HSP60 antibody

Principal name

HSP60 antibody

Alternative names for HSP60 antibody

HSP-60, HSPD1, Heat shock protein 60, Chaperonin 60, HuCHA60, GROEL, GroEL Homolog, CPN60, 60 kDa heat shock protein mitochondrial, 60 kDa chaperonin, Mitochondrial matrix protein P1, P60 lymphocyte protein

SwissProt ID

P0A6F5 (Ecoli), P10809 (Human), P31081 (Bovin), P63038 (Mouse), P63039 (Rat), Q3KMQ9 (Chlta)

Gene ID

948665 (groL), 3329 (HSPD1), 63868 (Hspd1)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Prim (Primate), Bac (Bacteria), Bov (Bovine), Can (Canine), Chk (Chicken), GP (Guinea Pig), Hst (Hamster), Pl (Plant), Por (Porcine), Rb (Rabbit), Ye (Yeast), Chimp (Chimpanzee), E. coli (Escherichia coli), Dros (Drosophila), Fi (Fish), Re (Reptiles), All Species, Sh (Sheep), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Radioimmunoassay (R), EPair for Elisa (EPair)

Background of HSP60 antibody

Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65 kDa major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrial matrix and in plants Hsp60 is localized in the chloroplast. Mitochondria, chloroplasts and bacteria have a common ancestry (>1 billion years) and this fact together with the high degree of homology between the divegent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of magnesium ions and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single ring human mitochondrial homolog, Hsp60 with its co chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co chaperonin, GroES. Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing. Data demonstrates that recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase 3 by cytochrome c and dATP in an ATP dependent manner. Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, however several recent studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, artherosclerosis and chlamydial disease.

General readings

General / background references:Rook G & McCulloch J HLA-DR4, mycobacteria, heat-shock proteins, and rheumatoid arthritis. Arthritis Rheum 35:1409-12 (1992). PubMed PMID: 1472119
Sharif M et al. The development of monoclonal antibodies to the human mitochondrial 60-kd heat-shock protein, and their use in studying the expression of the protein in rheumatoid arthritis. Arthritis Rheum 35:1427-33 (1992). PubMed PMID: 1361734
Langer T et al. Chaperonin-mediated protein folding: GroES binds to one end of the GroEL cylinder, which accommodates the protein substrate within its central cavity. EMBO J 11:4757-65 (1992). PubMed PMID: 1361169

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HSP60 antibody

Western blot analyis of Hsp60 in HeLa cell lysates using a 1:1000 dilution of the antibody Mouse IgG1 LK2 Purified Bac, Bov, Can, Chk, GP, Hst, Hu, Mky, Ms, Pl, Por, Rb, Rt, whitefly, Ye C, F, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

HSP60 antibody

Western blot analyis of Hsp60 in HeLa cell lysates using a 1:1000 dilution of the antibody Mouse IgG1 LK2 Purified Bac, Bov, Can, Chk, GP, Hst, Hu, Mky, Ms, Pl, Por, Rb, Rt, whitefly, Ye C, F, WB
50 µg / €210.00
  Acris Antibodies GmbH

HSP60 antibody

Western blot analysis of extracts from COLO205 cells, using HSP60 antibody (#TA311958).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSP60 antibody

Western Blot: Hsp60 Antibody [TA336825] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7 Rabbit Purified Hst, Hu, Ms, Prim, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSP60 antibody

Western Blot: Hsp60 Antibody [TA336826] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7 Rabbit Purified Hst, Hu, Ms, Prim, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSP60 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800756) Mouse IgG1 OTIE4 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €248.00
  OriGene Technologies, Inc.

TrueMAB

HSP60 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758) Mouse IgG2a OTIA2 Purified Can, Hu, Mky, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HSP60 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771) Mouse IgG2a OTIG1 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HSP60 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772) Mouse IgG1 OTIF7 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

HSP60 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774) Mouse IgG1 OTIF11 Purified Can, Hu, Mky, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HSP60 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847) Mouse IgG1 OTI3G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HSP60 (1-573) antibody

Immunohistochemistry:Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60Cat.No. AM03128PU (1/50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Mouse IgG1 2E4 Purified Hu E, F, ICC/IF, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

HSP60 (1-573) antibody

Immunohistochemistry:Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60Cat.No. AM03128PU (1/50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Mouse IgG1 2E4 Purified Hu E, F, ICC/IF, P, WB
50 µl / €220.00
  Acris Antibodies GmbH

HSP60 antibody

Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and colon cancer tissues (right) using HSP60 antibody Cat.-No AM06606SU-N with DAB staining. Mouse IgG1 3G8 Ascites Hu, Mky, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

HSP60 antibody

Human Uterus (formalin-fixed, paraffin-embedded) stained with HSPD1 antibody AP22882PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Purified Hu IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

HSP60 antibody

Immunofluorescence of purified MaxPab antibody to HSPD1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

HSP60 antibody

Immunofluorescence of purified MaxPab antibody to HSPD1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

HSP60 antibody

HSP60 Mouse IgG1 GROEL730 Purified Hu C, F, ICC/IF, IP, P, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

HSP60 antibody

HSP60 Mouse IgG1 GROEL730 Purified Hu C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

HSP60 antibody

HSP60 Mouse IgG1 GROEL730 Purified Hu C, F, ICC/IF, IP, P, WB
20 µg / €160.00
  Acris Antibodies GmbH

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