HSPA4 / APG2 antibody
Principal name
HSPA4 / APG2 antibody
Alternative names for HSPA4 / APG2 antibody
HSP70RY, HSP70-4, Heat shock 70-related protein APG-2
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)
Background of HSPA4 / APG2 antibody
HSPA4(Hsp70) belongs to the heat shock protein 70 family. It was isolated as a putative Rictor interacting protein and interaction with membranes acts as a platform for its release into the extracellular environment during its recovery from stress. Hsp70 gene expression in Rheumatoid Arthitis-affected synovial tissue is followed by Hsp70 cell surface expression on fibroblast-like synovial cells growing from RA synovial tissue. Serum Hsp70 levels were increased in Systemic sclerosis patients, and associated with pulmonary fibrosis, skin sclerosis, renal vascular damage, oxidative stress, and inflammation.
General readings
Kim,H.E., (2008) Mol. Cell 30 (2), 239-247
Western Blot analysis of HSPA4 expression in transfected 293T cell line by HSPA4 monoclonal antibody (M01), clone 3A11.Lane 1: HSPA4 transfected lysate(94.3 KDa).Lane 2: Non-transfected lysate.
HSPA4 MaxPab polyclonal antibody. Western Blot analysis of HSPA4 expression in human liver.
Western Blot analysis of HSPA4 expression in transfected 293T cell line (H00003308-T02) by HSPA4 MaxPab polyclonal antibody.Lane 1: HSPA4 transfected lysate(92.4 KDa).Lane 2: Non-transfected lysate.
HSPA4 MaxPab polyclonal antibody. Western Blot analysis of HSPA4 expression in HeLa.
HSPA4 MaxPab rabbit polyclonal antibody. Western Blot analysis of HSPA4 expression in mouse spleen.
Western Blot analysis of HSPA4 expression in transfected 293T cell line (H00003308-T04) by HSPA4 MaxPab polyclonal antibody.Lane 1: HSPA4 transfected lysate(94.30 KDa).Lane 2: Non-transfected lysate.
HSPA4 MaxPab rabbit polyclonal antibody. Western Blot analysis of HSPA4 expression in Jurkat.
Immunohistochemical staining of HSPA4 on formalin fixed, paraffin embedded human breast cancer with HSPA4 polyclonal antibody.
Formalin-fixed, paraffin embedded human breast carcinoma stained with HSPA4 monoclonal antibody, clone W27 ( Cat # MAB3737 ).
Immunoprecipitation : Use at 2 µg/mg protein lysate. Use Protein A.
Human 293T; WB Suggested Anti-HSPA4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate; HSPA4 antibody - N-terminal region (ARP46073_P050) in Human 293T cells using Western Blot
Human 721_B; Host:Rabbit. Target Name:HSPA4. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. HSPA4 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; HSPA4 antibody - middle region (ARP46074_P050) in Human 721_B cells using Western Blot
Human Brain; WB Suggested Anti-HSPA4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; HSPA4 antibody - middle region (ARP46074_P050) in Human Brain cells using Western Blot
Immunohistochemical analysis of paraffin-embedded human breast cancer using HSP70 mouse mAb with DAB staining.
Immunofluorescence analysis of NIH/3T3 cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Western blot analysis using HSP70 mouse mAb against Hela (1) cell lysate.
WB Suggested Anti-HSPA4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA339251 | HSPA4 / APG2 antibody |
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| Rabbit Polyclonal Anti-HSPA4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA339252 | HSPA4 / APG2 antibody |
||||||
| Rabbit Polyclonal Anti-HSPA4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM06455SU-N | HSPA4 / APG2 antibody |
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 |
Mouse | IgG1 | 5A6 | Ascites | Hu | E, ICC/IF, P, WB |
0.1 ml /
€400.00
|
| Acris Antibodies GmbH | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP320635 | HSPA4 / APG2 |
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| Recombinant protein of human heat shock 70kDa protein 4 (HSPA4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY400782 | HSPA4 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR213069L2V | Lenti ORF particles, Hspa4 (GFP-tagged ORF) - Rat heat shock protein 4 (Hspa4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,618.00
|
|
| OriGene Technologies, Inc. | ||
| RR213069L1V | Lenti ORF particles, Hspa4 (Myc-DDK-tagged ORF) - Rat heat shock protein 4 (Hspa4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,452.00
|
|
| OriGene Technologies, Inc. | ||
