Histone H2B type 1-D antibody

Principal name

Histone H2B type 1-D antibody

Alternative names for Histone H2B type 1-D antibody

H2B.b, H2B/b, H2B.1 B, HIRA-interacting protein 2, HIST1H2BD, H2BFB, HIRIP2

SwissProt ID

P58876 (Human)

Gene ID

3017 (HIST1H2BD)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB)

Background of Histone H2B type 1-D antibody

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the D into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of D to form one octameric nucleosome. Histone tails undergo numerous post-translatiol modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptiol activation or repression or other nuclear processes. In addition to the genetic code, combitions of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dymically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H2B is associated with active genes.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histone H2B type 1-D antibody

ChIP assays using HeLa cells: ChIP” kit on sheared chromatin from 10,000 cells using the SX-8G IP-Star automated system. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the inactive MYOD gene. Image shows the recovery, expressed as a % of input (the relative amount of IP'd DNA compared to input DNA after qPCR analysis). Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H2B type 1-D antibody

WB was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2 ) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK12ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H2B type 1-D antibody

WB was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2 ) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK15ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H2B type 1-D antibody

WB using the antibody against H2BS14p diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Mouse IgG1 Monoclol Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

4 Item(s)

per page
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