Histone H3 antibody

Principal name

Histone H3 antibody

Alternative names for Histone H3 antibody

H3 Histone family, HIST1H3

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Dros (Drosophila), All Species, Chk (Chicken), Pl (Plant), Xen (Xenopus), Mam (Mammalia), Ye (Yeast), Hst (Hamster), Ce (C. elegans), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Sheep, Rat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ChIP assay (ChIP), Enzyme Immunoassay (E), Flow Cytometry (F), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Dot blot (Dot)

Background of Histone H3 antibody

In eukaryotic cells, DNA is associated with histones and other proteins to form chromatin. The cell division cycle constitutes a series of processes that have evolved to create two genetically identical daughter cells from a mother cell. One of these processes is the conversion of relatively amorphous, extended interphase chromatin into condensed, highly ordered mitotic chromosomes. Proper mitotic chromosome condensation is essential for the correct segregation of sister chromatids into two daughter cells. The basic unit of chromatin is the nucleosome core particle, which consists of 140 bp DNA wrapped around an octameric core containing two each of the four conserved core histones: H2A, H2B, H3 and H4. A fifth histone, the linker histone H1, interacts with DNA of variable length, linking adjacent nucleosome cores, and further compacting the chromatin. Chromatin changes are initiated during G2 phase of the cell cycle, in preparation for cell division. The most striking morphological change is chromatin condensation, which becomes apparent during prophase and is maximal during the subsequent stages of mitosis. Histone H1 and the N-terminal tail of H3 have key roles in the folding and inter-association of the chromatin fiber. Two currently known phosphorylation sites are present in the N-terminus of H3; serine-10 and serine-28.1,2 Mitogenic stimulation, oncogenic transformation, or induction of oncogenic ras expression are accompanied with increase in serine-10 phosphorylation of the H3 N-terminal domain. Indeed, it has been shown that phosphorylated H3 is associated with c-fos and c-myc genes in stimulated cells. Phosphorylation of H3, at both serine-10 and -28, coincides with the induction of mitotic chromosome condensation. H3 phosphorylation may contribute to proto-oncogene induction by modulating chromatin structure and releasing blocks in elongation. In contrast to H1 hyperphosphorylation, site-specific phosphorylation of core histone H3 at serine-10 and -28 appears to occur exclusively during mitosis in mammalian cells. H3 dephosphorylation occurs quite rapidly after mitosis and serine-10/28 remain unphosphorylated throughout the remainder of interphase. PP1 has been identified as the H3 phosphatase. Monoclonal antibodies reacting specifically with phosphorylated histone H3, are useful tools to study molecular mechanisms associated with the G2 to M transition and chromatin condensation, and for the analysis of protein kinase(s) and phosphatase(s) involved in H3 phosphorylation or dephosphorylation. They may also be used in multiparameter analysis to relate H3 phosphorylation in individual cells to the cell

General readings

General / background references:Strahl BD & Allis CD The language of covalent histone modifications. Nature 403:41-5 (2000). PubMed PMID: 10638745
Cheung P et al. Signaling to chromatin through histone modifications. Cell 103:263-71 (2000). PubMed PMID: 11057899
Kornberg RD & Lorch Y Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome. Cell 98:285-94 (1999). PubMed PMID: 10458604
Thomson S et al. MAP kinase-mediated signalling to nucleosomes and immediate-early gene induction. Semin Cell Dev Biol 10:205-14 (1999). PubMed PMID: 10441074
Chadee DN et al. Increased Ser-10 phosphorylation of histone H3 in mitogen-stimulated and oncogene-transformed mouse fibroblasts. J Biol Chem 274:24914-20 (1999). PubMed PMID: 10455166
Sassone-Corsi P et al. Requirement of Rsk-2 for epidermal growth factor-activated phosphorylation of histone H3. Science 285:886-91 (1999). PubMed PMID: 10436156
Thomson S et al. The nucleosomal response associated with immediate-early gene induction is mediated via alternative MAP kinase cascades: MSK1 as a potential histone H3/HMG-14 kinase. EMBO J 18:4779-93 (1999). PubMed PMID: 10469656
De Cesare D et al. Rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of CREB protein and transcription of c-fos gene. Proc Natl Acad Sci U S A 95:12202-7 (1998). PubMed PMID: 9770464
Struhl K Histone acetylation and transcriptional regulatory mechanisms. Genes Dev 12:599-606 (1998). PubMed PMID: 9499396
Zhang W et al. Essential and redundant functions of histone acetylation revealed by mutation of target lysines and loss of the Gcn5p acetyltransferase. EMBO J 17:3155-67 (1998). PubMed PMID: 9606197
Kuo MH et al. Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines. Nature 383:269-72 (1996). PubMed PMID: 8805705
Sobel RE et al. Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4. Proc Natl Acad Sci U S A 92:1237-41 (1995). PubMed PMID: 7862667
Barratt MJ et al. Mitogen-stimulated phosphorylation of histone H3 is targeted to a small hyperacetylation-sensitive fraction. Proc Natl Acad Sci U S A 91:4781-5 (1994). PubMed PMID: 8197135
Mahadevan LC et al. Rapid histone H3 phosphorylation in response to growth factors, phorbol esters, okadaic acid, and protein synthesis inhibitors. Cell 65:775-83 (1991). PubMed PMID: 2040014

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histone H3 (Monomethyl K4) antibody

Immunohistochemistry-Paraffin: Histone H3 [Me-Lys4] Antibody [NB100-2209] - Anti-Methyl-Histone H3 [Me-Lys4]: Immunoperoxidase staining of Anti-Methyl-Histone H3 [Me-Lys4] in formalin fixed, paraffin-embedded mouse testis at 0.1 µg/mL. Rabbit Aff - Purified Bov, Dros, Hu, Ms, Rt ChIP, P, WB
50 µl / €340.00
  Novus Biologicals Inc.

Histone H3 antibody

Immunohistochemistry: Histone H3 Antibody [NB500-171]-Immunoperoxidase staining of Anti-Histone H3 in formalin fixed, paraffin-embedded mouse testis at 0.2 ug/mL. Rabbit Purified Chk, Dros, Hu, Ms, Pl, Rt, Xen P, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Histone H3 (Trimethyl K9) antibody

Immunocytochemistry: Histone H3 [trimethyl Lys9] Antibody (6F12-H4) [NBP1-30436] – Histone H3 [trimethyl lys9] staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue) Mouse IgG1 6F12-H4 Purified Ce, Dros, Hu, Mam, Ms, Rt, Ye ChIP, E, ICC/IF, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Histone H3 (Citrulline Arg2, 8, 17) antibody

Immunodetection of Human Histone H3 / Citruline 2-8-17 using AM10179PU-N at 1/100000 dilution Mouse IgG1 7C10 Purified Hu E, F, ICC/IF, WB
0.1 mg / €410.00
  Acris Antibodies GmbH

Histone H3 pSer10 antibody

Figure 1. Western analysis of extracts from HL60 cells treated with 100nM of calyculin using Histone H3-pS10 Antibody (cat. #AM11033PU-N) Mouse IgG1 44AT1032.72 Purified Hu, Ms WB
0.1 ml / €370.00
  Acris Antibodies GmbH

Histone H3 pSer10 antibody

Figure 1. Western analysis of extracts from HL60 cells treated with 100nM of calyculin using Histone H3-pS10 Antibody (Cat. #AM11034PU-N). Mouse IgG1 44AT53.87 Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Histone H3 pSer10 antibody

Figure 1. Western analysis of extracts from HL60 cells treated with 100nM of calyculin using Histone H3-pS10 Antibody (Cat. #AM11035PU-N). Mouse IgG1 44AT1232.94 Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Histone H3 antibody

Immunohistochemistry (IHC) analyzes of Histone H3 antibody (Cat.-No.: AP06394PU-N ) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Histone H3 pSer10 / pSer28 antibody

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with PHH3 antibody Cat.-No. AP10439PU-N using peroxidase conjugate and DAB chromogen. Note nuclear staining of mitotic lymphocytes. Rabbit Purified Hu P
1 ml / €350.00
  Acris Antibodies GmbH

Histone H3 (Dimethyl K9) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Histone H3 (N-term) antibody

Western blot analysis of Histone H3 (N-term) (Cat.#AP11070PU-N) in CEM cell line lysates (35ug/lane). Histone H3(arrow) was detected using the purified Pab (1:30 dilution). Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Histone H3 pSer10 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Histone H3 pSer10 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Histone H3 pSer28 antibody

Western blot analysis of anti-Phospho-H3-pSer28 Pab (Cat. #AP12548PU-N) in HepG2 cell line lysate (35ug/lane). Mouse Phospho-H3-pS28(arrow) was detected using the purified Pab (1:64 dilution). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Histone H3 antibody

Western blot analysis of Histone H3 antibody (AP20240PU-N) in extracts from RAW264.7cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Histone H3 antibody

Immunohistochemistry analyzes of Histone H3 antibody (AP20241PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Histone H3 antibody

Immunohistochemistry analyzes of Histone H3 antibody (AP20242PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Histone H3 antibody

Immunohistochemistry analyzes of Histone H3 antibody (Cat.-No.: AP20304PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Histone H3 antibody

Immunohistochemistry (IHC) analyzes of Histone H3 antibody (Cat.-No.: AP21088PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Histone H3 antibody

Immunohistochemical analysis using Histone H3 antibody Cat.-No AP31733PU-N at 5 µg/ml on Human Prostate tissue (Formalin-fixed, Paraffin-embedded). Sheep IgG Purified Bov, Hu ICC/IF, IP, P, WB
0.125 mg / €470.00
  Acris Antibodies GmbH

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