HMGN1 antibody

Principal name

HMGN1 antibody

Alternative names for HMGN1 antibody

HMG14, HMG-14, Non-histone chromosomal protein HMG-14, High mobility group nucleosome-binding domain-containing protein 1

SwissProt ID

P02316 (Bovin), P05114 (Human), P12274 (Chick), P18608 (Mouse)

Gene ID

3150 (HMGN1)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Frog, Hu (Human), Mky (Monkey), Ms (Mouse), Por (Porcine), Rt (Rat), Rb (Rabbit), Dros (Drosophila), Orang-Utan, Xen (Xenopus), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of HMGN1 antibody

HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species. As such, this reagent is designed as a universal reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin.
HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family. Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the "HMG-box;" that of the HMGN family, the "nucleosomal binding domain;" and that of the HMGA family, the "AT-hook." The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as "HMG motif proteins."

General readings

Zhu,N. (2007) Mol. Cell. Biol. 27 (24), 8859-8873

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HMGN1 antibody

WB Suggested Anti-HMGN1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 721_B cell lysateHMGN1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HMGN1 antibody

WB Suggested Anti-HMGN1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

HMGN1 pSer20/24 (19-28) antibody

Formalin-Fixed Paraffin-Embedded Human Skin stained with Anti-HMGN1 antibody at 20 µg/ml after heat-induced antigen retrieval. Rabbit Aff - Purified Bov, Can, Chk, Frog, Hu, Mky, Ms, Por, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

HMGN1 pSer20/24 antibody

Immunohistochemistry of HMGN1 (phospho S20/24) polyclonal antibody ( Cat # PAB10016 ) was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA. Rabbit Bov, Chk, Hu, Ms, Rt E, P, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

HMGN1 pSer21 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
50 µg / €205.00
  Assay Biotech

HMGN1 pSer21 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HMG14 (Phospho-Ser21) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
0.1 mg / €310.00
  Assay Biotech

HMGN1 antibody

Immunofluorescence analysis of HepG2 cells, using HMG14 (Ab-21) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €205.00
  Assay Biotech

HMGN1 antibody

Immunofluorescence analysis of HepG2 cells, using HMG14 (Ab-21) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

HMGN1 antibody

Human Lung; . Rabbit Anti-HMGN1 Antibody. . Catalog Number:. ARP32627 . Paraffin Embedded Tissue: Human alveolar cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X Rabbit Aff - Purified Bov, Can, Hu, Ms, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

HMGN1 antibody

Human heart; WB Suggested Anti-HMGN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; HMGN1 antibody - middle region (ARP38532_P050) in Human heart cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

HMGN1 antibody

Western blot analysis of HMGN1 expression in Hela (A); NIH3T3 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

HMGN1 antibody

Western blot analysis of HMGN1 expression in Hela (A); NIH3T3 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

HMGN1 antibody

Western blot analysis of HMGN1 expression in Hela (A); NIH3T3 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

HMGN1 antibody

HMGN1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €360.00
  Novus Biologicals Inc.

HMGN1 antibody

HMGN1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €290.00
  Novus Biologicals Inc.

HMGN1 antibody

Western Blot: Histone H2Av  [p Ser137] Antibody [NBP1-78103] shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head).   Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose.  After blocking the membrane was probed with the primary antibody diluted to 1:500.  Washes and reaction with secondary antibody followed incubation. Rabbit Aff - Purified Dros E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

HMGN1 / HMGN2 pSer20/24 antibody

WB using Anti-HMNG antibody shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain: 1 - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. The primary antibody was diluted 1:1,000. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, Orang-Utan, Por, Rt, Xen E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

HMGN1

HMGN1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

HMGN1 (1-100, His-tag)

HMGN1 Human Purified > 95 % by SDS - PAGE E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

HMGN1 (1-100, His-tag)

HMGN1 Human Purified > 95 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

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