ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Flow Cytometry (F), EPair for Elisa (EPair), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of HLA class I A-3 alpha / HLA-A antibody
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq]
HLA-A MaxPab polyclonal antibody. Western Blot analysis of HLA-A expression in human spleen.
1X10^6 HepG2 cells were stained with 0.2ug HLA class I (HLA-A) antibody (15240-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:200.
Immunoprecipitation of HLA-A transfected lysate using anti-HLA-A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HLA-A purified MaxPab mouse polyclonal antibody (B01P) (H00003105-B01P).
HLA-A antibody [N1C2] immunoprecipitates HLA-A protein in IP experiments.IP samples: A549 whole cell extractA. Control with 4 μg of preimmune Rabbit IgGB. Immunoprecipitation of HLA-A protein by 4 μg HLA-A antibody [N1C2] (GTX114080)10 % SDS-PAGEThe immunoprecipitated HLA-A protein was detected by HLA-A antibody [N1C2] (GTX114080) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
1X10^6 HEK-293 cells were stained with .2ug HLA class I (HLA-A) antibody (66013-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
1X10^6 HepG2 cells were stained with .2ug HLA class I (HLA-A) antibody (66013-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.