HSP10 antibody

Principal name

HSP10 antibody

Alternative names for HSP10 antibody

HSPE1, 10 kDa heat shock protein,10 kDa chaperonin, CPN10, Early-pregnancy factor

SwissProt ID

P61603 (Bovin), P61604 (Human), Q64433 (Mouse)

Gene ID

3336 (HSPE1), 15528 (Hspe1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Sh (Sheep), GP (Guinea Pig), Rb (Rabbit), Xen (Xenopus), Eq (Equine), Gt (Goat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of HSP10 antibody

HSPE1(heat shock 10kDa protein 1 (chaperonin 10)), also called CPN10, GROES, CHAPERONIN 10 HOMOLOG, cpn10 HOMOLOG or HSP10, is a protein that in humans is encoded by the HSPE1 gene. GroES is a heptameric ring of identical 10.4-kD subunits that binds to each end of GroEL to form a symmetric, functional heterodimer. The HSP10 gene consists of 4 exons. The HSPE1 gene is mapped to 2q33.1. The transcriptional activity of the promoter fragment in the HSP60 direction is approximately twice that in the HSP10 direction under normal growth conditions; upon heat shock, promoter activity in either direction increased by a factor of approximately 12. Mutational drifts performed in vitro with 4 different enzymes indicated the GroES overexpression doubled the number of accumulating mutations, and promoted the folding of enzyme variants carrying mutations in the protein core and/or mutations with higher destabilizing effects.

General readings

1. Georgopoulos, C., Welch, W. J. Role of the major heat shock proteins as molecular
chaperones. Annu. Rev. Cell Biol. 9: 601-634, 1993.
2. Hansen, J. J., Bross, P., Westergaard, M., Nielsen, M. N., Eiberg, H., Borglum, A. D., Mogensen, J.,
Kristiansen, K., Bolund, L., Gregersen, N. Genomic structure of the human mitochondrial chaperonin
genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a
bidirectional promoter. Hum. Genet. 112: 71-77, 2003. Note: Erratum: Hum. Genet. 112: 436 only,
2003.
3. Todd, M. J., Viitanen, P. V., Lorimer, G. H. Dynamics of the chaperonin ATPase cycle: implications for
facilitated protein folding. Science 265: 659-666, 1994.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HSP10 antibody

Western blot analysis of extracts from NIH/3T3 cells, using HSP10 antibody (#TA313120).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSP10 antibody

Immunofluorescence of monoclonal antibody to HSPE1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 4C11-B11 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HSP10 antibody

Western blot analysis of Cpn10 in rat brain extract using a 1:1000 dilution of the antibody Rabbit Purified Bov, Can, GP, Hu, Ms, Por, Rb, Rt, Sh, Xen WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSP10 antibody

WB Suggested Anti-HSPE1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human brain Rabbit IgG Purified Can, Eq, GP, Gt, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

HSP10 antibody

Western blot analysis on NIH-3T3 cell lysate using HSP10 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSP10 antibody

Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: HepG2 cells, Primary antibody: (HSPE1 Antibody) at dilution 1/750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSP10 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HSP10 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

HSP10 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HSP10 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

HSP10 antibody

Human Brain; WB Suggested Anti-HSPE1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; HSPE1 antibody - middle region (ARP54651_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

HSP10 antibody

HSP10 Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €290.00
  Boster Immunoleader

HSP10 antibody

Immunohistochemical analysis of HSPE1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Sh ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

HSP10 antibody

Immunohistochemical analysis of HSPE1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Sh ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

HSP10 antibody

Immunohistochemical analysis of HSPE1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Sh ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

HSPE1 - human gene knockout kit via CRISPR

HSPE1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

HSPE1 - human gene knockout kit via CRISPR

HSPE1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hspe1 - mouse gene knockout kit via CRISPR

Hspe1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Hspe1 - mouse gene knockout kit via CRISPR

Hspe1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

HSP10

HSP10 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

HSP10 (1-102) (recombinant)

HSP10 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

HSP10 (1-102) (recombinant)

HSP10 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

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