HNF4 alpha / TCF14 antibody

Principal name

HNF4 alpha / TCF14 antibody

Alternative names for HNF4 alpha / TCF14 antibody

HNF4A, HNF4, NR2A1, Hepatocyte nuclear factor 4-alpha, Transcription factor HNF-4, Transcription factor 14, Nuclear receptor subfamily 2 group A member 1

SwissProt ID

P22449 (Rat), P41235 (Human), P49698 (Mouse)

Gene ID

3172 (HNF4A), 25735 (Hnf4a), 3174 (HNF4G)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Electrophoretic Mobility Shift Assay (EMSA), Immunohistochemistry (IHC)

Background of HNF4 alpha / TCF14 antibody

Hepatocyte nuclear factor 4 alpha (HNF4 alpha), a NR2 Hepatocyte NF4-Like receptor, is a liver-enriched transcription factor that is involved in the expression of liver-specific genes. HNF4 alpha controls the expression of transcription factor HNF1, which is important in the expression of hepatic genes, including those involved in glucose, cholesterol, and fatty acid metabolism. HNF4 alpha is essential for hepatocyte differentiation during liver development, metabolic regulation, and liver function. Although HNF4 alpha is a tissue-specific transcription factor, it is also an essential regulator of early embryonic events. Mutations in the HNF4 alpha gene are responsible for type-1 maturity-onset diabetes of the young (MODY1), which is characterized by a defect in insulin secretion. Mutations in HNF4 alpha binding sites have been linked to hemophilia B Leyden, atherosclerosis, hepatitis, and other diseases. HNF4 alpha has been linked to liver cancer via hepatitis and hepatitis B viral infections. Mouse embryos lacking HNF4 alpha fail to complete gastrulation due to dysfunction of the visceral endoderm. Recently, it has been shown that HNF4 alpha is critically involved in the PXR- and CAR-mediated transcriptional activation of CYP3A4, a gene involved in the metabolism of a wide range of drugs Tirona et al. 2003. At least four isoforms (HNF4 alpha1/HNF4B, HNF4 alpha2/HNF4A, HNF4 alpha3/HNF4C, and HNF4 alpha4) are produced by alternative splicing.

General readings

Koh, W., et al. BMC Genomics 11 SUPPL 1, S6 (2010)
Wang, F., et al. Chin. Med. J. 122(20):2477-2482(2009)
Taghavi, S.M., et al. Cardiovasc Diabetol 8, 63 (2009)
Guo, H., et al. Biochem. J. 394 (PT 2), 379-387 (2006)
Hong, Y.H., et al. J. Biol. Chem. 278(30):27495-27501(2003)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HNF4 alpha / TCF14 antibody

Western blot (WB) analysis of HNF4α antibody (Cat.-No AP01345PU-N) in extracts from HT-29 cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

HNF4 alpha / TCF14 antibody

Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 4E2 Purified Hu E, ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

HNF4 alpha / TCF14 antibody

Hum. Fetal Heart; Host: Rabbit . Target Name: HNF4A . Sample Tissue: Human Fetal Heart . Antibody Dilution: 1.0ug/ml.; HNF4A Antibody - N-terminal region (ARP31946_P050) in Hum. Fetal Heart cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

HNF4 alpha / TCF14 (N-term) antibody

TA303011 (0.1µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HNF4 alpha / TCF14 antibody

Predicted band size: 52 kDa . Positive control: Mouse liver and Kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Mouse liver tissue lysate Lane2: Mouse Kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HNF4 alpha / TCF14 (Center) antibody

HNF4A Antibody (Center) (Cat. #TA324733) western blot analysis in 293,HepG2 cell line and rat liver tissue lysates (35ug/lane).This demonstrates the HNF4A antibody detected the HNF4A protein (arrow). Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HNF4 alpha / TCF14 antibody

Western blot analysis of HNF4 alpha expression in HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HNF4 alpha / TCF14 antibody

Western blot analysis of extracts of various cell lines, using HNF4A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HNF4 alpha / TCF14 antibody

Host: Rabbit; Target Name: HNF4A; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

HNF4 alpha / TCF14 antibody

WB Suggested Anti-HNF4A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: OVCAR-3 cell lysateHNF4A is strongly supported by BioGPS gene expression data to be expressed in Human OVCAR3 cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HNF4 alpha / TCF14 antibody

WB Suggested Anti-HNF4A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

HNF4 alpha / TCF14 antibody

Western blot analysis on HepG2 cell lysate using HNF4alpha /gamma Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HNF4 alpha / TCF14 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF4A (RC214914, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF4A. Mouse IgG2a OTI8B8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HNF4 alpha / TCF14 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HNF4A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806582) Mouse IgG1 OTI2H2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HNF4 alpha / TCF14 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF4A (RC214914, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF4A. Mouse IgG1 OTI9H8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HNF4 alpha / TCF14 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF4A (RC214914, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF4A. Mouse IgG1 OTI5A9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HNF4 alpha / TCF14 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF4A (RC214914, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF4A. Mouse IgG1 OTI2C10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HNF4 alpha / TCF14 Control Peptide

HNF4 alpha / TCF14 Purified
50 µg / €190.00
  Acris Antibodies GmbH

HNF4 alpha / TCF14 antibody

Western blot analysis of HNF-4 with mouse small intestine tissue lysate. Rabbit Aff - Purified Ms, Rt WB
0.1 mg / €370.00
  Acris Antibodies GmbH

HNF4 alpha / TCF14 (+ gamma) antibody

Immunohistochemistry (IHC) analysis of HNF-4α/γ antibody (Cat.-No.: AP06689PU-N) on paraffin-embedded sections. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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