HSP90AA1 / HSP90 alpha antibody

Principal name

HSP90AA1 / HSP90 alpha antibody

Alternative names for HSP90AA1 / HSP90 alpha antibody

HSP90A, HSPC1, HSPCA, Heat shock 86 kDa, HSP86, NY-REN-38, Heat shock protein HSP 90-alpha

SwissProt ID

O02705 (Pig), P07900 (Human), P07900-2, P07901 (Mouse), P11501 (Chick), P30946 (Rabit), P46633 (Crigr), P82995 (Rat), Q76LV2 (Bovin), Q9GKX7 (Horse)

Gene ID

3320 (HSP90AA1), 423463, 15519 (Hsp90aa1), 299331

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Rb (Rabbit), Can (Canine), Chk (Chicken), Por (Porcine), Av (Birds), Mky (Monkey), Fi (Fish), S. cerevisiae (Saccharomyces cerevisiae), Dros (Drosophila), Frog, Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Radioimmunoassay (R), Flow Cytometry (F)

Background of HSP90AA1 / HSP90 alpha antibody

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer.(2) From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, Hsp90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively.
Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (9).

General readings

Pritchard, K. A., et al., (2001) J Biol Chem 276:17621-17624.
Miyamoto, A., et al., (2002) Nature 416:865-869.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Heat Shock Protein 90 (HSP90) alpha/beta antibody

  Mouse Monoclonal anti-HSP90AA1 Antibody  
Western blot analysis of Hsp90 in cell lysates from 12 rat tissue lines at a 1:1000 dilution of TA309402. Mouse IgG2a Hyb-K41220A Purified Hu, Rt, S. cerevisiae, S.pombe C, E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat Shock Protein 90 (HSP90) alpha/beta antibody

Western blotting analysis of Hsp90 alpha and beta protein by antibody MBH90AB to both Hsp90 alpha and beta isoform. Mouse IgG1 MBH90AB Aff - Purified Bov, Hu, Ms IP, WB
0.1 mg / €290.00
  Acris Antibodies GmbH

Heat Shock Protein 90 Complex (HSP90) antibody

  Mouse Monoclonal anti-HSP90AB1 Antibody  
Hsp90 complex isolation (IP) from rabbit reticulocyte lysate (8D3), SDS PAGE. Mouse IgM 8D3 Purified Hu, Ms, Rb, Rt IP
50 µg / €325.00
  OriGene Technologies, Inc.

Heat Shock Protein 90 Complex (HSP90) antibody

  Mouse monoclonal Hsp90 complex Antibody  
Hsp90 complex isolation (IP) from rabbit reticulocyte lysate (8D3), SDS PAGE. Mouse IgM 8D3 Purified Hu, Ms, Rb, Rt IP
0.2 mg / €325.00
  OriGene Technologies, Inc.

Heat Shock Protein 90 / HSP90 (alpha + beta) antibody

Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG2b 4F10 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Heat Shock Protein 90 / HSP90 (alpha + beta) antibody

Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG2b 4F10 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Heat Shock Protein 90 / HSP90 (C-term) antibody

Heat Shock Protein 90 / HSP90 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

HSP90 antibody

Human Spleen (formalin-fixed, paraffin-embedded) stained with Heat Shock Protein 90 (HSP90) antibody AP22747PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms, Rb, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

HSP90AA1 / HSP90 alpha antibody

  Mouse monoclonal Hsp90 alpha Antibody  
Western blot analysis of Hsp90Alpha in rat tissues, using a 1:1000 dilution of the antibody Mouse IgG1 2G5.G3 Aff - Purified Hu, Ms, Rt C, E, ICC/IF, IP, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

HSP90AA1 / HSP90 alpha antibody

  anti-HSP90AA1 (Hsp90 alpha) mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)  
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-HSP90AA1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500883, Dilution 1:50) Mouse IgG1 OTI3B5 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

HSP90AA1 / HSP90 alpha antibody

Immunohistochemical analysis of paraffin-embedded lung cancer (left) and  bladder cancer (right) tissues using HSP90AA1 antibody Cat.-No AM06760PU-N with DAB staining. Mouse IgG1 5G5 Purified Hu, Mky, Ms E, F, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

HSP90AA1 / HSP90 alpha (Acetyl K294) antibody

  Rabbit polyclonal HSP 90 K294 antibody  
WB using Anti-Hsp90 acetyl K294 antibody shows detection of a band at ~90 kDa corresponding to Hsp90 in an SkBr3 cell lysate (arrowhead) after treatment with Trichostatin A (an HDAC inhibitor). WBting results do not definitively demonstrate the acetyl K294 specificity of this reagent because similar staining is seen in the control lane (no treatment with TSA). IP with anti-Hsp90 was performed prior to WBting with anti-Hsp90 acetyl K294. Rabbit IgG Aff - Purified Chk, Dros, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

HSP90AA1 / HSP90 alpha (289-300) antibody

  Rabbit polyclonal Hsp 90 alpha antibody  
WB using Anti-Hsp90 antibody shows detection of a band at ~90 kDa corresponding to Hsp90 in various lysate preparations (arrowhead). Cos7 transfected with mutant K294R Hsp90 (left two lanes), Cos7 transfected with wild type Hsp90 (middle two lanes) and empty vector Cos7 cells (right two lanes) were either treated with Trichostatin A (an HDAC inhibitor) or mock treated with DMSO only. Anti-FlagTM immunoprecipitation was performed prior to WB with anti-Hsp90. Rabbit IgG Aff - Purified Chk, Dros, Hu, Mky, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

HSP90AA1 / HSP90 alpha antibody

  Mouse monoclonal Hsp90 alpha Antibody  
Western blot analysis of Hsp90alpha in rat tissues using a 1:1000 dilution of the antibody Mouse IgG2a Hyb-K41009 Aff - Purified Hu, Rt C, E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSP90AA1 / HSP90 alpha antibody

  Mouse Monoclonal Anti-Hsp90 Antibody  
Western blot analysis of Hsp90 in rat tissue lysates using a 1:1000 dilution of the antibody Mouse IgG1 D7alpha Purified Bov, Chk, Hu, Ms, Por, Rb, Rt C, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSP90AA1 / HSP90 alpha antibody

  Rabbit polyclonal anti-HSP90AA1(HSP90) antibody, Loading control  
Western blot analysis of extracts of various cell lines, using HSP90AA1 antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSP90AA1 / HSP90 alpha antibody

  Rabbit Polyclonal HSP90 alpha Antibody  
Immunohistochemistry: Hsp90A Antibody [TA336841] - Analysis of Hsp901a in human kidney carcinoma using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSP90AA1 / HSP90 alpha antibody

  Rabbit Polyclonal HSP90 alpha Antibody  
Western Blot: Hsp90A Antibody [TA336843] - Analysis of HSP901a in: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. PC12, 8. COS7 Rabbit Purified Hu, Ms, Prim, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSP90AA1 / HSP90 alpha antibody

  Rabbit Polyclonal Anti-HSP90A Antibody  
Western blot analysis on NIH-3T3 cell lysate using HSP90A Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSP90AA1 / HSP90 alpha antibody

  Rabbit Polyclonal Anti-HSP90AA1 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: A549 cells, 293T cells, mouse brain tissue, K562 cells, Hela cells, Primary antibody: (HSP90AA1 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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