Histone H3.2 antibody

Principal name

Histone H3.2 antibody

Alternative names for Histone H3.2 antibody

H3/m, H3/o, HIST2H3A

SwissProt ID

P84227 (Bovin), P84228 (Mouse), P84229 (Chick), Q71DI3 (Human), Q78E59 (Mouse)

Gene ID

126961 (HIST2H3C), 333932 (HIST2H3A)

Available reactivities

Ce (C. elegans), Chk (Chicken), Dros (Drosophila), Hu (Human), Mam (Mammalia), Ms (Mouse), Pl (Plant), Rt (Rat), Xen (Xenopus), Bov (Bovine)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of Histone H3.2 antibody

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimethylation of H3 Arg8 (H3 R8Me2) is known as a mark of transcriptional repression. The protein arginine methyltransferases PRMT5 and PRMT2 can both methylate Arg8, with PRMT2 specifically methylating in an asymmetric manner (PMID 20708585). In addition, asymmetric dimethylation of Arg8 inhibits H3K9 methylation by G9a, but not symmetric Arg8Me2 does not (PMID 18438403).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histone H3.2 antibody

Fluorescent Immunohistochemistry : NIH/3T3 cells were cultured in chamber, and fixed onto slide followed by incubation with 5 µg/ml HIST2H3C polyclonal antibody ( Cat # PAB12679 ), then FITC labeled Goat anti Rabbit IgG for visualization under fluorescent microscope. Rabbit IgG Bov, Chk, Hu, Ms, Rt C, ChIP, E, ICC/IF, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Histone H3.2 antibody

The nuclear extract derived from HeLa were immunoprecipitated by 4 µg of HIST2H3C polyclonal antibody ( Cat # PAB12691 ), then probed with HIST2H3C polyclonal antibody at 1 : 1000. An immunoreactive band at ~17kDa is observed.
Note : ~50 kDa and ~22 kDa are the rabbit immunoglobulin heavy and light chains. Rabbit IgG Bov, Chk, Hu, Ms, Rt C, E, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Histone H3.2 (N-term HIS tag)

Histone H3.2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Histone H3.2 (full length, N-term HIS tag)

Histone H3.2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Histone H3.2

Histone H3.2 Human
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

HIST2H3A overexpression lysate

HIST2H3A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

HIST2H3C overexpression lysate

HIST2H3C overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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