HIF3A / HIF3 alpha antibody

Principal name

HIF3A / HIF3 alpha antibody

Alternative names for HIF3A / HIF3 alpha antibody

HIF-3 alpha, Hypoxia-inducible factor 3 alpha, BHLHE17, MOP7, PASD7, Member of PAS protein 7, IPAS, Basic-helix-loop-helix-PAS protein MOP7, Inhibitory PAS domain protein, Class E basic helix-loop-helix protein 17

SwissProt ID

Q0VBL6 (Mouse), Q9JHS2 (Rat), Q9Y2N7 (Human)

Gene ID

64344 (HIF3A), 53417 (Hif3a)

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of HIF3A / HIF3 alpha antibody

One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits(HIF1-α, HIF2-α, or HIF3-α) bound to the aryl hydrocarbon receptor nuclear translocator(ARNT) that is also known as HIF1-β. HIF-α is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-α may be a marker for tumor growth and angiogenesis. Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive element, which contains the core recognition sequence 5'-TACGTG-3', in the cis-regulatory regions of hypoxia-inducible genes. Transcriptional activation by HIF is linked to its ability to recruit coactivator proteins such as CREB-binding protein (CBP), p300, steroid receptor coactivator-1, and translation initiation factor 2.

General readings

Kimura,K., (2006) Genome Res. 16 (1), 55-65

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HIF3A / HIF3 alpha (581-592) antibody

WB using Anti-Hif3A antibody shows detection of a band ~72 kDa corresponding to mouse Hif3A (arrowhead). Approximately 10 µg of a CoCl treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.  Treatment of exponentially growing 3T3 cells with 130 µM CoCl for 6 h at 37° C effectively mimics hypoxia. Primary antibody was diluted to 1:1,600. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX  was used at 1:10000. Rabbit Aff - Purified Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF3A / HIF3 alpha antibody

WB Suggested Anti-HIF3A Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate; There is BioGPS gene expression data showing that HIF3A is expressed in HepG2 Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIF3A / HIF3 alpha antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HIF3A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800720) Mouse IgG1 OTI2D2 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

HIF3A / HIF3 alpha antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-HIF3A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800735) Mouse IgG1 OTI4C2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF3A / HIF3 alpha antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIF3A (RC210255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIF3A. Mouse IgG1 OTIF7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF3A / HIF3 alpha antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIF3A (RC210255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIF3A. Mouse IgG1 OTIB7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HIF3A / HIF3 alpha (323-376) antibody

Western blot analysis of HIF3A Antibody Cat.-No AP20606PU-N in extracts from Rat brain cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

HIF3A / HIF3 alpha (N-term) antibody

Paraffin Embedded Human Kidney stained with HIF3A / HIF3 alpha Antibody Cat.-No AP33233PU-N at 4 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Goat Aff - Purified Hu E, P
0.1 mg / €340.00
  Acris Antibodies GmbH

HIF3A / HIF3 alpha antibody

Western Blot analysis of HIF3A expression in transfected 293T cell line (H00064344-T01) by HIF3A MaxPab polyclonal antibody.

Lane 1: HIF3A transfected lysate(40.04 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

HIF3A / HIF3 alpha antibody

Immunofluorescence of purified MaxPab antibody to HIF3A on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

HIF3A / HIF3 alpha antibody

Western blot using Hif3a polyclonal antibody ( Cat # PAB10271 ) shows detection of aband ~72 kDa corresponding to mouse Hif3a ( arrowhead ).
Approximately 10 µg of a CoCl2 treated NIH/3T3 cell lysate ( lane 1 ) and control NIH/3T3 cell lysate ( lane 2 ) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
Treatment of exponentially growing NIH/3T3 cells with 130 µM CoCl2 for 6 h at 37°C effectively mimicshypoxia.
After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,600.
The membrane was washed and reacted with a 1:10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Rabbit Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

HIF3A / HIF3 alpha antibody

Human kidney; HIF3A antibody - C-terminal region (ARP39936_T100) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified Hu P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

HIF3A / HIF3 alpha antibody

HIF3A / HIF3 alpha Rabbit IgG Aff - Purified Hu, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

HIF3A / HIF3 alpha (N-term) antibody

Western blot analysis of HIF-3 alpha in Jurkat cell. Rabbit IgG Aff - Purified Ms WB
0.1 ml / €410.00
  Neuromics Antibodies

HIF3A / HIF3 alpha antibody

Western Blot: HIF-3 alpha Antibody [NB100-2287] - Western blot analysis of HIF-3 alpha in Jurkat cell lysate using NB100-2287. Rabbit Aff - Purified Ms WB
0.1 ml / €360.00
  Novus Biologicals Inc.

HIF3A / HIF3 alpha antibody

HIF3A / HIF3 alpha Rabbit Biotin Ms WB
0.1 ml / €360.00
  Novus Biologicals Inc.

HIF3A / HIF3 alpha antibody

HIF3A / HIF3 alpha Rabbit DyLight488 Ms WB
0.1 ml / €390.00
  Novus Biologicals Inc.

HIF3A / HIF3 alpha antibody

HIF3A / HIF3 alpha Rabbit HRP Ms WB
0.1 ml / €380.00
  Novus Biologicals Inc.

HIF3A / HIF3 alpha antibody

HIF3A / HIF3 alpha Rabbit Aff - Purified Ms WB
25 µl / €160.00
  Novus Biologicals Inc.

HIF3A / HIF3 alpha antibody

Western Blot: HIF-3 alpha Antibody [NB100-2529] - Detection of HIF-3 alpha using NB100-2529. Lane 1: Cos7 (-) control Lane 2: Cos7 CoCl2 treated (+) control Rabbit Purified Hu, Ms ICC/IF, IP, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

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