HMG-CoA lyase / HMGCL antibody
Principal name
HMG-CoA lyase / HMGCL antibody
Alternative names for HMG-CoA lyase / HMGCL antibody
mitochondrial Hydroxymethylglutaryl-CoA lyase
Ncbi ID
9606, NP_000182, NP_000182.2, NM_001166059, NP_077362.1, NM_024386, NM_000191
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of HMG-CoA lyase / HMGCL antibody
HMGCL, also known as hydroxymethylglutaryl-CoA lyase, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It exists as a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies that, during fasting, provide a major source of energy for heart, brain and kidney. More specifically, it catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA.
General readings
Fu, Z., et al. J. Biol. Chem. 285(34):26341-26349(2010)
Pierron, S., et al. Arch Pediatr 17(1):10-13(2010)
Menao, S., et al. Hum. Mutat. 30 (3), E520-E529 (2009)
Lin, W.D., et al. Clin. Chim. Acta 401 (1-2), 33-36 (2009)
Carrasco, P., et al. Mol. Genet. Metab. 91(2):120-127(2007)
Western Blot analysis of HMGCL expression in transfected 293T cell line by HMGCL monoclonal antibody (M01), clone 4F4-D1.Lane 1: HMGCL transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.
HMGCL monoclonal antibody (M01), clone 4F4-D1 Western Blot analysis of HMGCL expression in A-431 ( Cat # L015V1 ).
Western Blot analysis of HMGCL expression in transfected 293T cell line (H00003155-T01) by HMGCL MaxPab polyclonal antibody.Lane 1: HMGCL transfected lysate(34.40 KDa).Lane 2: Non-transfected lysate.
Human Liver; WB Suggested Anti-HMGCL Antibody Titration: 1.25ug/ml. Positive Control: Human Liver; HMGCL antibody - N-terminal region (ARP41686_T100) in Human Liver cells using Western Blot
Human HepG2; WB Suggested Anti-HMGCL Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; HMGCL antibody - C-terminal region (ARP41687_P050) in Human HepG2 cells using Western Blot
Western blot analysis of HMG-CoA lyase / HMGCL Antibody (Center) Cat.-No AP52061PU-N in WiDr cell line lysates (35ug/lane). This demonstrates the HMGCL antibody detected the HMGCL protein (arrow).
HMGCL antibody detects HMGCL protein by Western blot analysis. A. 50 ug mouse liver lysate/extract. 10 % SDS-PAGE. HMGCL antibody (TA308677) dilution: 1:10000
Sample (30 ug of whole cell lysate). A: H1299. 10% SDS PAGE. TA308677 diluted at 1:3000
HMGCL antibody detects HMGCL protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 10 % SDS-PAGE. HMGCL antibody (TA308677) dilution: 1:10000
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HMGCL(TA308677) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HL(TA308677) antibody at 1:200 dilution.
Host: Rabbit; Target Name: Hmgcl; Sample Tissue: Rat Thymus lysates; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308677 | HMG-CoA lyase / HMGCL antibody |
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| Rabbit Polyclonal antibody to HMGCL (3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase) | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€416.00
|
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA336239 | HMG-CoA lyase / HMGCL antibody |
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| Rabbit Polyclonal Anti-Hmgcl Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350049 | HMG-CoA lyase / HMGCL antibody |
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| Rabbit Polyclonal Anti-HMGCL Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP52061PU-N | HMG-CoA lyase / HMGCL (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€370.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303817 | HMG-CoA lyase / HMGCL |
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| Recombinant protein of human 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase (HMGCL) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| AR50170PU-N | HMG-CoA lyase / HMGCL (28-325, His-tag) |
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Human | Purified | > 85 % by SDS - PAGE | E. coli |
0.5 mg /
€730.00
|
| OriGene Technologies GmbH | |||||
| AR50170PU-S | HMG-CoA lyase / HMGCL (28-325, His-tag) |
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|
Human | Purified | > 85 % by SDS - PAGE | E. coli |
0.1 mg /
€300.00
|
| OriGene Technologies GmbH | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY431216 | HMGCL overexpression lysate |
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC203817L2V | Lenti ORF particles, HMGCL (mGFP-tagged) - Human 3-hydroxymethyl-3-methylglutaryl-CoA lyase (HMGCL), nuclear gene encoding mitochondrial protein, transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€989.00
|
|
| OriGene Technologies, Inc. | ||
| RC203817L1V | Lenti ORF particles, HMGCL (Myc-DDK tagged) - Human 3-hydroxymethyl-3-methylglutaryl-CoA lyase (HMGCL), nuclear gene encoding mitochondrial protein, transcript variant 1 , 200 uL, >10^7 TU/mL |
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|
200 µl /
€886.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214875L2V | Lenti ORF particles, Hmgcl (GFP-tagged ORF) - Rat 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase (Hmgcl), 200 uL, >10^7 TU/mL |
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200 µl /
€907.00
|
|
| OriGene Technologies, Inc. | ||
| RR214875L1V | Lenti ORF particles, Hmgcl (Myc-DDK-tagged ORF) - Rat 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase (Hmgcl), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€804.00
|
|
| OriGene Technologies, Inc. | ||
