Homeobox protein goosecoid / GSC antibody
Principal name
Homeobox protein goosecoid / GSC antibody
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Homeobox protein goosecoid / GSC antibody
- Kit Component:
- - KN307365G1, Gsc gRNA vector 1 in pCas-Guide vector
- KN307365G2, Gsc gRNA vector 2 in pCas-Guide vector
- KN307365D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kohler,A., (2008) Am. J. Med. Genet. A 146 (1), 117-123
Immunoperoxidase of monoclonal antibody to GSC on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged GSC is approximately 0.1ng/ml as a capture antibody.
Western blot analysis of GSC over-expressed 293 cell line, cotransfected with GSC Validated Chimera RNAi ( Cat # H00145258-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GSC monoclonal antibody (M03), clone 3D11 (Cat # H00145258-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
GSC monoclonal antibody (M03), clone 3D11 Western Blot analysis of GSC expression in COLO 320 HSR ( Cat # L020V1 ).
Western Blot analysis of GSC expression in transfected 293T cell line by GSC monoclonal antibody (M03), clone 3D11.Lane 1: GSC transfected lysate(28.1 KDa).Lane 2: Non-transfected lysate.
GSC monoclonal antibody (M03), clone 3D11. Western Blot analysis of GSC expression in SJCRH30 ( Cat # L027V1 ).
Detection limit for recombinant GST tagged GSC is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GSC is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GSC is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GSC is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GSC is approximately 0.3ng/ml as a capture antibody.
Detection limit for recombinant GST tagged GSC is approximately 0.3ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to GSC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to GSC on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
GSC monoclonal antibody (M17), clone 2C1. Western Blot analysis of GSC expression in human kidney.
Immunoperoxidase of monoclonal antibody to GSC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
Western Blot analysis of GSC expression in transfected 293T cell line (H00145258-T01) by GSC MaxPab polyclonal antibody.Lane 1: GSC transfected lysate(28.27 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of GSC expression in transfected 293T cell line (H00145258-T01) by GSC MaxPab polyclonal antibody.Lane 1: GSC transfected lysate(28.10 KDa).Lane 2: Non-transfected lysate.
Human Tonsil; Immunohistochemistry with Human Tonsil lysate tissue at an antibody concentration of 5.0ug/ml using anti-GSC antibody (ARP32174_P050); GSC antibody - N-terminal region (ARP32174_P050) in Human Tonsil cells using Immunohistochemistry
Human Testis; Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-GSC antibody (ARP32174_P050); GSC antibody - N-terminal region (ARP32174_P050) in Human Testis cells using Immunohistochemistry
Human 786-0; Host: Rabbit. Target Name: GSC. Sample Tissue: 786-0 Whole Cell lysates. Antibody Dilution: 1.0ug/ml.; GSC antibody - N-terminal region (ARP32174_P050) in Human 786-0 cells using Western Blot
Human 721_B; WB Suggested Anti-GSC Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 721_B cell lysate; GSC antibody - N-terminal region (ARP32174_P050) in Human 721_B cells using Western Blot
Western Blot analysis of GSC expression in transfected 293T cell line (H00145258-T01) by GSC MaxPab polyclonal antibody.Lane 1: GSC transfected lysate(28.10 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of GSC transfected lysate using anti-GSC MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GSC MaxPab mouse polyclonal antibody (B01) (H00145258-B01).
Human Skin: Formalin-Fixed, Paraffin-Embedded
GSC monoclonal antibody (M17), clone 2C1. Western blot of GSC expression in human kidney.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500023)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500023)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500023)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500023)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GSC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500023)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GSC monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSC (RC209586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSC.
HEK293T cells transfected with either RC209586 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GSC antibody(TA500023), and then analyzed by flow cytometry.
Anti-GSC mouse monoclonal antibody (TA500087) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY GSC(RC209586)
Immunofluorescent staining of HeLa cells using anti-GSC mouse monoclonal antibody (TA500087).
WB Suggested Anti-GSC Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: 721_B cell lysate
Host: Rabbit; Target Name: GSC; Sample Tissue: 786-0 Whole Cell lysates; Antibody Dilution: 1.0ug/ml
Immunohistochemistry with Human Tonsil lysate tissue at an antibody concentration of 5.0ug/ml using anti-GSC antibody
Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-GSC antibody
- Quick-Filters:
- Cited (1)
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500087 | Homeobox protein goosecoid / GSC antibody |
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| anti-l Anti-GSC, antibody produced in mouse, Clone 4F11, imm, clone OTI4F11 (formerly 4F11) | |||||||
 |
Mouse | IgG2a | OTI4F11 | Purified | Hu | ICC/IF |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
Published recently!
|
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| TA330186 | Homeobox protein goosecoid / GSC antibody |
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| Rabbit Polyclonal Anti-GSC Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | P, WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
||||||
| TA500023 | Homeobox protein goosecoid / GSC antibody |
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| Goosecoid mouse monoclonal antibody, clone OTI1D7 (formerly 1D7) | |||||||
 |
Mouse | IgG1 | OTI1D7 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
||||||
| AM50087PU-N | Homeobox protein goosecoid / GSC (87-186) antibody |
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 |
Mouse | IgG2a | 2C1 | Purified | Hu | E, P, WB |
50 µg /
€530.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN307365 | Gsc - mouse gene knockout kit via CRISPR |
||
 |
Mouse | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN307365BN | Gsc - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN307365LP | Gsc - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN307365RB | Gsc - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Lysates
| Catalog No. | ||
|---|---|---|
| LY406285 | GSC overexpression lysate |
|
|
0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC209586L2V | Lenti ORF particles, GSC (mGFP-tagged) - Human goosecoid homeobox (GSC), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€880.00
|
|
| OriGene Technologies, Inc. | ||
| RC209586L1V | Lenti ORF particles, GSC (Myc-DDK tagged) - Human goosecoid homeobox (GSC) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€780.00
|
|
| OriGene Technologies, Inc. | ||
