Histone H2A.x antibody

Principal name

Histone H2A.x antibody

Alternative names for Histone H2A.x antibody

H2a/x, H2AFX, H2AX

SwissProt ID

O65759 (Cicar), P16104 (Human), P27661 (Mouse), Q6GM86 (Xenla)

Gene ID

3014 (H2AFX), 15270 (H2afx)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Bov (Bovine), Can (Canine), GP (Guinea Pig), Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish), African clawed frog, Prim (Primate)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), ChIP assay (ChIP), Frozen Sections (C), ELISA (detection) (E(detection))

Background of Histone H2A.x antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H2AFX is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.
Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histone H2A.x antibody

Western blot analysis of extracts from HT-29 cells, using Histone H2AX antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Western Blot of Rabbit anti-H2AXpS139 antibody. Lane 1: HeLa Lysate stimulated with adriamycin (24 hr). Lane 2: HeLa Lysate unstimulated. Load: 35 µg per lane. Primary antibody: H2AXpS139 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15.1 kDa, ~18 kDa for H2AXpS139. Other band(s): none. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Predicted band size: 15 kDa . Positive control: HepG2 and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane1,2: HepG2 and 293 cells Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Western blot analysis of lysates from 293, CEM, HepG2, Jurkat, Hela, Raji cell line(from left to right). using H2AFX Antibody (C-term)(Cat.  #TA325142). TA325142 was diluted at 1:2000 at each lane.  A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Mouse IgG1 938CT5.1.1 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

H2AFX Antibody (N-term) (Cat. #TA325173) western blot analysis in CEM,HepG2,Raji cell line lysates (35ug/lane).This demonstrates the H2AFX antibody detected the H2AFX protein (arrow). Mouse IgG1 980CT1.5.5 Purified Hu, Ms, Ze WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Western blot analysis of Histone H2A.X expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract, untreated (Lane 3) and UV treated (Lane 4) Jurkat cell extract were western blotted using anti-phospho-H2A.X (Ser 139) antibody, clone: 2F3. Mouse IgG1 2F3 Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Western Blot: H2AX Antibody [TA337174] - Analysis of MOLT4 lysate in the 1) absence, 2) presence of immunizing peptide using Histone H2A.x antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for th Rabbit Purified Hu, Prim P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Western Blot: H2AX Antibody [TA337175] - Analysis of MOLT4 lysate in the 1) absence and 2) presence of immunizing peptide using Histone H2A.x antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for Rabbit Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa. Mouse IgG2a 2A9-B5 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa. Mouse IgG1 3E11-1H6-G3 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa. Mouse IgG1 7G9-H4 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa. Mouse IgG1 8H4-1H11-F8 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

WB was performed on histone extracts (15 ug) from untreated U2OS cells (lane 1) or from U2OS cells treated with camptothecin (lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2A.XS139p. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H2A.XS139p. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:14,600. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.x antibody

Western blot analysis with extracts from human HT-29 cells using Histone H2A.X antibody (Cat.-No. AP01278PU-N). Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Histone H2A.x antibody

Immunohistochemistry (IHC) analyzes of Histone H2A.X antibody (Cat.-No.: AP01346PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Histone H2A.x pSer139 antibody

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X pSer139 antibody AP02515PU (Red). Rabbit Aff - Purified Hu ICC/IF, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Histone H2A.x pSer139 antibody

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X pSer139 antibody AP02515PU (Red). Rabbit Aff - Purified Hu ICC/IF, WB
50 µl / €240.00
  Acris Antibodies GmbH

Histone H2A.x antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Histone H2A.X antibody. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

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