HNF1 alpha / TCF1 antibody

Principal name

HNF1 alpha / TCF1 antibody

Alternative names for HNF1 alpha / TCF1 antibody

HNF1A, LFB1, LF-B1, TCF-1, Transcription factor 1, Hepatocyte nuclear factor 1-alpha, Liver-specific transcription factor LF-B1

SwissProt ID

P15257 (Rat), P20823 (Human), P22361 (Mouse), Q90867 (Chick)

Gene ID

6927 (HNF1A)

Available reactivities

Hst (Hamster), Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus), Chk (Chicken), Por (Porcine), Can (Canine), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of HNF1 alpha / TCF1 antibody

T-cell factor-1 (TCF-1) is a DNA-binding transcriptional activator that isessential for lymphoid cell development. The TCF family of transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. Several alternative splice variants of TCF-1 have been identified, including TCF-1A, which share a conserved amino terminus and differ in the carboxy terminal sequences. The Wnt mediated signaling pathway induces cytosolic β-catenin binding to TCF proteins within the nucleus, leading to the enhanced expression of the Wnt target genes. The β-catenin-TCF complexes are negatively regulated by the adenomatous polyposis coli (APC) tumor suppressor protein, which phosphorylates β-catenin and, in turn, increases the degradation of cytosolic β-catenin and inhibits the transcriptional activity of the TCF proteins. Mutations in the APC gene, which are commonly observed in colorectal carcinomas, disrupt this regulatory pathway and correlate with an accumulation of β-catenin and the increased activation of the TCF target genes.

General readings

General / background references:Staal FJ et al. Tcf-1-mediated transcription in T lymphocytes: differential role for glycogen synthase kinase-3 in fibroblasts and T cells. Int Immunol 11:317-23 (1999). PubMed PMID: 10221643
Mayer K et al. Ectopic activation of lymphoid high mobility group-box transcription factor TCF-1 and overexpression in colorectal cancer cells. Int J Cancer 72:625-30 (1997). PubMed PMID: 9259402
Castrop J et al. The human TCF-1 gene encodes a nuclear DNA-binding protein uniquely expressed in normal and neoplastic T-lineage lymphocytes. Blood 86:3050-9 (1995). PubMed PMID: 7579399

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HNF1 alpha / TCF1 antibody

Figure 1. Western blot analysis using AM05273PU-N antibody at 1 µg/ml on Jurkat cell lysates. Mouse IgG 7H3 Purified Hu C, WB
0.1 mg / €540.00
  Acris Antibodies GmbH

HNF1 alpha / TCF1 antibody

WB Suggested Anti-HNF1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: OVCAR-3 cell lysate HNF1A is supported by BioGPS gene expression data to be expressed in OVCAR3 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HNF1 alpha / TCF1 antibody

Human kidney; HNF1A antibody - N-terminal region (ARP51375_P050) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified Chk, Hu, Ms, Por, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

HNF1 alpha / TCF1 antibody

Human HepG2; Host: Rabbit . Target Name: HNF1A . Sample Tissue: HepG2 . Antibody Dilution: 1.0ug/ml.; HNF1A antibody - N-terminal region (ARP57904_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

HNF1 alpha / TCF1 antibody

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein by Western blot analysis. A. 30 ug HepG2 whole cell lysate/extract. 7.5 % SDS-PAGE. HNF1 alpha antibody [N1N3] (TA308567) dilution: 1:5000 Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HNF1 alpha / TCF1 antibody

Western blot analysis of Human liver cancer tissue using TA321150 (HNF1A Antibody) at dilution 1/150 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HNF1 alpha / TCF1 antibody

Western blot analysis of Human liver cancer tissue using TA321151(HNF1A Antibody) at dilution 1/150 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HNF1 alpha / TCF1 antibody

Predicted band size: 56 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 45 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HNF1 alpha / TCF1 antibody

Western blot analysis of HNF1A Antibody (Center) (Cat. #TA325066) in mouse liver tissue lysates (35ug/lane). HNF1A (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HNF1 alpha / TCF1 antibody

HNF1 alpha / TCF1 Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

HNF1 alpha / TCF1 antibody

Western blot (WB) analysis of TCF-1 antibody (Cat.-No.: AP06685PU-N) in extracts from HUVEC cells. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

HNF1 alpha / TCF1 antibody

HNF1 alpha / TCF1 Mouse IgG 7H3 Hu C, WB
0.1 mg / €640.00
  Abnova Taiwan Corp.

HNF1 alpha / TCF1 antibody

HNF1 alpha / TCF1 Goat Hu, Ms, Rt E
0.1 mg / €390.00
  Abnova Taiwan Corp.

HNF1 alpha / TCF1 antibody

Western Blot: HNF1 Antibody (7H3) [NB120-12064] - Jurkat cell lysate probed with anti-TCF-1 at 1µg/ml. Mouse IgG 7H3 Hu C, P, WB
25 µg / €340.00
  Novus Biologicals Inc.

HNF1 alpha / TCF1 antibody

Immunohistochemistry: HNF1 Antibody [NBP1-33596] - Immunohistochemical analysis of HNF1 on paraffin-embedded A549 xenograft, using NBP1-33596. Rabbit IgG Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

HNF1 alpha / TCF1

HNF1 alpha / TCF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

HNF1 alpha / TCF1

HNF1 alpha / TCF1 Human
10 µg / €380.00
  Abnova Taiwan Corp.

HNF1 alpha / TCF1

HNF1 alpha / TCF1 Human
25 µg / €510.00
  Abnova Taiwan Corp.

18 Item(s)

per page
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