Histone H3.1 antibody

Principal name

Histone H3.1 antibody

Alternative names for Histone H3.1 antibody

H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, H3FA, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

SwissProt ID

P68431 (Human), P68432 (Bovin), P68433 (Mouse), Q6NWP9 (Human)

Gene ID

8350 (HIST1H3A), 104976714 (LOC104976714)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Dros (Drosophila), Hst (Hamster), Por (Porcine), Rb (Rabbit), S. cerevisiae (Saccharomyces cerevisiae), Chk (Chicken), Ze (Zebrafish), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ChIP assay (ChIP), Frozen Sections (C), Dot blot (Dot), Immunoprecipitation (IP)

Background of Histone H3.1 antibody

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of D wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-termil tails of core histones undergo various post-translatiol modifications, including acetylation, phosphorylation, methylation, and ubiquitition. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a domint role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kise haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during aphase.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histone H3.1 pSer10 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1 (phospho-Ser10) antibody AP02460PU. Rabbit Aff - Purified ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Histone H3.1 pSer10 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1 (phospho-Ser10) antibody AP02460PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

Histone H3.1 antibody

Immunoperoxidase of monoclonal antibody to HIST1H3D on formalin-fixed paraffin-embedded human lateral ventricle wall. [antibody concentration 3 ug/ml] Mouse IgG3 1D8 Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Histone H3.1 antibody

Western blot analysis of extracts from COLO205 cells, using Histone H3.1 (Ab-10) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Western blot analysis of extracts from A549 cells, using Histone H3 (Ab-3) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Western blot analysis of extracts from HuvEc cells treated with Serum (20%, 30mins), using Histone H3 (Ab-28) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Histone H3 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Western Blot of Rabbit anti-Histone 3 antibody. Lane 1: Raji lysate. Lane 2: HeLa lysate. Load: 15ug, 25ug respectively. Primary antibody: Histone 3 antibody at 1:2000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15.4 kDa, ~15.4 kDa for Histone 3. Other band(s): none. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Predicted band size: 15 kDa . Positive control: HepG2 and NIH/3T3 cell and Rat liver tissue lysate. Recommended dilution: 1/ 1000-2000. (Gel: 12%SDS-PAGE Lane1: HepG2 cell lysate Lane2: NIH/3T3 cell lysate Lane3: Rat liver tissue lysate Lysates: 30 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Predicted band size: 15 kDa . Positive control: 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 12%SDS-PAGE Lane1: 293 cells lysate Lane2: Treated with the peptide Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Western blot analysis of Acetyl-Histone H3 phosphorylation expression in TSA treated RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Histone H3.1 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Western blot analysis of Histone H3.1 expression in COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Asymmetric DiMethyl-Histone H3-R26 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Asymmetric DiMethyl-Histone H3-R17 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using MonoMethyl-Histone H3-R17 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Symmetric DiMethyl-Histone H3-R17 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R26 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

Western blot analysis of extracts of 293 cell line, using phospho-HIST1H3J-S10 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1 antibody

WB Suggested Anti-HIST1H3A Antibody; Titration: 1.0 ug/ml; Positive Control: Jurkat Whole Cell Rabbit IgG Purified Hu, Ms, Por, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

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