Histone H2A.Z antibody

Principal name

Histone H2A.Z antibody

Alternative names for Histone H2A.Z antibody

H2AFZ, H2AZ, H2A/z

SwissProt ID

P0C0S4 (Bovin), P0C0S5 (Human), P0C0S6 (Mouse), P0C0S7 (Rat)

Gene ID

3015 (H2AFZ), 51788 (H2afz), 58940

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP), Immunoprecipitation (IP)

Background of Histone H2A.Z antibody

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the D into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of D to form one octameric nucleosome. Histone tails undergo numerous post-translatiol modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptiol activation or repression or other nuclear processes. In addition to the genetic code, combitions of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dymically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of the histone H2A variant H2A.Z is associated with the promoters of active genes.

General readings

Marques, M., et al. Epigenetics 5(4):267-272(2010)
Svotelis, A., et al. Cell Cycle 9(2):364-370(2010)
Thakar, A., et al. Biochemistry 48(46):10852-10857(2009)
Hardy, S., et al. PLoS Genet. 5 (10), E1000687 (2009)
Gevry, N., et al. Genes Dev. 23(13):1522-1533(2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histone H2A.Z antibody

Sample(30 ug of whole cell lysate). A:MOLT4. B:Raji. 12% SDS PAGE. TA308893 diluted at 1:3000 Rabbit IgG Aff - Purified Hu ChIP, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.Z antibody

Sample (30 ug of whole cell lysate). A: JurKat. 15% SDS PAGE. TA308894 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.Z antibody

ChIP assays using HeLa cells. Titration of 1, 2, 5 and 10ug antibody per ChIP was analysed. IgG (2 ug/IP) was used as negative control. qPCR was performed using primers specific for the promoters of ACTB and EIF4A2 genes, used as positive control targets and for the coding region of MYT1 gene, negative control target. Image shows the recovery (the relative amount of IP'd DNA compared to input DNA). These results confirm the observation that acetylation of H2A.Z is present at active promoters. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H2A.Z antibody

Histone H2A.Z Rabbit Purified Hu, Ms, Rt C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Histone H2A.Z (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with H2AFZ Antibody (C-term) Cat.-No AP51991PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Histone H2A.Z (acetyl K5) antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2A (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

Histone H2A.Z (acetyl K5) antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2A (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

Histone H2A.Z antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2A (Ab-5) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

Histone H2A.Z antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2A (Ab-5) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

Histone H2A.Z (C-term) antibody

Human HeLa; WB Suggested Anti-H2AFZ Antibody. . Titration: 1.0 ug/ml. . Positive Control: Hela Whole Cell; H2AFZ antibody - C-terminal region (ARP61353_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

Histone H2A.Z (N-term) antibody

Human 721_B; WB Suggested Anti-H2AFZ Antibody. . Titration: 1.0 ug/ml. . Positive Control: 721_B Whole Cell. H2AFZ is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; H2AFZ antibody - N-terminal region (ARP61354_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Histone H2A.Z AcK5 antibody

Western blot analysis of Histone H2A.Z (AcK5) expression in K562 TSA-treated (A); HeLa TSA-treated (B) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rt, Sh, Ze ICC/IF, P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

Histone H2A.Z AcK5 antibody

Western blot analysis of Histone H2A.Z (AcK5) expression in K562 TSA-treated (A); HeLa TSA-treated (B) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rt, Sh, Ze ICC/IF, P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

Histone H2A.Z AcK5 antibody

Western blot analysis of Histone H2A.Z (AcK5) expression in K562 TSA-treated (A); HeLa TSA-treated (B) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rt, Sh, Ze ICC/IF, P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Histone H2A.Z

Histone H2A.Z Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Histone H2A.Z (full length, N-term HIS tag)

Histone H2A.Z Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Histone H2A.Z (1-128, His-tag)

Histone H2A.Z Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

Histone H2A.Z (1-128, His-tag)

Histone H2A.Z Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

Histone H2A.Z

Histone H2A.Z Human
2 µg / €300.00
  Abnova Taiwan Corp.

Histone H2A.Z

Histone H2A.Z Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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