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HSBP1 / HSF1BP antibody

Principal name

HSBP1 / HSF1BP antibody

Alternative names for HSBP1 / HSF1BP antibody

Heat shock factor-binding protein 1, NPC-A-13, Nasopharyngeal carcinoma-associated antigen 13

SwissProt ID

O75506 (Human), Q3ZC22 (Bovin), Q8K3X8 (Rat), Q9CQZ1 (Mouse)

Gene ID

3281 (HSBP1)

Ncbi ID

NP_001528.1, NP_001528, 9606, NM_001537

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of HSBP1 / HSF1BP antibody

The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response.The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response.

General readings

Tai,L.J., (2002) J. Biol. Chem. 277 (1), 735-745

Immunohistochemical of paraffin-embedded human prostate cancer using 10169-2-AP(HSBP1 antibody) at dilution of 1:50 (under 10x lens)

10169-2-AP

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP(HSBP1 antibody) at dilution of 1:1000

10169-2-AP

HT-1080 cells were subjected to SDS PAGE followed by western blot with 10169-2-AP(HSBP1 antibody) at dilution of 1:300

10169-2-AP

Human brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP(HSBP1 antibody) at dilution of 1:500

10169-2-AP

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP(HSBP1 antibody) at dilution of 1:300

10169-2-AP

Human Brain; WB Suggested Anti-HSBP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; HSBP1 antibody - N-terminal region (ARP35643_P050) in Human Brain cells using Western Blot

ARP35643_P050

SDS-Page: HSBP1 Protein [NBC1-18355] - HSBP1, 8.5kDa (76aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

NBC1-18355

Immunohistochemistry-Paraffin: HSBP1 Antibody [NBP1-86373] Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity with a granular pattern in glandular cells.

NBP1-86373

HS6ST1 monoclonal antibody (M05A), clone 1H4. Western Blot analysis of HS6ST1 expression in IMR-32(Cat # L008V1 ).

Western blot experiment using HSBP1 Antibody (GTX110661) at 1:90000 dilution to detect 100ng of 13kDa recombinant HSBP1 protein antigen.

GTX110661

TP301399

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA339061	HSBP1 / HSF1BP antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze	WB	50 µg / €325.00
							OriGene Technologies, Inc.
H00003281-M02	HSBP1 / HSF1BP antibody
	Mouse	IgG2a	2C3	Purified	Hu	E, WB	0.1 mg / €370.00
							Abnova Taiwan Corp.
ARP35643_P050	HSBP1 / HSF1BP antibody
	Rabbit			Aff - Purified	African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze	WB	50 µg / €350.00
							AVIVA Systems Biology
NBP1-86373	HSBP1 / HSF1BP antibody
	Rabbit	IgG		Aff - Purified	Hu	P	0.1 ml / €500.00
							Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
TP301399	HSBP1 / HSF1BP
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00
					OriGene Technologies, Inc.

SA6022	HSBP1 / HSF1BP
	Human	Purified	> 95 % by SDS-PAGE	E. coli	0.1 mg / €230.00
					Acris Antibodies GmbH
+1 additional image
SA6022X	HSBP1 / HSF1BP
	Human	Purified	> 95 % by SDS-PAGE	E. coli	0.5 mg / €540.00
					Acris Antibodies GmbH
+1 additional image
H00003281-P01-10	HSBP1 / HSF1BP
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.	10 µg / €380.00
					Abnova Taiwan Corp.
H00003281-P01-25	HSBP1 / HSF1BP
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.	25 µg / €510.00
					Abnova Taiwan Corp.
NBC1-18355	HSBP1 / HSF1BP
			Protein		0.1 mg / €310.00
					Novus Biologicals Inc.

Lysates (Overexpressed)

Catalog No.
LY419869	HSBP1 overexpression lysate
		0.1 mg / €280.00
		OriGene Technologies, Inc.

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