HMBS antibody

Principal name

HMBS antibody

Alternative names for HMBS antibody

PBGD, UPS, Porphobilinogen deaminase, Hydroxymethylbilane synthase, Pre-uroporphyrinogen synthase

SwissProt ID

P08397, P19356, P22907, Q2KIN5

Gene ID

3145 (HMBS)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), Rb (Rabbit), Sh (Sheep), GP (Guinea Pig), Gt (Goat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P)

Background of HMBS antibody

Kit Component:
- KN201362G1, HMBS gRNA vector 1 in pCas-Guide vector
- KN201362G2, HMBS gRNA vector 2 in pCas-Guide vector
- KN201362D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Schneider Yin X., et al. (2004) J Inherit Metab Dis. 625-631:471-474.Sheppard L., et al. (1995) Paediatr Anaesth. 15:426-428.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HMBS antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min) Mouse IgG1 UMAB144 Purified Hu P, WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

HMBS antibody

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min) Mouse IgG1 UMAB144 Purified Hu P, WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

HMBS antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HMBS mouse monoclonal antibody. (TA802669) Dilution: 1:150 Mouse IgG1 OTI3F4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HMBS antibody

Predicted band size: 39 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HMBS antibody

Western blot analysis of extracts of various cell lines, using HMBS antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HMBS antibody

WB Suggested Anti-HMBS Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate Rabbit IgG Purified Can, Eq, Hu, Por, Rb, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

HMBS antibody

WB Suggested Anti-HMBS Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Liver Rabbit IgG Purified Can, GP, Gt, Hu, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

HMBS antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS (RC201362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMBS. Mouse IgG1 OTI2F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HMBS antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS (RC201362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMBS. Mouse IgG1 OTI1F1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HMBS antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS (RC201362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMBS. Mouse IgG1 OTI5B11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HMBS antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMBS (RC201362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMBS. Mouse IgG1 OTI3E2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HMBS antibody

Western Blot analysis of HMBS expression in transfected 293T cell line (H00003145-T01) by HMBS MaxPab polyclonal antibody.

Lane 1: HMBS transfected lysate(39.71 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

HMBS antibody

Western Blot analysis of HMBS expression in transfected 293T cell line (H00003145-T01) by HMBS MaxPab polyclonal antibody.

Lane 1: HMBS transfected lysate(39.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

HMBS antibody

Western Blot analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.

Lane 1: HMBS transfected lysate(39.71 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 3E8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HMBS antibody

Detection limit for recombinant GST tagged HMBS is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 2B12 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

HMBS antibody

Human 721_B; WB Suggested Anti-HMBS Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate; HMBS antibody - N-terminal region (ARP54451_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

HMBS antibody

Human Liver; WB Suggested Anti-HMBS Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Liver; HMBS antibody - middle region (ARP54452_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

HMBS (transcript variant 1)

HMBS Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

HMBS (transcript variant 2)

HMBS Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

HMBS (1-361, His-tag)

HMBS Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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