HMG-CoA lyase / HMGCL antibody
Principal name
HMG-CoA lyase / HMGCL antibody
Alternative names for HMG-CoA lyase / HMGCL antibody
mitochondrial Hydroxymethylglutaryl-CoA lyase
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of HMG-CoA lyase / HMGCL antibody
HMGCL, also known as hydroxymethylglutaryl-CoA lyase, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It exists as a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies that, during fasting, provide a major source of energy for heart, brain and kidney. More specifically, it catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA.
General readings
Fu, Z., et al. J. Biol. Chem. 285(34):26341-26349(2010)
Pierron, S., et al. Arch Pediatr 17(1):10-13(2010)
Menao, S., et al. Hum. Mutat. 30 (3), E520-E529 (2009)
Lin, W.D., et al. Clin. Chim. Acta 401 (1-2), 33-36 (2009)
Carrasco, P., et al. Mol. Genet. Metab. 91(2):120-127(2007)
Immunohistochemical of paraffin-embedded human liver using 16898-1-AP(HMGCL antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using 16898-1-AP(HMGCL antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human ovary using 16898-1-AP(HMGCL antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human ovary using 16898-1-AP(HMGCL antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using 16898-1-AP(HMGCL antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using 16898-1-AP(HMGCL antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using 16898-1-AP(HMGCL antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human testis using 16898-1-AP(HMGCL antibody) at dilution of 1:100 (under 40x lens)
Human heart tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP(HMGCL antibody) at dilution of 1:300
Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP(HMGCL antibody) at dilution of 1:600
Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16898-1-AP(HMGCL antibody) at dilution of 1:300
Western blot analysis of HMG-CoA lyase / HMGCL Antibody (Center) Cat.-No AP52061PU-N in WiDr cell line lysates (35ug/lane). This demonstrates the HMGCL antibody detected the HMGCL protein (arrow).
Human Liver; WB Suggested Anti-HMGCL Antibody Titration: 1.25ug/ml. Positive Control: Human Liver; HMGCL antibody - N-terminal region (ARP41686_T100) in Human Liver cells using Western Blot
Human HepG2; WB Suggested Anti-HMGCL Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; HMGCL antibody - C-terminal region (ARP41687_P050) in Human HepG2 cells using Western Blot
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HMGCL(GTX109096) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HL(GTX109096) antibody at 1:200 dilution.
HMGCL antibody detects HMGCL protein by Western blot analysis.A. 50 µg mouse liver lysate/extract10 % SDS-PAGEHMGCL antibody (GTX109096) dilution: 1:10000
HMGCL antibody detects HMGCL protein by Western blot analysis.A. 50 µg rat liver lysate/extract10 % SDS-PAGEHMGCL antibody (GTX109096) dilution: 1:10000
Sample (30 ug of whole cell lysate)A: H129910% SDS PAGEGTX109096 diluted at 1:3000
Immunohistochemical analysis of paraffin-embedded Breast ca, using HMGCL(GTX114472) antibody at 1:250 dilution.
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE HL antibody GTX114472 diluted at 1:1000
Western Blot analysis of HMGCL expression in transfected 293T cell line (H00003155-T01) by HMGCL MaxPab polyclonal antibody.Lane 1: HMGCL transfected lysate(34.40 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of HMGCL expression in transfected 293T cell line by HMGCL monoclonal antibody (M01), clone 4F4-D1.Lane 1: HMGCL transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.
HMGCL monoclonal antibody (M01), clone 4F4-D1 Western Blot analysis of HMGCL expression in A-431 ( Cat # L015V1 ).
Western Blot: HMGCL Lysate [NBL1-11617] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for HMGCL
Western Blot: HMGCL Antibody [NBP1-58026] - This Anti-HMGCL antibody was used in Western Blot of fetal liver tissue lysate at a concentration of 1.25ug/ml.
Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Western Blot: HMGCL Antibody [NBP1-83251] Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
10ug of GTX109096-pro HL recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308677 | HMG-CoA lyase / HMGCL antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA336239 | HMG-CoA lyase / HMGCL antibody |
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Rabbit | IgG | Purified | Hu, Rt | WB |
50 µg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| AP52061PU-N | HMG-CoA lyase / HMGCL (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€370.00
|
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| Acris Antibodies GmbH | |||||||
| H00003155-A01 | HMG-CoA lyase / HMGCL antibody |
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Mouse | Serum | Hu | E |
50 µl /
€290.00
|
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| Abnova Taiwan Corp. | |||||||
| H00003155-D01 | HMG-CoA lyase / HMGCL antibody |
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Rabbit | Serum | Hu | E(detection), IP, WB |
0.1 ml /
€380.00
|
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| Abnova Taiwan Corp. | |||||||
| H00003155-D01P | HMG-CoA lyase / HMGCL antibody |
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 |
Rabbit | Purified | Hu | E(detection), WB |
0.1 mg /
€380.00
|
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| Abnova Taiwan Corp. | |||||||
| H00003155-M01 | HMG-CoA lyase / HMGCL antibody |
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 |
Mouse | IgG2a | 4F4-D1 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| ARP41686_T100 | HMG-CoA lyase / HMGCL antibody |
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 |
Rabbit | Aff - Purified | Bov, Can, Dros, Hu, Ms, Rt, Ze | WB |
0.1 mg /
€310.00
|
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| AVIVA Systems Biology | |||||||
| ARP41687_P050 | HMG-CoA lyase / HMGCL antibody |
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 |
Rabbit | Aff - Purified | Bov, Can, Chk, Hu, Ms, Rt, Ze | WB |
50 µg /
€350.00
|
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| AVIVA Systems Biology | |||||||
| NBP1-32767 | HMG-CoA lyase / HMGCL antibody |
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Rabbit | IgG | Hu | ICC/IF, P, WB |
0.1 mg /
€420.00
|
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| Novus Biologicals Inc. | |||||||
| NBP1-58026 | HMG-CoA lyase / HMGCL antibody |
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![Western Blot: HMGCL Antibody [NBP1-58026] - This Anti-HMGCL antibody was used in Western Blot of fetal liver tissue lysate at a concentration of 1.25ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/HMGCL Antibody-Western Blot-NBP1-58026-img0001.jpg) |
Rabbit | Purified | Bov, Can, Dros, Hu, Ms, Rt, Ze | WB |
0.1 mg /
€360.00
|
||
| Novus Biologicals Inc. | |||||||
| NBP1-83251 | HMG-CoA lyase / HMGCL antibody |
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![Immunohistochemistry-Paraffin: HMGCL Antibody [NBP1-83251] - Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/HMGCL Antibody-Immunohistochemistry-Paraffin-NBP1-83251-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303817 | HMG-CoA lyase / HMGCL |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| AR50170PU-N | HMG-CoA lyase / HMGCL (28-325, His-tag) |
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Human | Purified | > 85 % by SDS - PAGE | E. coli |
0.5 mg /
€730.00
|
| Acris Antibodies GmbH | |||||
| AR50170PU-S | HMG-CoA lyase / HMGCL (28-325, His-tag) |
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|
Human | Purified | > 85 % by SDS - PAGE | E. coli |
0.1 mg /
€300.00
|
| Acris Antibodies GmbH | |||||
| H00003155-P01-10 | HMG-CoA lyase / HMGCL |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00003155-P01-25 | HMG-CoA lyase / HMGCL |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
Antibody Pairs
| Catalog No. | ||
|---|---|---|
| H00003155-PW1 | HMGCL (Human) IP-WB Antibody Pair |
|
|
1 set /
€710.00
|
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| Abnova Taiwan Corp. | ||
| H00003155-AP51 | HMGCL (Human) Matched Antibody Pair |
|
|
1 set /
€710.00
|
|
| Abnova Taiwan Corp. |
Lysates
| Catalog No. | ||
|---|---|---|
| LY431216 | HMGCL overexpression lysate |
|
|
0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
