HIRA / TUPLE1 antibody

Principal name

HIRA / TUPLE1 antibody

Alternative names for HIRA / TUPLE1 antibody

DGCR1, HIR, TUP1-like enhancer of split protein 1, HIR histone cell cycle regulation defective homolog A

SwissProt ID

P54198 (Human), P79987 (Chick), Q61666 (Mouse), Q8QFR2 (Xenla)

Gene ID

7290 (HIRA), 15260 (Hira)

Available reactivities

S. cerevisiae (Saccharomyces cerevisiae), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Xen (Xenopus), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)

Background of HIRA / TUPLE1 antibody

Hira (UniProtKB/Swiss-Prot entry P54198) is a histone chaperone that cooperates with ASF1A to promote replication-independent chromatin assembly. It is important for histone regulation and is required for the periodic repression of histone gene transcription during the cell cycle. Hira plays an important role in the formation of transcriptiolly silent senescence-associated heterochromatic foci (SAHF). SAHF, which contain heterochromatin proteins such as HP1, are believed to repress expression of proliferation-promoting genes, leading to the irreversible cell cycle changes that occur in senescent cells. Interaction between HIRA and ASF1A is the rate limiting step in the formation of SAHF. Hira is also thought to be involved in certain haploinsufficiency syndromes such as DiGeorge syndrome. Insufficient production of Hira protein may disrupt normal embryonic development.

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HIRA / TUPLE1 antibody

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using HIRA antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIRA / TUPLE1 antibody

HIRA Antibody (Center) (Cat. #TA324826) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HIRA antibody detected the HIRA protein (arrow). Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HIRA / TUPLE1 antibody

WB Suggested Anti-Hira Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Brain Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

HIRA / TUPLE1 antibody

WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Hira, diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right. Rabbit IgG Purified Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

HIRA / TUPLE1 antibody

Figure 1. Western blot analysis using TUP1 antibody (AM05331PU-N) on recombinant TUP1 protein (amino acids 1-200) expressed in Mav108 cells. Mouse IgG 10 Purified S. cerevisiae WB
0.1 mg / €360.00
  Acris Antibodies GmbH

HIRA / TUPLE1 antibody

Immunofluorescence of purified MaxPab antibody to HIRA on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

HIRA / TUPLE1 antibody

Western blot analysis of HIRA polyclonal antibody ( Cat # PAB4861 ) in HL-60 cell lysate ( 35 µg/lane ) . HIRA ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

HIRA / TUPLE1 antibody

Western blot was performed on whole cell lysates from mouse fibroblasts ( Lane 1, NIH/3T3 ) and embryonic stem cells ( Lane 2, E14Tg2a ) with Hira polyclonal antibody ( Cat # PAB14064 ), diluted 1 : 1,000 in BSA/PBS-Tween. Rabbit Ms E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

HIRA / TUPLE1 pThr555 antibody

Immunofluorescence analysis of HeLa cells, using HIRA (Phospho-Thr555) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF
50 µg / €205.00
  Assay Biotech

HIRA / TUPLE1 pThr555 antibody

Immunofluorescence analysis of HeLa cells, using HIRA (Phospho-Thr555) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

HIRA / TUPLE1 antibody

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using HIRA (Ab-555) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

HIRA / TUPLE1 antibody

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using HIRA (Ab-555) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

HIRA / TUPLE1 (Center) antibody

Mouse Brain; WB Suggested Anti-Hira Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Brain; Hira antibody - middle region (ARP37054_P050) in Mouse Brain cells using Western Blot Rabbit Aff - Purified Ms WB
50 µg / €350.00
  AVIVA Systems Biology

HIRA / TUPLE1 antibody

HIRA / TUPLE1 Rabbit IgG Aff - Purified Hu, Ms E, P
0.1 mg / €360.00
  Novus Biologicals Inc.

HIRA / TUPLE1 antibody

HIRA / TUPLE1 Rabbit IgG Aff - Purified Hu, Ms E, P
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

HIRA / TUPLE1

HIRA / TUPLE1 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

HIRA / TUPLE1

HIRA / TUPLE1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

HIRA / TUPLE1

HIRA / TUPLE1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

HIRA 293T Cell Transient Overexpression Lysate(Denatured)

HIRA 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

19 Item(s)

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