Histone H4 antibody

Principal name

Histone H4 antibody

Alternative names for Histone H4 antibody

HIST1H4, H4/A, H4FA

SwissProt ID

P62801 (Chick), P62802 (Pig), P62803 (Bovin), P62804 (Rat), P62805 (Human), P62806 (Mouse)

Gene ID

121504 (HIST4H4), 100360950 (LOC100360950), 8359 (HIST1H4A)

Available reactivities

Hu (Human), Amph (Amphibian), Dros (Drosophila), Mam (Mammalia), Ye (Yeast), Pl (Plant), Ms (Mouse), Rt (Rat), Ce (C. elegans), Bov (Bovine), Chk (Chicken), Xen (Xenopus), Hst (Hamster), Por (Porcine), Mky (Monkey), Can (Canine), GP (Guinea Pig), Gt (Goat), Ze (Zebrafish)

Available hosts

Rat, Rabbit, Mouse, Sheep

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP), Paraffin Sections (P), Dot blot (Dot), Frozen Sections (C), Flow Cytometry (F)

Background of Histone H4 antibody

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, phosphorylation of H4 Ser1 (H4 pS1) has been linked to mitosis and DNA repair. This modification is enriched in sites proximal to double stranded brakes, but not those associated with UV damage. Casein kinase II (CK2) phosphorylates H4 Ser1, and it also implicated in regulating the DNA damage response. Furthermore, recruitment of CK2 requires the SIN3/RPD3 histone deacetylase complex.

General readings

1. Turner, B.M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabelling with site-specific antibodies.
FEBS Letters 253: 141-145.
2. Turner, B.M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei. Cell 69:
375-384.
3. Belyaev, N.D. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells. Hum. Genet. 97: 573-578.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histone H4 antibody

Western blot analysis using the Histone H4 ( K20me3 ) polyclonal antibody ( Cat # PAB0657 ).
Histone ( acid ) extracts of NB4 cells were analysed by Western blot using the Histone H4 ( K20me3 ) polyclonal antibody ( Cat # PAB0657 ) at a dilution of 1 : 750 in TBS-Tween + 5% skimmed milk. The location of the protein of interest is indicated. Rabbit IgG Purified Hu ChIP, Dot, E, WB
50 µl / €470.00
  Abnova Taiwan Corp.

Histone H4 antibody

Immunocytochemistry: Histone H4 [p Ser1] Antibody [NB21-2000] - Histone H4 pS1 antibody was tested in in nonmitotic, prophase, and telophase HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red). Rabbit Aff - Purified Hu, Mam, Ms, Rt ChIP, Dot, ICC/IF, WB
50 µg / €410.00
  Novus Biologicals Inc.

Histone H4 antibody

Immunocytochemistry: Histone H4 [ac  Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 594 (red). Rabbit Aff - Purified Hu, Mam, Ms, Rt ChIP, Dot, ICC/IF, WB
50 µg / €410.00
  Novus Biologicals Inc.

Histone H4 antibody

Immunocytochemistry: Histone H4 [Monomethyl Lys20] Antibody [NB21-2088] - Histone H4 K20me1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue). Rabbit Aff - Purified Ce, Chk, Dros, Hu, Mam, Ms, Pl, Rt, Xen ChIP, Dot, ICC/IF, WB
50 µg / €400.00
  Novus Biologicals Inc.

Histone H4 antibody

Histone H4 Rabbit Aff - Purified Ce, Chk, Dros, Hu, Mam, Ms, Pl, Rt, Xen ChIP, Dot, ICC/IF, WB
50 µg / €400.00
  Novus Biologicals Inc.

Histone H4 antibody

Immunocytochemistry: Histone H4 [monomethyl Lys20] Antibody (5E10-D8) [NBP1-30436] – Histone H4 [methyl Lys20] staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue). Mouse IgG1 5E10-D8 Purified Ce, Dros, Hu, Mam, Ms, Rt, Ye ChIP, Dot, E, ICC/IF, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

Histone H4 antibody

Immunohistochemistry-Paraffin: histone h4 Antibody (EPR1004) [NBP1-96054] - Histone H4 Acetyl (K16). Rabbit EPR1004 Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Histone H4 antibody

Western blot analysis of Histone H4 expression with rat kidney tissue lysate. Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of Acetyl-Histone H4 phosphorylation expression in TSA treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of Histone H4 expression in TSA treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using MonoMethyl-Histone H4-K20 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using TriMethyl-Histone H4-K20 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using Asymmetric DiMethyl-Histone H4-R3 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using Symmetric DiMethyl-Histone H4-R3 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4K20me2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Host: Rabbit; Target Name: HIST1H4A; Sample Tissue: 293T Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, GP, Gt, Ms, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB using the antibody against H4K20me1 diluted 1:750 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB using the antibody against H4K20me3, diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB using the antibody against H4K8ac diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right. Lane 2 shows the result of the Western analysis with the antibody; lane 1 shows the same analysis after incubation of the antibody with 750 pmol blocking peptide for 1 hour at room temperature. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2 ) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5,8,12ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu, Ms E
50 µg / €325.00
  OriGene Technologies, Inc.

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