Host cell factor / HCFC1 antibody

Principal name

Host cell factor / HCFC1 antibody

Alternative names for Host cell factor / HCFC1 antibody

HCF-1, C1 factor, VP16 accessory protein, VCAF, CFF, HCF1, HFC1

SwissProt ID

P51610 (Human), Q61191 (Mouse)

Gene ID

3054 (HCFC1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of Host cell factor / HCFC1 antibody

Involved in control of the cell cycle. Also antagonizestransactivation by ZBTB17 and GABP2; represses ZBTB17 activation ofthe p15(INK4b) promoter and inhibits its ability to recruit p300.Coactivator for EGR2 and GABP2. Tethers the chromatin modifyingSet1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3histone deacetylase (HDAC) complexes (involved in the activationand repression of transcription, respectively) together. Componentof a THAP1/THAP3-HCFC1-OGT complex that is required for theregulation of the transcriptional activity of RRM1. As part of theNSL complex it may be involved in acetylation of nucleosomalhistone H4 on several lysine residues. In case of human herpessimplex virus (HSV) infection, HCFC1 forms a multiprotein-DNAcomplex with the viral transactivator protein VP16 and POU2F1thereby enabling the transcription of the viral immediate earlygenes.

General readings

1. Zhou P  et al. Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and E2F1-responsive gene expression via association with host cell factor-1 (HCF-1). J Biol Chem 288:17532-43 (2013).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Host cell factor / HCFC1 antibody

WB Suggested Anti-HCFC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Stomach Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Host cell factor / HCFC1 antibody

transformed bacteria; . Lanes: Lane1: HIS-HCFC1 16-363aa (42kD) transformed bacteria lysate. Lane2: GFP-HCFC1 363-2002aa (73kD) transformed bacteria lysate elution sample. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit AlexaFluor 680. Secondary Antibody Dilution: 1:10000. Gene Name: HCFC1. Submitted by: Anonymous.; HCFC1 antibody - middle region (ARP38601_P050) in transformed bacteria cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Host cell factor / HCFC1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HCFC1 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Host cell factor / HCFC1 antibody

Lanes: Lane1: HIS-HCFC1 16-363aa (42kD) transformed bacteria lysate; Lane2: GFP-HCFC1 363-2002aa (73kD) transformed bacteria lysate elution sample; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit AlexaFluor 680; Secondary Antibody Dilu Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Host cell factor / HCFC1 antibody

Host: Rabbit; Target Name: HCFC1; Sample Tissue: Uterus Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Host cell factor / HCFC1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HCFC1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

Host cell factor / HCFC1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HCFC1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

Host cell factor / HCFC1 antibody

transformed bacteria; . Lanes: Lane1: HIS-HCFC1 16-363aa (42kD) transformed bacteria lysate. Lane2: GFP-HCFC1 363-2002aa (73kD) transformed bacteria lysate elution sample. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit AlexaFluor 680. Secondary Antibody Dilution: 1:10000. Gene Name: HCFC1. Submitted by: Anonymous.; HCFC1 antibody - N-terminal region (ARP38600_T100) in transformed bacteria cells using Western Blot Rabbit IgG Purified African clawed frog, Bov, Can, Hu, Ms, Rb, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

Host cell factor / HCFC1 antibody

Immunohistochemical analysis of HCFC1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Host cell factor / HCFC1 antibody

Immunohistochemical analysis of HCFC1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Ze P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Host cell factor / HCFC1 antibody

Immunohistochemical analysis of HCFC1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Ze P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Host cell factor / HCFC1 antibody

Western Blot: HCFC1 Antibody [NB100-68209] - Whole cell lysate (5, 15 and 50_x000d_
mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells._x000d_
Antibodies: Affinity purified rabbit anti-HCF1 antibody_x000d_
A301-399A used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B)_x000d_
and used for IP at 3 mcg/mg lysate. HCF1 was also_x000d_
immunoprecipitated by rabbit anti-HCF1 antibody A301-400A,_x000d_
which recognizes a downstream epitope. Rabbit IgG Aff - Purified Hu IP, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Host cell factor / HCFC1 antibody

Western Blot and  Immunoprecipitation: HCF1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Host cell factor / HCFC1

Host cell factor / HCFC1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Host cell factor / HCFC1

Host cell factor / HCFC1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Host cell factor / HCFC1

Host cell factor / HCFC1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Host cell factor / HCFC1

Host cell factor / HCFC1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

17 Item(s)

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