HIP2 / UBE2K antibody

Principal name

HIP2 / UBE2K antibody

Alternative names for HIP2 / UBE2K antibody

HIP-2, Ubiquitin-conjugating enzyme E2 K, E2(25K), E2-25K, Ubiquitin-protein ligase, LIG, Ubiquitin carrier protein, Huntingtin-interacting protein 2

SwissProt ID

P61085 (Bovin), P61086 (Human), P61087 (Mouse)

Gene ID

3093 (UBE2K), 53323 (Ube2k)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Bt (Bat), Eq (Equine), Fi (Fish), Gt (Goat), Hst (Hamster), Mky (Monkey), Op (Opossum), Prim (Primate), Rb (Rabbit), Xen (Xenopus), Tr (Turkey), Bear

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of HIP2 / UBE2K antibody

The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene.
HIP2 belongs to the ubiquitin conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short lived and abnormal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid bearing macrophages through ubiquitination and subsequent degradation of p53.

General readings

1. Kalchman MA, Graham RK, Xia G, Koide HB, Hodgson JG, Graham KC, Goldberg YP, Gietz RD, Pickart CM, Hayden MR.
Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme. J Biol Chem. 1996 Aug 9;271(32):19385-94. PMID: 8702625

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HIP2 / UBE2K (C-term) antibody

  Goat Polyclonal Antibody against HIP2  
TA302847 staining (1.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HIP2 / UBE2K antibody

  Anti-UBE2K Rabbit Polyclonal Antibody  
Predicted band size: 22 kDa . Positive control: Human brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIP2 / UBE2K antibody

  Rabbit polyclonal UBE2K Antibody(N-term)  
Western blot analysis of lysates from Hela, 293 cell line, mouse brain tissue lysate, C2C12 cell line (from left to right), using UBE2K Antibody (N-term)(Cat.  #TA324679).  TA324679 was diluted at 1:1000 at each lane.  A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Rabbit IgG Purified Bov, Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HIP2 / UBE2K antibody

  Rabbit Polyclonal Anti-UBE2K Antibody - middle region  
WB Suggested Anti-UBE2K Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

HIP2 / UBE2K antibody

  Rabbit Polyclonal Anti-UBE2K Antibody - N-terminal region  
WB Suggested Anti-UBE2K Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

HIP2 / UBE2K (144-157) antibody

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Bt, Can, Chk, Eq, Fi, Gt, Hst, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

UBE2K - human gene knockout kit via CRISPR

UBE2K - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2K - human gene knockout kit via CRISPR

UBE2K - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

UBE2K - human gene knockout kit via CRISPR

UBE2K - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2k - mouse gene knockout kit via CRISPR

Ube2k - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ube2k - mouse gene knockout kit via CRISPR

Ube2k - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2k - mouse gene knockout kit via CRISPR

Ube2k - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ube2k - mouse gene knockout kit via CRISPR

Ube2k - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

HIP2 / UBE2K (transcript variant 1)

  Recombinant protein of human ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast) (UBE2K), transcript variant 1  
HIP2 / UBE2K Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

HIP2 / UBE2K (transcript variant 3)

  Recombinant protein of human ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast) (UBE2K), transcript variant 3  
HIP2 / UBE2K Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

HIP2 / UBE2K (1-200, His-tag)

HIP2 / UBE2K Human Purified > 95 % E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

HIP2 / UBE2K (1-200, His-tag)

HIP2 / UBE2K Human Purified > 95 % E. coli
50 µg / €370.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

UBE2K overexpression lysate

UBE2K overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

UBE2K-knockout Cell lysate

UBE2K-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, UBE2K (mGFP-tagged) - Human ubiquitin-conjugating enzyme E2K (UBE2K), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, UBE2K (mGFP-tagged) - Human ubiquitin-conjugating enzyme E2K (UBE2K), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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