Histamine H3 receptor antibody

Principal name

Histamine H3 receptor antibody

Alternative names for Histamine H3 receptor antibody

HH3R, GPCR97, G-protein coupled receptor 97

SwissProt ID

P58406 (Mouse), Q9JI35 (Cavpo), Q9QYN8 (Rat), Q9Y5N1 (Human)

Gene ID

11255 (HRH3), 85268 (Hrh3)

Available reactivities

Hu (Human), Bt (Bat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Hst (Hamster), Mky (Monkey), Ms (Mouse), Op (Opossum), Rt (Rat), Tr (Turkey), Gorilla, Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)

Background of Histamine H3 receptor antibody

The histamine receptor H3 (HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. HRH3 has significant sequence homology to members of the biogenic amine subfamily of GPCRs. Most notable is an aspartic acid residue in the predicted third transmembrane domain, which is a hallmark of the biogenic amine receptor subfamily; this residue is the putative binding site for the primary amine. HRH3 shares 22% and 21.4% amino acid sequence homology with the H1 and H2 receptors, respectively. Expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Northern blot analysis of human tissues showed HRH3 expression only in the brain, with highest expression in the thalamus and caudate nucleus. Whereas Northern blot analysis did not detect HRH3 expression in any peripheral tissue examined, RT-PCR showed expression in human small intestine, testis, and prostate. In situ hybridization of rat brain sections showed that Hrh3 is abundantly expressed in brain. Hrh3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle. In addition, Hrh3 expression was found in the locus ceruleus and in the histaminergic cell bodies in the tuberomammillary nuclei.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histamine H3 receptor antibody

Anti-HRH3 / Histamine H3 Receptor antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

Histamine H3 receptor antibody

Sample (30 ug of whole cell lysate). A: A431 . B: H1299. 10% SDS PAGE. TA308494 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histamine H3 receptor antibody

Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. TA308704 diluted at 1:5000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histamine H3 receptor antibody

(0.1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histamine H3 receptor antibody

Anti-HRH3 / Histamine H3 Receptor antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bov, Bt, Can, Eq, Gorilla, GP, Hst, Hu, Mky, Ms, Op, Rt, Tr ICC/IF, P
50 µg / €345.00
  OriGene Technologies, Inc.

Histamine H3 receptor antibody

Anti-Histamine 3 Receptor / HRH3 antibody IHC of human brain, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 7-15 ug/ml. Rabbit IgG Purified Bov, Can, Eq, Gorilla, GP, Hst, Hu, Mky, Ms, Rt
50 µg / €345.00
  OriGene Technologies, Inc.

Histamine H3 receptor antibody

Western blot analysis of rat (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): 1, 2. Anti-H3 Histamine Receptor antibody, (1:200). 3, 4. Anti-H3 Histamine Receptor antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

Histamine H3 receptor antibody

Host: Rabbit; Target Name: HRH3; Sample Tissue: Hela Whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Histamine H3 receptor antibody

Host: Rabbit; Target Name: Hrh3; Sample Tissue: Rat Small Intestine lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Histamine H3 receptor antibody

Western blot (WB) analysis of Histamine H3 Receptor antibody (Cat.-No.: AP01216PU-N) in extracts from COLO205 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Histamine H3 receptor (C-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with HRH3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Histamine H3 receptor (C-term) antibody

HRH3 Polyclonal Antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Histamine H3 receptor antibody

Histamine H3 receptor Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Histamine H3 receptor antibody

Histamine H3 receptor Mouse IgG2a 1D7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Histamine H3 receptor antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with HRH3 polyclonal antibody ( Cat # PAB3659 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Histamine H3 receptor antibody

Western Blot analysis of HRH3 expression from rat brain tissue lyate with HRH3 polyclonal antibody ( Cat # PAB12272 ). Rabbit Hu, Ms, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Histamine H3 receptor antibody

Western blot analysis of extracts from COLO205 cells, using HRH3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Histamine H3 receptor antibody

Western blot analysis of extracts from COLO205 cells, using HRH3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

Histamine H3 receptor antibody

Histamine H3 receptor Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

Histamine H3 receptor antibody

Immunohistochemical analysis of Histamine H3 Receptor staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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