Histone H3.3 antibody

Principal name

Histone H3.3 antibody

Alternative names for Histone H3.3 antibody

H3F3A, H3.3A, H3F3, PP781

SwissProt ID

P84243 (Human), P84244 (Mouse), P84245 (Rat), P84246 (Rabit), P84247 (Chick)

Gene ID

3020 (H3F3A), 8290 (HIST3H3), 3021 (H3F3B), 10888 (GPR83), 100361558 (LOC100361558)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Bov (Bovine), Chk (Chicken), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ChIP assay (ChIP), Dot blot (Dot), Frozen Sections (C)

Background of Histone H3.3 antibody

Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.

General readings

Tagami H., et al. (2004) Cell. 116:51-61Daury L N., et al. (2006) EMBO Rep. 7:66-71

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histone H3.3 antibody

Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. TA308895 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.3 antibody

Immunofluorescence with the Histone H3 ( K4me3 ) polyclonal antibody ( Cat # PAB0650 ).
HeLa cells were stained with antibody directed against modified histone and with DAPI.
Cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS.
The fixation step stabilises the morphology of the cells and permeabilizes membranes as well.
( A ) Cells were immunofluorescently labelled with the Histone H3 ( K4me3 ) polyclonal antibody ( Cat # PAB0650 ) ( diluted 1 : 200 and incubated for 1 hour at room temperature ) followed by goat anti-rabbit antibody conjugated to FITC.
( B ) Nuclei were DAPI stained to label specifically the DNA.
In both cases, the nuclei are clearly stained. Rabbit IgG Purified Hu ChIP, Dot, E, ICC/IF
50 µg / €560.00
  Abnova Taiwan Corp.

Histone H3.3 antibody

Western blot analysis using the Histone H3 ( K9me2 ) polyclonal antibody ( Cat # PAB0653 ).
Western blot was performed using histone extracts from HeLa cells ( HeLa NE, 15 µg ) and the Histone H3 ( K9me2 ) polyclonal antibody ( Cat # PAB0653 ) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.
The location of the protein of interest is indicated with an arrow. Rabbit IgG Purified Hu ChIP, Dot, E, WB
50 µg / €470.00
  Abnova Taiwan Corp.

Histone H3.3 antibody

NB4 cells were treated with ATRA for 168h ( Lane 1 ) and 24h ( Lane 2 ) to induce cell differentiation and NB4 cells were non-treated ( Lane 3 ). Histone ( acid ) extracts of treated and non-treated cells were analysed by Western blot using the Histone H3 (K9me3) polyclonal antibody ( Cat # PAB0654 ) at a dilution of 1 : 1,000. Rabbit IgG Purified Hu ChIP, Dot, E, WB
50 µg / €470.00
  Abnova Taiwan Corp.

Histone H3.3 antibody

Western blot analysis using the Histone H3 ( K36me3 ) polyclonal antibody ( Cat # PAB0656 ).
Mouse embryonic stem cells ( ES14 ) cells were treated or not with all-trans retinoic acid ( ATRA ).
The ATRA treatment is used to induce cell differentiation.
Histone ( acid ) extracts of treated ( lane 1 ) and non-treated cells ( lane 2 ).
Using Histone H3 ( K36me3 ) polyclonal antibody ( Cat # PAB0656 ) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk. The location of the protein of interest is indicated with an arrow. Rabbit IgG Purified Hu ChIP, Dot, E, WB
50 µg / €470.00
  Abnova Taiwan Corp.

Histone H3.3 antibody

Detection of Histone H3 in lysates, Lane 1 MEF, Lane 2 HeLa Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.3 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Histone H3.3 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H3.3 antibody

Adult rat hippocampus immunohistochemistry (with antigen retrieval via pepsin pretreatment) Mouse IgG1 N158/28 Supernatant Hu, Ms, Rt C, E
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

Histone H3.3 antibody

Adult rat hippocampus immunohistochemistry (with antigen retrieval via pepsin pretreatment) Mouse IgG1 N158/28 Purified Hu, Ms, Rt C, E
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

Histone H3.3 antibody

Immunohistochemistry (IHC) analyzes of Histone H3.3 antibody (Cat.-No.: AP06614PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P
0.1 mg / €350.00
  Acris Antibodies GmbH

Histone H3.3 antibody

Histone H3.3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Histone H3.3 antibody

Western analysis of extracts from HL-60 cells treated with 100 nM of calyculin using H3F3A monoclonal antibody, clone 44AT53.87 ( Cat # MAB1148 ) . Mouse IgG1 44AT53.87 Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Histone H3.3 antibody

Western analysis of extracts from HL-60 cells treated with 100 nM of calyculin using H3F3A monoclonal antibody, clone 44AT1232.94 ( Cat #MAB1149 ) . Mouse IgG1 44AT1232.94 Hu, Ms E, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

Histone H3.3 antibody

ChIP results obtained with the Histone H3 (K9ac) polyclonal antibody ( Cat # PAB0651 ) . ChIP assays were performed using U-2 OS cells. Rabbit IgG Purified Hu ChIP, Dot, E, ICC/IF
50 µg / €470.00
  Abnova Taiwan Corp.

Histone H3.3 antibody

Immunofluorescence with the Histone H3 ( K9/14ac ) polyclonal antibody ( Cat # PAB0652 ).
HeLa cells were stained with antibody directed against modified histone and with DAPI.
Cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS.
The fixation step stabilises the morphology of the cells and permeabilizes membranes as well.
( A ) Cells were immunofluorescently labelled with the Histone H3 ( K9/14ac ) polyclonal antibody ( Cat # PAB0652 ) ( diluted 1 : 200 and incubated for 1 hour at room temperature ) followed by goat anti-rabbit antibody conjugated to FITC.
( B ) Nuclei were DAPI stained to label specifically the DNA.
In both cases, the nuclei are clearly stained. Rabbit IgG Purified Hu ChIP, Dot, E, ICC/IF
50 µg / €470.00
  Abnova Taiwan Corp.

Histone H3.3 antibody

Histone H3.3 Rabbit IgG Purified Hu ChIP, Dot, E, WB
50 µl / €470.00
  Abnova Taiwan Corp.

Histone H3.3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the H3F3A/H3F3B polyclonal antibody ( Cat # PAB2226 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit IgG Purified Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

Histone H3.3 antibody

Western blot analysis of H3F3A/H3F3B polyclonal antibody ( Cat # PAB2227 ) in CEM cell line lysates ( 35 µg/lane ) . Histone H3 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 30 dilution ) . Rabbit IgG Purified Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Histone H3.3 pSer31 antibody

Immunofluorescence analysis of HeLa cells, using Histone H3.3 (Phospho-Ser31) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €205.00
  Assay Biotech

Histone H3.3 pSer31 antibody

Immunofluorescence analysis of HeLa cells, using Histone H3.3 (Phospho-Ser31) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

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