HRAS antibody

Principal name

HRAS antibody

Alternative names for HRAS antibody

GTPase HRas, HRAS1, p21ras, H-Ras-1, c-H-ras, Ha-Ras, H-Ras, v-Ha-ras Harvey rat sarcoma viral oncogene homolog

SwissProt ID

P01112 (Human), P08642 (Chick), P20171 (Rat), Q61411 (Mouse)

Gene ID

3265 (HRAS), 6326523 (B2SAT5)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey), Chk (Chicken), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Chicken, Rat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C), Luminex (LMNX)

Background of HRAS antibody

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Prior IA, Harding A, Yan J, Sluimer J, Parton RG, Hancock J.F.: GTP-dependent
segregation of H-ras from lipid rafts is required for biological activity. Nat Cell Biol. 2001
Apr;3(4):368-75.
2. Zheng Y, Quilliam L.A: Activation of the Ras superfamily of small GTPases. Workshop on
exchange factors. EMBO Rep. 2003 May;4(5):463-8.
3. Rotblat B, Prior IA, Muncke C, Parton RG, Kloog Y, Henis YI, Hancock J.F.: Three
separable domains regulate GTP-dependent association of H-ras with the plasma
membrane. Mol Cell Biol. 2004 Aug;24(15):6799-810.
4. Roy S, Plowman S, Rotblat B, Prior IA, Muncke C, Grainger S, Parton RG, Henis YI, Kloog
Y, Hancock J.F.: Individual palmitoyl residues serve distinct roles in H-ras trafficking,
microlocalization, and signaling. Mol Cell Biol. 2005 Aug;25(15):6722-33.
5. Rotblat B, Yizhar O, Haklai R, Ashery U, Kloog Y.: Ras and its signals diffuse through the
cell on randomly moving nanoparticles. Cancer Res. 2006 Feb 15;66(4):1974-81.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HRAS antibody

H-Ras antibody detects HRAS protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. H-Ras antibody (TA308498) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HRAS antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505666) Mouse IgG1 OTIC8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HRAS antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505667) Mouse IgG2b OTIC2 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HRAS antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505669) Mouse IgG1 OTID9 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

HRAS antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505675) Mouse IgG1 OTIA1 Purified Can, Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HRAS antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505676) Mouse IgG1 OTI1A1 Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HRAS antibody

Western blot analysis of extracts from HeLa cells, using RASH/RASK/RASN antibody (#TA326254).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HRAS antibody

Anti-HRAS mouse monoclonal antibody (TA505665) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HRAS(RC216409). Mouse IgG1 OTI1F2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HRAS antibody

Anti-HRAS mouse monoclonal antibody (TA505668) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HRAS(RC216409). Mouse IgG1 OTIG8 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HRAS antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS. Mouse IgG1 OTI1H3 Purified Can, Hu, Mky, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HRAS antibody

HRAS Luminex Elisa with 1G8 Capture (TA600224) and 1A1 Detection (TA700224) Antibodies. Substrate used: Recombinant Human HRAS (TP316409) Mouse IgG1 OTI1G8 Purified Hu LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

HRAS antibody

HRAS Luminex Elisa with 1G8 Capture (TA600224) and 1A1 Detection (TA700224) Antibodies. Substrate used: Recombinant Human HRAS (TP316409) Mouse IgG1 OTI1A1 Purified Hu LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

HRAS antibody

Western Blotting analysis (reducing conditions) of H-Ras in whole cell lysate using anti-H-Ras (H-RAS-03). Lane 1: HeLa human cervix carcinoma cell line. Lane 2: K567 human leukemia cell line. Lane 3: RAJI human Burkitt lymphoma cell line. Mouse IgG1 H-Ras-03 Aff - Purified Hu E, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

HRAS (Center) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with HRAS antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HRAS (C-term) antibody

Confocal immunofluorescent analysis of HRAS Antibody (C-term) Cat.-No AP52096PU-N with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). Rabbit Ig Purified Hu, Ms ICC/IF, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HRAS antibody

HRAS Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

HRAS antibody

Western Blot analysis of HRAS expression in transfected 293T cell line (H00003265-T02) by HRAS MaxPab polyclonal antibody.

Lane 1: HRAS transfected lysate(20.79 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

HRAS antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and HRAS HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-HRAS mouse purified polyclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

HRAS antibody

Western Blot analysis of HRAS expression in transfected 293T cell line (H00003265-T01) by HRAS MaxPab polyclonal antibody.

Lane 1: HRAS transfected lysate(21.30 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

HRAS antibody

Western Blotting analysis ( reducing conditions ) of HRAS in whole cell lysate using HRAS monoclonal antibody, clone H-RAS-03 ( Cat # MAB3617 ).
Lane 1 : HeLa, human cervix carcinoma cell line.
Lane 2 : K-567, human leukemia cell line.
Lane 3 : RAJI, human Burkitt lymphoma cell line. Mouse IgG1 H-RAS-03 Hu WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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