HLA class I alpha G / HLA-G antibody

Principal name

HLA class I alpha G / HLA-G antibody

Alternative names for HLA class I alpha G / HLA-G antibody

HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen

SwissProt ID

P17693 (Human)

Gene ID

3135 (HLA-G)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), ELISA (capture) (E(capture))

Background of HLA class I alpha G / HLA-G antibody

The monoclol antibody 87G recognizes human HLA-G, a member of the Human Leukocyte Antigen family but as part of the nonclassical MHC type involved in inhibiting immune reponses. HLA-G has seven reported isoforms. The antibody 87G recognizes both HLA-G1 and the solube HLA-G5. Expression of HLA-G is found primarily in fetal trophoblast cells as they invade the materl decidua thereby protecting the fetus from the materl immune system. Like the highly mitotic trophoblast, abundant HLA-G protein expression has been identified in some tumors, including melanoma, breast carcinoma and rel carcinoma as well as CLL, AML and B-CLL. Some expression has also been found in pancreatic islets, erythroid and endothelial progenitors and the adult thymic medulla.HLA-G+ CD4 or CD8 cells have been identified in normal human peripheral blood and are thought to act as regulatory cells in that they are hypoproliferative with a unique cytokine profile differing from Tregs. The receptors for HLA-G are CD85j/ILT2, CD85d/ILT4, and CD158. Recent studies have shown a role for HLA-G in tolerance and maintence of transplanted organs.

General readings

Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N.: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;6 (3):315-26.
Boyson JE, Erskine R, Whitman MC, Chiu M, Lau JM, Koopman LA, Valter MM, Angelisova P, Horejsi V, Strominger JL.: Disulfide bond-mediated dimerization of HLA-G on the cell surface. Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16180-5.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HLA class I alpha G / HLA-G antibody

Western blot analysis of HLA-G Antibody (Center) (Cat. #TA325084) in NCI-H460 cell line lysates (35ug/lane). HLA-G (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HLA class I alpha G / HLA-G antibody

Staining of stimulated U937 cells with 0.25 ug of Mouse IgG2a K Isotype Control Purified (blue) or 0.25 ug of Anti-Human HLA-G Purified (purple) followed by F(ab')2 Anti-Mouse IgG PE .  Total viable cells were used for analysis. Mouse 87G Purified Hu F, P
0.1 mg / €120.00
  OriGene Technologies, Inc.

HLA class I alpha G / HLA-G antibody

HLA-G Antibody(Cat. #TA324985) western blot analysis in A549,Ramos,MDA-MB-231 cell line lysates (35ug/lane).This demonstrates the HLA-G antibody detected the HLA-G protein (arrow). Mouse IgG1 800CT6.4.2 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HLA class I alpha G / HLA-G antibody

HLA-G Antibody(Cat. #TA324987) western blot analysis in A549,Ramos,MDA-MB-231 cell line lysates (35ug/lane).This demonstrates the HLA-G antibody detected the HLA-G protein (arrow). Mouse IgG1 800CT6.4.2 Ascites Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HLA class I alpha G / HLA-G antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLA-G (RC205216, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLA-G. Mouse IgG1 OTI3H1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HLA class I alpha G / HLA-G antibody

Surface staining of HLA-G transfectants (red) compared with K562 cells (orange) and blank (blue), with anti-HLA-G antibody (87G) biotin / streptavidin-PE. Mouse IgG2a 87G Biotin Hu E, F
0.1 mg / €370.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

Surface staining of HLA-G transfectants (red) compared with K562 cells (orange) and blank (blue), with anti-HLA-G antibody (87G) biotin / streptavidin-PE. Mouse IgG2a 87G FITC Hu F
0.1 mg / €370.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

Surface staining of HLA-G transfectants (red) compared with K562 cells (orange) and blank (blue), with anti-HLA-G antibody (87G) biotin / streptavidin-PE. Mouse IgG2a 87G PE Hu F
0.1 mg / €430.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G (C-term) antibody

Western Blotting analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti--Human AM03081PU-N HLA-G antibody (5A6G7). 
Lane 1: M8 cell line transfected with empty vector. 
Lane 2: M8 cell line transfected with HLA-G1. Lane 3: M8 cell line transfected with HLA-G5. Lane 4: M8 cell line transfected with HLA-G6. Mouse IgG1 5A6G7 Purified Hu C, E, E(capture), F, ICC/IF, P, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G (C-term) antibody

HLA class I  alpha G / HLA-G Mouse IgG1 2A12 Purified Hu C, ICC/IF, P, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

Immunohistochemistry staining of kidney allograft biopsy (paraffin-embedded sections) with anti-human HLA-G (MEM-G/2). Mouse IgG1 MEM-G/2 Purified Hu P, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) purified, GAM-APC. Mouse IgG1 01G Purified Hu C, E, F, ICC/IF, IP
0.1 mg / €270.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

HLA class I  alpha G / HLA-G Mouse IgG2a G233 Aff - Purified Hu E, F, IP
0.1 mg / €290.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

HLA class I  alpha G / HLA-G Mouse IgG1 MEM-G/1 Purified Hu P, WB
0.2 mg / €610.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

Immunohistochemistry staining with anti-human HLA-G (MEM-G/1). 
Fig. 1A - pulmonary disseases (paraffin-embedded sections) The antibody MEM-G/1 stains infiltrating macrophages in pulmonary diseases. In the top left corner see the detail of macrophage.
Fig. 1B - first-trimester placenta (paraffin-embedded sections) Mouse IgG1 MEM-G/1 Biotin Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

Figure 2: first-trimester placenta (Paraffin-Embedded Sections) Mouse IgG1 MEM-G/1 Purified Hu P, WB
0.1 mg / €270.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC. Mouse IgG1 MEM-G/9 APC Hu F
0.1 mg / €430.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC. Mouse IgG1 MEM-G/9 Azide Free Hu C, E, F, ICC/IF, IP
0.1 mg / €270.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC. Mouse IgG1 MEM-G/9 Biotin Hu F
0.1 mg / €370.00
  Acris Antibodies GmbH

HLA class I alpha G / HLA-G antibody

Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC. Mouse IgG1 MEM-G/9 FITC Hu F
0.1 mg / €370.00
  Acris Antibodies GmbH

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