Histone H4 antibody

Principal name

Histone H4 antibody

Alternative names for Histone H4 antibody

HIST1H4, H4/A, H4FA

SwissProt ID

P62801 (Chick), P62802 (Pig), P62803 (Bovin), P62804 (Rat), P62805 (Human), P62806 (Mouse)

Gene ID

121504 (HIST4H4), 8367 (HIST1H4E), 8365 (HIST1H4H), 8361 (HIST1H4F), 8362 (HIST1H4K), 8370 (HIST2H4A), 8359 (HIST1H4A), 319158 (Hist1h4i), 319159 (Hist1h4j), 319160 (Hist1h4k), 100041230 (Hist1h4m), 319161 (Hist1h4n), 97122 (Hist2h4), 320332 (Hist4h4), 69386 (Hist1h4h), 319156 (Hist1h4d), 319157 (Hist1h4f), 326620 (Hist1h4b), 319155 (Hist1h4c), 554313 (HIST2H4B), 8360 (HIST1H4D), 8368 (HIST1H4L), 64627, 8363 (HIST1H4J), 326619 (Hist1h4a), 8364 (HIST1H4C), 8366 (HIST1H4B), 295277

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mam (Mammalia), Amph (Amphibian), Dros (Drosophila), Ye (Yeast), Pl (Plant), Hst (Hamster), Chk (Chicken), Por (Porcine), Mky (Monkey), Can (Canine), GP (Guinea Pig), Gt (Goat), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Rat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ChIP assay (ChIP), Dot blot (Dot), Frozen Sections (C)

Background of Histone H4 antibody

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the D into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of D to form one octameric nucleosome. Histone tails undergo numerous post-translatiol modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptiol activation or repression or other nuclear processes. In addition to the genetic code, combitions of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dymically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H4 is associated with active gene transcription.

General readings

1. Turner, B.M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabelling with site-specific antibodies.
FEBS Letters 253: 141-145.
2. Turner, B.M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei. Cell 69:
375-384.
3. Belyaev, N.D. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells. Hum. Genet. 97: 573-578.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Histone H4 antibody

Western blot analysis using the Histone H4 ( K20me3 ) polyclonal antibody ( Cat # PAB0657 ).
Histone ( acid ) extracts of NB4 cells were analysed by Western blot using the Histone H4 ( K20me3 ) polyclonal antibody ( Cat # PAB0657 ) at a dilution of 1 : 750 in TBS-Tween + 5% skimmed milk. The location of the protein of interest is indicated. Rabbit IgG Purified Hu ChIP, Dot, E, WB
50 µl / €470.00
  Abnova Taiwan Corp.

Histone H4 antibody

Western blot analysis of Histone H4 expression with rat kidney tissue lysate. Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of Acetyl-Histone H4 phosphorylation expression in TSA treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of Histone H4 expression in TSA treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using MonoMethyl-Histone H4-K20 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using TriMethyl-Histone H4-K20 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using Asymmetric DiMethyl-Histone H4-R3 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using Symmetric DiMethyl-Histone H4-R3 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4K20me2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

Host: Rabbit; Target Name: HIST1H4A; Sample Tissue: 293T Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, GP, Gt, Ms, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB using the antibody against H4K20me1 diluted 1:750 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB using the antibody against H4K20me3, diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB using the antibody against H4K8ac diluted 1:250 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right. Lane 2 shows the result of the Western analysis with the antibody; lane 1 shows the same analysis after incubation of the antibody with 750 pmol blocking peptide for 1 hour at room temperature. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2 ) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5,8,12ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu, Ms E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

ChIP assays using HeLa cells (sheared chromatin from 1 million cells). Titration of 1, 2, 5 and 10ug antibody per ChIP was analysed. IgG (2 ug/IP) was used as negative control. qPCR primers were for promoter of active gene EIF4A2 and for a region 1 kb upstream of GAPDH as positive controls, and for inactive MYOD1 and the Sat2 satellite repeat region used as negative controls. Image shows the recovery, expressed as a % of input (the relative amount of IP'd DNA compared to input DNA after qPCR). Rabbit IgG Purified Hu, Ms E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2 ) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left. Rabbit IgG Purified Hu, Ms E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H4K20me1. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left, the position of the protein is indicated on the right. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB using the antibody against H4K20me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

WB using the antibody against H4K8ac diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. Rabbit IgG Purified Hu, Ms E
50 µg / €325.00
  OriGene Technologies, Inc.

Histone H4 antibody

ChIP assays using HeLa cells: ChIP” kit on sheared chromatin from 10,000 cells using the SX-8G IP-Star automated system. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the ACTB promoter and for the second exon of the MB gene. Image shows the recovery, expressed as a % of input (the relative amount of IP'd DNA compared to input DNA after qPCR analysis). Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

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