Histone H2A.Z antibody

Principal name

Histone H2A.Z antibody

Alternative names for Histone H2A.Z antibody

H2AFZ, H2AZ, H2A/z

SwissProt ID

P0C0S4 (Bovin), P0C0S5 (Human), P0C0S6 (Mouse), P0C0S7 (Rat)

Gene ID

3015 (H2AFZ), 51788 (H2afz), 58940

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP), Immunoprecipitation (IP)

Background of Histone H2A.Z antibody

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the D into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of D to form one octameric nucleosome. Histone tails undergo numerous post-translatiol modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptiol activation or repression or other nuclear processes. In addition to the genetic code, combitions of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dymically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of the histone H2A variant H2A.Z is associated with the promoters of active genes.

General readings

Marques, M., et al. Epigenetics 5(4):267-272(2010)
Svotelis, A., et al. Cell Cycle 9(2):364-370(2010)
Thakar, A., et al. Biochemistry 48(46):10852-10857(2009)
Hardy, S., et al. PLoS Genet. 5 (10), E1000687 (2009)
Gevry, N., et al. Genes Dev. 23(13):1522-1533(2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histone H2A.Z antibody

  Rabbit Polyclonal antibody to Histone H2A.Z (H2A histone family, member Z)  
Sample(30 ug of whole cell lysate). A:MOLT4. B:Raji. 12% SDS PAGE. TA308893 diluted at 1:3000 Rabbit IgG Aff - Purified Hu ChIP, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.Z antibody

  Rabbit Polyclonal antibody to Histone H2A.Z (H2A histone family, member Z)  
Sample (30 ug of whole cell lysate). A: JurKat. 15% SDS PAGE. TA308894 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H2A.Z antibody

  Rabbit Polyclonal H2A.Zac Antibody  
ChIP assays using HeLa cells. Titration of 1, 2, 5 and 10ug antibody per ChIP was analysed. IgG (2 ug/IP) was used as negative control. qPCR was performed using primers specific for the promoters of ACTB and EIF4A2 genes, used as positive control targets and for the coding region of MYT1 gene, negative control target. Image shows the recovery (the relative amount of IP'd DNA compared to input DNA). These results confirm the observation that acetylation of H2A.Z is present at active promoters. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H2A.Z antibody

Histone H2A.Z Rabbit Purified Hu, Ms, Rt C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Histone H2A.Z (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with H2AFZ Antibody (C-term) Cat.-No AP51991PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Histone H2A.Z (acetyl K5) antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2A (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €230.00
  Assay Biotech

Histone H2A.Z (acetyl K5) antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2A (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €330.00
  Assay Biotech

Histone H2A.Z antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2A (Ab-5) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €230.00
  Assay Biotech

Histone H2A.Z antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2A (Ab-5) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

H2afz - mouse gene knockout kit via CRISPR

H2afz - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Histone H2A.Z

  Recombinant protein of human H2A histone family, member Z (H2AFZ)  
Histone H2A.Z Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Histone H2A.Z (full length, N-term HIS tag)

  Recombinant protein of human H2A histone family, member Z (H2AFZ), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
Histone H2A.Z Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Histone H2A.Z (1-128, His-tag)

Histone H2A.Z Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

Histone H2A.Z (1-128, His-tag)

Histone H2A.Z Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

H2AFZ overexpression lysate

H2AFZ overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, H2AFZ (mGFP-tagged) - Human H2A histone family, member Z (H2AFZ), 200 uL, >10^7 TU/mL

Lenti ORF particles, H2AFZ (mGFP-tagged) - Human H2A histone family, member Z (H2AFZ), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, H2AFZ (Myc-DDK tagged) - Human H2A histone family, member Z (H2AFZ) , 200 uL, >10^7 TU/mL

Lenti ORF particles, H2AFZ (Myc-DDK tagged) - Human H2A histone family, member Z (H2AFZ) , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, H2afz (GFP-tagged) - Mouse H2A histone family, member Z (H2afz), 200 uL, >10^7 TU/mL

Lenti ORF particles, H2afz (GFP-tagged) - Mouse H2A histone family, member Z (H2afz), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, H2afz (Myc-DDK-tagged) - Mouse H2A histone family, member Z (H2afz), 200 uL, >10^7 TU/mL

Lenti ORF particles, H2afz (Myc-DDK-tagged) - Mouse H2A histone family, member Z (H2afz), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, H2afz (GFP-tagged ORF) - Rat H2A histone family, member Z (H2afz), 200 uL, >10^7 TU/mL

Lenti ORF particles, H2afz (GFP-tagged ORF) - Rat H2A histone family, member Z (H2afz), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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