HMGB1 antibody

Principal name

HMGB1 antibody

Alternative names for HMGB1 antibody

High mobility group protein 1, HMG1, HMG-1, High mobility group protein B1, HMGB-1, Amphoterin

SwissProt ID

P09429 (Human), P10103 (Bovin), P12682 (Pig), P63158 (Mouse), P63159 (Rat), Q6NX86, Q6YKA4 (Canfa)

Gene ID

3146 (HMGB1), 4321622 (Q0CJD8), 15289 (Hmgb1)

Available reactivities

Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Eq (Equine), Rb (Rabbit), Sh (Sheep), African clawed frog, Ze (Zebrafish), Mam (Mammalia)

Available hosts

Mouse, Rabbit, Hamster

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ChIP assay (ChIP), Flow Cytometry (F), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection))

Background of HMGB1 antibody

High mobility group protein B1 (HMGB1) belongs to a family of highly conserved proteins that contain HMG box domains (1,2). All three family members (HMGB1, HMGB2, and HMGB3) contain two HMG box domains and a C-termil acidic domain. HMGB1 is a widely expressed and highly abundant protein. HMGB2 is widely expressed during embryonic development, but is restricted to lymphoid organs and testis in adult animals. HMGB3 is only expressed during embryogenesis. While expression varies, the biochemical properties of the different family members may be indistinguishable. The HMG box domains facilitate the binding of HMGB proteins to the minor groove of D, which results in local bending of the D double helix. HMGB proteins are recruited by and help facilitate the assembly of site-specific D binding proteins to their cogte binding sites in chromatin. For example, HMGB1 facilitates the binding of Hox proteins, Oct-1, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters. In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular sigling associated with inflammation. HMGB1 is massively released into the extracellular environment during cell necrosis, but not apoptosis. Extracellular HMGB1 "alarms" the inte immune system by acting as a chemoattractant for inflammatory leukocytes, smooth muscle cells, and stem cells, functioning as an immune adjuvant for soluble and particulate antigens, and triggering activation of T cells and dendritic cells. In addition, activated monocytes, macrophages and, dendritic cells also secrete HMGB1, forming a positive feedback loop that results in the release of additiol cytokines and neutrophils. Hypoxia has also been shown to cause the release of HMGB1 in the liver, and some studies suggest a role for extracellular HMGB1 in tumor homeostasis.

General readings

Ichiaki Ito et al. Post-translational Methylation of High Mobility Group Box 1 (HMGB1) Causes Its ytoplasmic Localization in Neutrophils.June 1, 2007 The Journal of Biological Chemistry, 282, 16336-16344.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HMGB1 antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1D5 Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

HMGB1 antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1B11 Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

HMGB1 antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2F6 Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

HMGB1 antibody

Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. [antibody concentration 3ug/ml] Mouse IgG2a 1D5 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HMGB1 antibody

Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml] Mouse IgG2a 1B11 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HMGB1 antibody

Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG2a 2F6 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HMGB1 antibody

Detection of HMGB1 in whole cell HeLa lysate. ECL exposure, 1 min Rabbit IgG Purified Bov, Can, Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HMGB1 antibody

Western blot analysis of extracts from Jurkat cells, HepG2 cells, COLO205 cells and 293 cells, using HMGB1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HMGB1 antibody

Anti-HMG1 / HMGB1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml. Mouse IgG2b IMG19N10B7 Purified Hu, Ms P
50 µg / €345.00
  OriGene Technologies, Inc.

HMGB1 antibody

Predicted cell location: Nucleus. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using HMGB1 antibody at dilution 1/13, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HMGB1 antibody

Western blot analysis of various cell lines, using HMGB1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HMGB1 antibody

Western blot analysis of HMGB1 expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HMGB1 antibody

Western Blot: HMGB1 Antibody (1F3) [TA337101] - Blot of crude HeLa cell extract. HMGB1 runs at an apparent molecular weight of 25kDa. Mouse IgG2b 1F3 Purified Bov, Hu, Ms, Por, Rt F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HMGB1 antibody

Simple Western: HMGB1 Antibody (19N12A1) [TA337171] - Simple Western lane view shows a specific band detected for HMGB1 in HeLa lysate.  This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66- Mouse 19N12A1 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HMGB1 antibody

Western blot analysis of HMGB1 in human brain tissue  lysate with HMGB1 antibody at (A) 1 and (B) 2 ug/mL Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HMGB1 antibody

Immunohistochemistry: Paraffin embedded sections of human colon cancer tissue were incubated with anti-human HMGB1 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate bufferand detected using Diaminobenzidine (DAB). Mouse IgG2b J2E1 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

HMGB1 antibody

Immunohistochemistry: Paraffin embedded sections of human colon cancer tissue were incubated with anti-human HMGB1 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate bufferand detected using Diaminobenzidine (DAB). Mouse IgG2b J2E1 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

HMGB1 antibody

Western blot analysis of HMGB1 expression in Raji (1), HL-60 (2), WR19L (3) and PC12 (4) using AM26447AF-N. Mouse IgG2a KS1 Azide Free Hu, Ms, Por, Rt WB
0.1 mg / €380.00
  Acris Antibodies GmbH

HMGB1 antibody

Western blot analysis of HMGB1 expression in Raji (1), HeLa (2), HL-60 (3), WR19L (4) and Rat-1 (5) using AM26643AF-N. Mouse IgG 4C9 Azide Free Hu, Ms, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

HMGB1 (C-term) antibody

Hela cells were stained with anti-HMGB1 Antibody Cat.-No AM33095PU (Clone 3E8), followed by DyLight™ 549 conjugated anti-mouse IgG. Nuclei were stained with DAPI. Mouse IgG2b 3E8 Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

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