Histone H3.1t antibody

Principal name

Histone H3.1t antibody

Alternative names for Histone H3.1t antibody

HIST3H3, H3FT, H3t, H3/t, H3/g

SwissProt ID

Q16695 (Human)

Gene ID

8290 (HIST3H3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E)

Background of Histone H3.1t antibody

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of D wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-termil tails of core histones undergo various post-translatiol modifications, including acetylation, phosphorylation, methylation, and ubiquitition (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a domint role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kise haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during aphase (11).

General readings

1. Braunstein M, et al. Mol. Cell. Biol. 16:4349–56, 1996.
2. Workman JL, Kingston RE,. Annu. Rev. Biochem. 67:
545–579, 1998.
3. Cheung, P. et al. Cell103, 263–27, 2000.
4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005.
5. Albig W, et al. Hum Genet 97:486-491. 1996.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Histone H3 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using MonoMethyl-Histone H3-K4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using DiMethyl-Histone H3-K4 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli. using TriMethyl-Histone H3-K4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using MonoMethyl-Histone H3-K9antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using DiMethyl-Histone H3-K9 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using     MonoMethyl-Histone H3-K27 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using DiMethyl-Histone H3-K27 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using TriMethyl-Histone H3-K27 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using MonoMethyl-Histone H3-K36 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using DiMethyl-Histone H3-K36 antibody. Rabbit IgG Purified WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using TriMethyl-Histone H3-K36 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli. using MonoMethyl-Histone H3-K79 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using DiMethyl-Histone H3-K79 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using TriMethyl-Histone H3-K79 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Symmetric DiMethyl-Histone H3-R2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Symmetric DiMethyl-Histone H3-R8 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Asymmetric DiMethyl-Histone H3-R2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using MonoMethyl-Histone H3-R8 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H3.1t antibody

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using Asymmetric DiMethyl-Histone H3-R8 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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