HPRT1 / HPRT antibody

Principal name

HPRT1 / HPRT antibody

Alternative names for HPRT1 / HPRT antibody

HGPRTase, HGPRT, Hypoxanthine-guanine phosphoribosyltransferase

SwissProt ID

P00492 (Human), P00493 (Mouse), P27605 (Rat), Q04178 (Yeast), Q3SZ18 (Bovin), Q45FY6 (Pig), Q6WIT9 (Canfa), Q9W719 (Chick)

Gene ID

3251 (HPRT1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Ze (Zebrafish), African clawed frog, Xen (Xenopus)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of HPRT1 / HPRT antibody

HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate.HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Reichardt,J.K., (2008) Nippon Rinsho 66 (4), 687-693

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HPRT1 / HPRT antibody

Sample (30 ug of whole cell lysate). A: Molt-4 . B: Raji. 12% SDS PAGE. TA308902 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HPRT1 / HPRT antibody

Sample (30 ug of whole cell lysate). A: IMR32 12% SDS PAGE. TA308901 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HPRT1 / HPRT antibody

Western blot analysis of extracts of various cell lines, using HPRT1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HPRT1 / HPRT antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPRT1 (RC200462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPRT1. Mouse IgG1 OTI1H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HPRT1 / HPRT antibody

Anti-HPRT1 mouse monoclonal antibody (TA506950) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HPRT1(RC200462). Mouse IgG1 OTI1D6 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HPRT1 / HPRT antibody

Anti-HPRT1 mouse monoclonal antibody (TA506952) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HPRT1(RC200462). Mouse IgG1 OTI1B1 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HPRT1 / HPRT antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPRT1 (RC200462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPRT1. Mouse IgG1 OTI2H5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HPRT1 / HPRT antibody

Anti-HPRT1 mouse monoclonal antibody (TA506954) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HPRT1(RC200462). Mouse IgG1 OTI2F7 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HPRT1 / HPRT antibody

Immunofluorescent staining of HeLa cells using anti-HPRT1 mouse monoclonal antibody (TA506987) at 1:100 dilution. Mouse IgG1 OTI2D5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HPRT1 / HPRT antibody

Western blot analysis: The extracts of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT2G8 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

HPRT1 / HPRT antibody

Western blot analysis: The extracts of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT2G8 Purified Hu E, WB
50 µl / €230.00
  Acris Antibodies GmbH

HPRT1 / HPRT (C-term) antibody

Western blot analysis of HPRT1 antibody (C-term) (AP17483PU-N) in Hela cell line lysates (35ug/lane). HPRT1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HPRT1 / HPRT antibody

HPRT1 / HPRT Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

HPRT1 / HPRT antibody

Western Blot analysis of HPRT1 expression in transfected 293T cell line (H00003251-T02) by HPRT1 MaxPab polyclonal antibody.

Lane 1: HPRT1 transfected lysate(24.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Aff - Purified Hu E(detection), WB
50 µg / €380.00
  Abnova Taiwan Corp.

HPRT1 / HPRT antibody

HPRT1 monoclonal antibody (M01), clone 4C3-G8 Western Blot analysis of HPRT1 expression in MCF-7 ( Cat # L046V1 ). Mouse IgG 4C3-G8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HPRT1 / HPRT antibody

Human HeLa; WB Suggested Anti-HPRT1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Hela cell lysate. HPRT1 is supported by BioGPS gene expression data to be expressed in HeLa.; HPRT1 antibody - middle region (ARP48471_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

HPRT1 / HPRT antibody

Western  Blot:  HPRT Antibody [NBP1-33527] – Western blot analysis of HPRT on Molt-4 (Lane A) and Raji (Lane B) lysates using NBP1-33527 at a dilution of 1:1000. 30ug whole cell lysate loaded on 12% SDS PAGE gel. Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

HPRT1 / HPRT antibody

Western Blot: HPRT Antibody (1F8D11) [NBP1-47358] - Western blot analysis using anti-HPTR monoclonal antiobdy against truncated HPRT recombinant protein. Mouse IgG2b 1F8D11 Purified Hu E, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

HPRT1 / HPRT antibody

Western Blot: HPRT Antibody [NBP1-52903] - This Anti-HPRT1 antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rb, Rt, Xen, Ze WB
50 µg / €440.00
  Novus Biologicals Inc.

HPRT1 / HPRT antibody

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

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