hnRNP-K / HNRNPK antibody

Principal name

hnRNP-K / HNRNPK antibody

Alternative names for hnRNP-K / HNRNPK antibody

HNRPK, Heterogeneous nuclear ribonucleoprotein K, TUNP, Transformation up-regulated nuclear protein

SwissProt ID

O19049 (Rabit), P61978 (Human), P61979 (Mouse), P61980 (Rat), Q3T0D0 (Bovin), Q5ZIQ3 (Chick)

Gene ID

3190 (HNRNPK), 373190 (CyI), 528135

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Hst (Hamster), Xen (Xenopus), Eq (Equine), GP (Guinea Pig), Gt (Goat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection)), Dot blot (Dot), ChIP assay (ChIP)

Background of hnRNP-K / HNRNPK antibody

HNRPK belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Multiple alternatively spliced transcript variants have been described for this gene but only three variants have been fully described.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Multiple alternatively spliced transcript variants have been described for this gene but only three variants have been fully described.

General readings

Shnyreva M, Schullery DS, et al. (2000). J Biol Chem. 275(20):15498-503.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

hnRNP-K / HNRNPK antibody

Western blot analysis of extracts of various cell lines, using HNRNPK antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

hnRNP-K / HNRNPK antibody

Predicted band size: 51 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

hnRNP-K / HNRNPK antibody

HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-hnRNPK antibody (clone F45P9C7). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 F45P9C7 Aff - Purified Chk, Hu, Ms, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

hnRNP-K / HNRNPK antibody

WB Suggested Anti-HNRPK Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

hnRNP-K / HNRNPK antibody

WB Suggested Anti-HNRPK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

hnRNP-K / HNRNPK antibody

AM05384PU-N hnRNP-K antibody staining of Formalin-Fixed, Paraffin-Embedded Normal Colon (Left) and Colon
Carninoma (Right). Mouse IgG1 F45 P9 C7 Purified Chk, Hu, Ms, Rb, Rt C, WB
0.2 mg / €340.00
  Acris Antibodies GmbH

hnRNP-K / HNRNPK antibody

HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-hnRNPK antibody (clone F45P9C7). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 F45P9C7 Aff - Purified Chk, Hu, Ms, Rb, Rt P, WB
25 µg / €220.00
  Acris Antibodies GmbH

hnRNP-K / HNRNPK antibody

Western blot (WB) analysis of hnRNP K antibody (Cat.-No.: AP01359PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

hnRNP-K / HNRNPK (N-term) antibody

(LEFT) Western blot analysis of anti-HNRPK Antibody (N-term) (Cat.#AP12520PU-N) in 293 cell line lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-HNRPK Antibody (N-term) (Cat.#AP12520PU-N) in mouse brain tissue lysates (35ug/lane). HNRPK(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

hnRNP-K / HNRNPK antibody

Immunofluorescence of purified MaxPab antibody to HNRPK on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

hnRNP-K / HNRNPK antibody

Western Blot analysis of HNRNPK expression in transfected 293T cell line (H00003190-T02) by HNRNPK MaxPab polyclonal antibody.

Lane 1: HNRPK transfected lysate(51.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

hnRNP-K / HNRNPK antibody

Western blot using HNRNPK monoclonal antibody, clone F45 P9 C7 ( Cat # MAB1891 ) on Calu-6 ( 1 ) and HT-29 ( 2 ) cell lysates. Mouse IgG1 F45 P9 C7 Chk, Hu, Ms, Rb, Rt P, WB
0.2 mg / €390.00
  Abnova Taiwan Corp.

hnRNP-K / HNRNPK antibody

Western blot for HNRNPK monoclonal antibody, clone 3C2 ( Cat # MAB2478 ) on HeLa cell extracts. Mouse IgG2b 3C2 Bov, Hst, Hu, Rt E, ICC/IF, IP, WB
0.1 ml / €560.00
  Abnova Taiwan Corp.

hnRNP-K / HNRNPK pSer284 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with EGF 200ng/ml 30', using hnRNP K (Phospho-Ser284) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

hnRNP-K / HNRNPK pSer284 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with EGF 200ng/ml 30', using hnRNP K (Phospho-Ser284) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

hnRNP-K / HNRNPK antibody

Western blot analysis of extracts from K562/A549/HT-29 cells, using hnRNP K (Ab-284) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

hnRNP-K / HNRNPK antibody

Western blot analysis of extracts from K562/A549/HT-29 cells, using hnRNP K (Ab-284) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

hnRNP-K / HNRNPK antibody

Human Muscle; Rabbit Anti-HNRPK Antibody. Catalog Number: ARP40387. Paraffin Embedded Tissue: Human Muscle. Cellular Data: Skeletal muscle cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; HNRPK antibody - C-terminal region (ARP40387_T100) in Human Muscle cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rb, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

hnRNP-K / HNRNPK antibody

Human Kidney; Rabbit Anti-HNRPK Antibody. Catalog Number: ARP40388. Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; HNRPK antibody - N-terminal region (ARP40388_P050) in Human Kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rb, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

hnRNP-K / HNRNPK pSer284 antibody

Immunofluorescent analysis of hnRNP K (pS284) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rb, Rt, Ze ICC/IF, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

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