HMBS antibody

Principal name

HMBS antibody

Alternative names for HMBS antibody

PBGD, UPS, Porphobilinogen deaminase, Hydroxymethylbilane synthase, Pre-uroporphyrinogen synthase

SwissProt ID

P08397, P19356, P22907, Q2KIN5

Gene ID

3145 (HMBS)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), Rb (Rabbit), Sh (Sheep), GP (Guinea Pig), Gt (Goat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P)

Background of HMBS antibody

Kit Component:
- KN201362G1, HMBS gRNA vector 1 in pCas-Guide vector
- KN201362G2, HMBS gRNA vector 2 in pCas-Guide vector
- KN201362D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Schneider Yin X., et al. (2004) J Inherit Metab Dis. 625-631:471-474.Sheppard L., et al. (1995) Paediatr Anaesth. 15:426-428.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HMBS antibody

  HMBS mouse monoclonal antibody,clone UMAB144  
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min) Mouse IgG1 UMAB144 Purified Hu P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

HMBS antibody

  HMBS mouse monoclonal antibody,clone UMAB144  
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min) Mouse IgG1 UMAB144 Purified Hu P, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

HMBS antibody

  HMBS mouse monoclonal antibody, clone OTI3F4 (formerly 3F4)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HMBS mouse monoclonal antibody. (TA802669) Dilution: 1:150 Mouse IgG1 OTI3F4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HMBS antibody

  Anti-HMBS Rabbit Polyclonal Antibody  
Predicted band size: 39 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HMBS antibody

  Rabbit anti-HMBS Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using HMBS antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HMBS antibody

  Rabbit Polyclonal Anti-HMBS Antibody  
WB Suggested Anti-HMBS Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate Rabbit IgG Purified Can, Eq, Hu, Por, Rb, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

HMBS antibody

  Rabbit Polyclonal Anti-HMBS Antibody  
WB Suggested Anti-HMBS Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Liver Rabbit IgG Purified Can, GP, Gt, Hu, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

HMBS antibody

  HMBS mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS (RC201362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMBS. Mouse IgG1 OTI2F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HMBS antibody

  HMBS mouse monoclonal antibody, clone OTI1F1 (formerly 1F1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS (RC201362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMBS. Mouse IgG1 OTI1F1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HMBS antibody

  HMBS mouse monoclonal antibody, clone OTI5B11 (formerly 5B11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS (RC201362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMBS. Mouse IgG1 OTI5B11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HMBS antibody

  HMBS mouse monoclonal antibody, clone OTI3E2 (formerly 3E2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS (RC201362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMBS. Mouse IgG1 OTI3E2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

HMBS - human gene knockout kit via CRISPR

HMBS - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

HMBS - human gene knockout kit via CRISPR

HMBS - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

HMBS - human gene knockout kit via CRISPR

HMBS - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

HMBS - human gene knockout kit via CRISPR

HMBS - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

HMBS (transcript variant 1)

  Recombinant protein of human hydroxymethylbilane synthase (HMBS), transcript variant 1  
HMBS Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

HMBS (transcript variant 2)

  Recombinant protein of human hydroxymethylbilane synthase (HMBS), transcript variant 2  
HMBS Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

HMBS (1-361, His-tag)

HMBS Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

HMBS (1-361, His-tag)

HMBS Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

HMBS overexpression lysate

HMBS overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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