Histidyl-tRNA synthetase / HARS antibody

Principal name

Histidyl-tRNA synthetase / HARS antibody

Alternative names for Histidyl-tRNA synthetase / HARS antibody

HRS, HisRS, Histidine-tRNA ligase, JO1, Jo-1 antigen

SwissProt ID

P12081 (Human), Q2KI84 (Bovin), Q61035 (Mouse)

Gene ID

3035 (HARS)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)

Background of Histidyl-tRNA synthetase / HARS antibody

Histidyl-tRNA synthetase (HisRS, Jo-1) is an aminoacyl-tRNA synthetase that charges transfer RNAs (tRNAs) with a cognate histidine residue to produce histidyl-tRNA. Histidyl-tRNA is essential for the incorporation of histidine into proteins. Systemic autoimmune diseases lead to aminoacyl-tRNA synthetase autoantibodies that are directed against self structures. HisRS is an antigen in systemic autoimmune diseases such as rheumatic arthritis or polymyositis with associated interstitial lung disease, thrombocytopenia and Raynaud’s phenomenon (vasospastic attacks that cause blood vessels to constrict). HisRS autoantibodies are unlike anti-synthetase antibodies because they bind the nonlinear, exposed epitopes on native HisRS when the enzyme is complexed to tRNA(His).

General readings

O’Hanlon TP., et al. (2002) Biochem Biophys Res Commun. 294(3):609-14.

  1. Quick-Filters:
  2. Highly Validated (2)

Items 1 to 20 of 33 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histidyl-tRNA synthetase / HARS antibody

Immunofluorescence of purified MaxPab antibody to HARS on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

Histidyl-tRNA synthetase / HARS antibody

Immunofluorescence of purified MaxPab antibody to HARS on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Histidyl-tRNA synthetase / HARS antibody

Western blot analysis of extracts from HeLa cells, using HARS antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histidyl-tRNA synthetase / HARS antibody

WB Suggested Anti-HARS Antibody; Titration: 1.0 ug/ml; Positive Control: 293T Whole Cell HARS is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Histidyl-tRNA synthetase / HARS antibody

Immunohistochemistry (IHC) analyzes of HisRS antibody (Cat.-No.: AP06665PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Histidyl-tRNA synthetase / HARS (N-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HARS antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Histidyl-tRNA synthetase / HARS antibody

Histidyl-tRNA synthetase / HARS Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Histidyl-tRNA synthetase / HARS antibody

Immunoprecipitation of HARS transfected lysate using anti-HARS MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HARS MaxPab mouse polyclonal antibody (B01) (H00003035-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Histidyl-tRNA synthetase / HARS antibody

Immunofluorescence of monoclonal antibody to HARS on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 1C8 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Histidyl-tRNA synthetase / HARS antibody

HARS monoclonal antibody (M03), clone 4D4. Western Blot analysis of HARS expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 4D4 Purified Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Histidyl-tRNA synthetase / HARS antibody

Histidyl-tRNA synthetase / HARS Mouse IgG2a 4D4 Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Histidyl-tRNA synthetase / HARS antibody

Western blot analysis of HARS polyclonal antibody ( Cat # PAB2968 ) in K-562 cell line lysates ( 35 µg/lane ) .HARS ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Histidyl-tRNA synthetase / HARS antibody

Immunofluorescence analysis of HepG2/HeLa cells, using HARS Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

Histidyl-tRNA synthetase / HARS antibody

Immunofluorescence analysis of HepG2/HeLa cells, using HARS Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

Histidyl-tRNA synthetase / HARS (N-term) antibody

Human 293T; WB Suggested Anti-HARS Antibody. . Titration: 1.0 ug/ml. . Positive Control: 293T Whole Cell. HARS is supported by BioGPS gene expression data to be expressed in HEK293T.; HARS antibody - N-terminal region (ARP61337_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Histidyl-tRNA synthetase / HARS antibody

Immunohistochemical analysis of HARS staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Histidyl-tRNA synthetase / HARS antibody

Immunohistochemical analysis of HARS staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Histidyl-tRNA synthetase / HARS antibody

Immunohistochemical analysis of HARS staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Histidyl-tRNA synthetase / HARS antibody

Histidyl-tRNA synthetase / HARS Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Histidyl-tRNA synthetase / HARS antibody

Histidyl-tRNA synthetase / HARS Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Items 1 to 20 of 33 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn