HSPA4 / APG2 antibody
Principal name
HSPA4 / APG2 antibody
Alternative names for HSPA4 / APG2 antibody
HSP70RY, HSP70-4, Heat shock 70-related protein APG-2
Gene ID
3308 (HSPA4)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of HSPA4 / APG2 antibody
HSPA4(Hsp70) belongs to the heat shock protein 70 family. It was isolated as a putative Rictor interacting protein and interaction with membranes acts as a platform for its release into the extracellular environment during its recovery from stress. Hsp70 gene expression in Rheumatoid Arthitis-affected synovial tissue is followed by Hsp70 cell surface expression on fibroblast-like synovial cells growing from RA synovial tissue. Serum Hsp70 levels were increased in Systemic sclerosis patients, and associated with pulmonary fibrosis, skin sclerosis, renal vascular damage, oxidative stress, and inflammation.
General readings
Kim,H.E., (2008) Mol. Cell 30 (2), 239-247
Human 293T; WB Suggested Anti-HSPA4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate; HSPA4 antibody - N-terminal region (ARP46073_P050) in Human 293T cells using Western Blot
Human 721_B; Host:Rabbit. Target Name:HSPA4. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. HSPA4 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; HSPA4 antibody - middle region (ARP46074_P050) in Human 721_B cells using Western Blot
Human Brain; WB Suggested Anti-HSPA4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; HSPA4 antibody - middle region (ARP46074_P050) in Human Brain cells using Western Blot
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using HSP70 4(GTX112329) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 7.5% SDS PAGE GTX112329 diluted at 1:1000
HSPA4 MaxPab polyclonal antibody. Western Blot analysis of HSPA4 expression in human liver.
Western Blot analysis of HSPA4 expression in transfected 293T cell line (H00003308-T02) by HSPA4 MaxPab polyclonal antibody.Lane 1: HSPA4 transfected lysate(92.4 KDa).Lane 2: Non-transfected lysate.
HSPA4 MaxPab polyclonal antibody. Western Blot analysis of HSPA4 expression in HeLa.
HSPA4 MaxPab rabbit polyclonal antibody. Western Blot analysis of HSPA4 expression in mouse spleen.
Western Blot analysis of HSPA4 expression in transfected 293T cell line (H00003308-T04) by HSPA4 MaxPab polyclonal antibody.Lane 1: HSPA4 transfected lysate(94.30 KDa).Lane 2: Non-transfected lysate.
HSPA4 MaxPab rabbit polyclonal antibody. Western Blot analysis of HSPA4 expression in Jurkat.
Western Blot analysis of HSPA4 expression in transfected 293T cell line by HSPA4 monoclonal antibody (M01), clone 3A11.Lane 1: HSPA4 transfected lysate(94.3 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed, paraffin embedded human breast carcinoma stained with HSPA4 monoclonal antibody, clone W27 ( Cat # MAB3737 ).
Immunoprecipitation : Use at 2 µg/mg protein lysate. Use Protein A.
Western Blot: HSPA4 Antibody [NBP1-33425] – Western blot analysis of HSPA4 on Hep G2 lysates using NBP1-33425 at a dilution of 1:1000. 30ug whole cell lysate loaded on 7.5% SDS PAGE gel.
Western Blot: HSPA4 Antibody [NBP1-54348] - This Anti-HSPA4 antibody was used in Western Blot of Fetal Brain tissue lysate at a concentration of 1ug/ml.
Immunohistochemistry-Paraffin: HspA4 Antibody [NBP1-81696] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
Immunofluorescence: HspA4 Antibody [NBP1-81696] - 431 shows positivity in nucleus but not nucleoli & cytoplasm.
Western Blot: HspA4 Antibody [NBP1-81696] Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Immunohistochemical staining of HSPA4 on formalin fixed, paraffin embedded human breast cancer with HSPA4 polyclonal antibody.
Immunohistochemical analysis of paraffin-embedded human breast cancer using HSP70 mouse mAb with DAB staining.
Immunofluorescence analysis of NIH/3T3 cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Western blot analysis using HSP70 mouse mAb against Hela (1) cell lysate.
Immunohistochemical analysis of paraffin-embedded Hepatoma, using HSPA4(GTX112607) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate) A: Raji B: K562 5% SDS PAGE GTX112607 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NIH-3T3 5% SDS PAGE GTX112607 diluted at 1:500
WB Suggested Anti-HSPA4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA339251 | HSPA4 / APG2 antibody |
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 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze | WB |
50 µg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA339252 | HSPA4 / APG2 antibody |
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 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM06455SU-N | HSPA4 / APG2 antibody |
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 |
Mouse | IgG1 | 5A6 | Ascites | Hu | E, ICC/IF, P, WB |
0.1 ml /
€400.00
|
| Acris Antibodies GmbH | |||||||
 +2 additional images |
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| H00003308-B02P | HSPA4 / APG2 antibody |
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 |
Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
|
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| Abnova Taiwan Corp. | |||||||
| +2 additional images |
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| H00003308-D01 | HSPA4 / APG2 antibody |
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|
Rabbit | Serum | Hu, Ms | E(detection), IP, WB |
0.1 ml /
€380.00
|
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| Abnova Taiwan Corp. | |||||||
| H00003308-D01P | HSPA4 / APG2 antibody |
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 |
Rabbit | Purified | Hu, Ms | E(detection), WB |
0.1 mg /
€380.00
|
||
| Abnova Taiwan Corp. | |||||||
| +2 additional images |
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| H00003308-M01 | HSPA4 / APG2 antibody |
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 |
Mouse | IgG2a | 3A11 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| MAB3737 | HSPA4 / APG2 antibody |
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 |
Mouse | IgG2a | W27 | Bov, Hu, Mky | F, ICC/IF, IP, P |
0.1 mg /
€820.00
|
|
| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| PAB11679 | HSPA4 / APG2 antibody |
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 |
Rabbit | Hu | P, WB |
0.1 ml /
€390.00
|
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| Abnova Taiwan Corp. | |||||||
| ARP46073_P050 | HSPA4 / APG2 antibody |
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 |
Rabbit | Aff - Purified | African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze | WB |
50 µg /
€350.00
|
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| AVIVA Systems Biology | |||||||
| ARP46074_P050 | HSPA4 / APG2 antibody |
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 |
Rabbit | Aff - Purified | African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| +1 additional image |
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| F-MK40250310 | HSPA4 / APG2 antibody |
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Rabbit | IgG | Aff - Purified | Hu, Ms | IP, WB |
0.1 mg /
€680.00
|
|
| Funakoshi Co., Ltd. | |||||||
| NBP1-33425 | HSPA4 / APG2 antibody |
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![Western Blot: HSPA4 Antibody [NBP1-33425] – Western blot analysis of HSPA4 on Hep G2 lysates using NBP1-33425 at a dilution of 1:1000. 30ug whole cell lysate loaded on 7.5% SDS PAGE gel.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/111765_1.jpg) |
Rabbit | IgG | Hu | P, WB |
0.1 mg /
€420.00
|
||
| Novus Biologicals Inc. | |||||||
| NBP1-54348 | HSPA4 / APG2 antibody |
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![Western Blot: HSPA4 Antibody [NBP1-54348] - This Anti-HSPA4 antibody was used in Western Blot of Fetal Brain tissue lysate at a concentration of 1ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/HSPA4 Antibody-Western Blot-NBP1-54348-img0001.jpg) |
Rabbit | Aff - Purified | Can, Hu, Ms, Rt | WB |
50 µg /
€440.00
|
||
| Novus Biologicals Inc. | |||||||
| NBP1-81696 | HSPA4 / APG2 antibody |
||||||
![Immunohistochemistry-Paraffin: HspA4 Antibody [NBP1-81696] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/HspA4 Antibody-Immunohistochemistry-Paraffin-NBP1-81696-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP320635 | HSPA4 / APG2 |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| H00003308-P01-10 | HSPA4 / APG2 |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00003308-P01-25 | HSPA4 / APG2 |
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Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
Antibody Pairs
| Catalog No. | ||
|---|---|---|
| H00003308-PW1 | HSPA4 (Human) IP-WB Antibody Pair |
|
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1 set /
€710.00
|
|
| Abnova Taiwan Corp. |
Lysates
| Catalog No. | ||
|---|---|---|
| H00003308-T02 | HSPA4 293T Cell Transient Overexpression Lysate(Denatured) |
|
|
0.1 ml /
€270.00
|
|
| Abnova Taiwan Corp. |
