3A), 53417 (Hif3a)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)

Background of HIF3A / HIF3 alpha antibody

One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits(HIF1-α, HIF2-α, or HIF3-α) bound to the aryl hydrocarbon receptor nuclear translocator(ARNT) that is also known as HIF1-β. HIF-α is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-α may be a marker for tumor growth and angiogenesis. Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive element, which contains the core recognition sequence 5'-TACGTG-3', in the cis-regulatory regions of hypoxia-inducible genes. Transcriptional activation by HIF is linked to its ability to recruit coactivator proteins such as CREB-binding protein (CBP), p300, steroid receptor coactivator-1, and translation initiation factor 2.

General readings

Kimura,K., (2006) Genome Res. 16 (1), 55-65

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HIF3A / HIF3 alpha (581-592) antibody

  Rabbit polyclonal HIF3 alpha antibody  
WB using Anti-Hif3A antibody shows detection of a band ~72 kDa corresponding to mouse Hif3A (arrowhead). Approximately 10 µg of a CoCl treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.  Treatment of exponentially growing 3T3 cells with 130 µM CoCl for 6 h at 37° C effectively mimics hypoxia. Primary antibody was diluted to 1:1,600. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX  was used at 1:10000. Rabbit Aff - Purified Ms, Rt E, ICC/IF, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

HIF3A / HIF3 alpha antibody

  Rabbit Polyclonal Anti-HIF3A Antibody  
WB Suggested Anti-HIF3A Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate; There is BioGPS gene expression data showing that HIF3A is expressed in HepG2 Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF3A / HIF3 alpha antibody

  HIF3A (HIF3 alpha) mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HIF3A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800720) Mouse IgG1 OTI2D2 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

HIF3A / HIF3 alpha antibody

  HIF3A mouse monoclonal antibody, clone OTI4C2 (formerly 4C2)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-HIF3A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800735) Mouse IgG1 OTI4C2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF3A / HIF3 alpha antibody

  HIF3A (HIF3 alpha) mouse monoclonal antibody, Clone , clone OTIF7 (formerly F7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIF3A (RC210255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIF3A. Mouse IgG1 OTIF7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF3A / HIF3 alpha antibody

  HIF3A (HIF3 alpha) mouse monoclonal antibody, Clone , clone OTIB7 (formerly B7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIF3A (RC210255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIF3A. Mouse IgG1 OTIB7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF3A / HIF3 alpha (323-376) antibody

Western blot analysis of HIF3A Antibody Cat.-No AP20606PU-N in extracts from Rat brain cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

HIF3A / HIF3 alpha (N-term) antibody

Paraffin Embedded Human Kidney stained with HIF3A / HIF3 alpha Antibody Cat.-No AP33233PU-N at 4 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Goat Aff - Purified Hu E, P
0.1 mg / €350.00
  Acris Antibodies GmbH

HIF3A / HIF3 alpha (N-term) antibody

Western blot analysis of HIF-3 alpha in Jurkat cell. Rabbit IgG Aff - Purified Ms WB
0.1 ml / €410.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

HIF3A - human gene knockout kit via CRISPR

HIF3A - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

HIF3A - human gene knockout kit via CRISPR

HIF3A - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

HIF3A - human gene knockout kit via CRISPR

HIF3A - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

HIF3A - human gene knockout kit via CRISPR

HIF3A - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hif3a - mouse gene knockout kit via CRISPR

Hif3a - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Hif3a - mouse gene knockout kit via CRISPR

Hif3a - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hif3a - mouse gene knockout kit via CRISPR

Hif3a - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hif3a - mouse gene knockout kit via CRISPR

Hif3a - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

HIF3A overexpression lysate

HIF3A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, HIF3A (mGFP-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, HIF3A (mGFP-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,510.00
  OriGene Technologies, Inc.

Lenti ORF particles, HIF3A (Myc-DDK-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, HIF3A (Myc-DDK-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,360.00
  OriGene Technologies, Inc.

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn