Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine)
Western blot / Immunoblot (WB), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of Host cell factor / HCFC1 antibody
Involved in control of the cell cycle. Also antagonizestransactivation by ZBTB17 and GABP2; represses ZBTB17 activation ofthe p15(INK4b) promoter and inhibits its ability to recruit p300.Coactivator for EGR2 and GABP2. Tethers the chromatin modifyingSet1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3histone deacetylase (HDAC) complexes (involved in the activationand repression of transcription, respectively) together. Componentof a THAP1/THAP3-HCFC1-OGT complex that is required for theregulation of the transcriptional activity of RRM1. As part of theNSL complex it may be involved in acetylation of nucleosomalhistone H4 on several lysine residues. In case of human herpessimplex virus (HSV) infection, HCFC1 forms a multiprotein-DNAcomplex with the viral transactivator protein VP16 and POU2F1thereby enabling the transcription of the viral immediate earlygenes.
1. Zhou P et al. Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and E2F1-responsive gene expression via association with host cell factor-1 (HCF-1). J Biol Chem 288:17532-43 (2013).
Human HepG2; WB Suggested Anti-HCFC1 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate. HCFC1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; HCFC1 antibody - N-terminal region (ARP38600_T100) in Human HepG2 cells using Western Blot
Western Blot: HCFC1 Antibody [NB100-68209] - Whole cell lysate (5, 15 and 50_x000d_mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells._x000d_Antibodies: Affinity purified rabbit anti-HCF1 antibody_x000d_A301-399A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B)_x000d_and used for IP at 3 mcg/mg lysate. HCF1 was also_x000d_immunoprecipitated by rabbit anti-HCF1 antibody A301-400A,_x000d_which recognizes a downstream epitope.
Western Blot and Immunoprecipitation: HCF1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.
Human Stomach; WB Suggested Anti-HCFC1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Stomach; HCFC1 antibody - middle region (ARP38601_P050) in Human Stomach cells using Western Blot
Immunohistochemical analysis of HCFC1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.