HSPA5 / GRP78 antibody

Principal name

HSPA5 / GRP78 antibody

Alternative names for HSPA5 / GRP78 antibody

78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5, BiP, Immunoglobulin heavy chain-binding protein, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78

SwissProt ID

P06761 (Rat), P11021 (Human), P16392 (Horse), P20029 (Mouse), P34935 (Pig), Q0VCX2 (Bovin), Q90593 (Chick), Q91883 (Xenla)

Gene ID

3309 (HSPA5), 14828 (Hspa5), 25617

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Sh (Sheep), Ce (C. elegans), Dros (Drosophila), Mky (Monkey), Ze (Zebrafish), Hst (Hamster), Can (Canine), Rb (Rabbit), Xen (Xenopus), Prim (Primate)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Functional assay (FN), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of HSPA5 / GRP78 antibody

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.The HSP70 proteins are ubiquitous molecular chaparones that are found in all organisms and tissue types. Like other members of the HSP70 family, BiP is a peptide-binding ATPase that is able to differentiate native proteins from unfolded polypeptides. BiP does not bind to fully folded and assembled proteins, except in the presence of other co-chaparones. BiP is involved in a number of key mechanisms and pathways including polypeptide translocation across the endoplasmic reticulum, folding, assembly, transport of secreted or membrane proteins, and the regulation of calcium homeostasis. Although BiP is relatively abundant, marked increases in BiP occur where there is an accumulation of unfolded polypeptides. For this reason, BiP has been identified as a marker for various disease states that are associated with secretory and transmembrane protein misfolding.

General readings

Wang Q, Shu R, et al. (2012). Int J Oncol. 41(2):652-60. Sokolowska I, Woods AG, et al. (2012). FEBS J. 279(14):2579-94.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HSPA5 / GRP78 antibody

Western blot analysis of extracts from COS7 cells, using GRP78 antibody (#TA312902).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPA5 Rabbit Polyclonal antibody. (TA590608) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 (24-43) antibody

Human Breast cancer stained with Anti-GRP78, cat# AP15587PU Rabbit Purified Hu, Ms, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

HSPA5 / GRP78 (24-43) antibody

Human Breast cancer stained with Anti-GRP78, cat# AP15587PU Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €520.00
  Acris Antibodies GmbH

HSPA5 / GRP78 (24-43) antibody

Human Breast cancer stained with Anti-GRP78, cat# AP15587PU Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

HSPA5 / GRP78 antibody

Western blot analysis of GRP78 in (from L-R): Recombinant protein, human, dog and mouse tissues using a 1:1000 dilution of TA309341. Rabbit Purified Bov, Hst, Hu, Mky, Ms, Rb, Rt, Xen WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

Western blot analysis of GRP78 in (from L-R): Recombinant protein, human, dog and mouse tissues using a 1:1000 dilution of TA309344. Rabbit Purified Bov, Hst, Hu, Mky, Ms, Rb, Rt, Xen WB
50 µl / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

Western blot analysis of GRP78 in Glia GD cell lysates using a 1:1000 dilution of the antibody Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

WB analysis of Grp78 using the antibody Mouse IgG2b 1H11-1H7 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

0.5 µg/ml of TA309343 was sufficient for detection of Grp78 in 10ug of HeLa cell lysate by ECL immunoblot analysis. Mouse IgG1 6H4.2G7 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

HSPA5 / GRP78 Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

Western blot analysis of extracts of various cell lines, using BIP antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-mouse BiP antibody. Proteins were visualized using a goat anti-rat secondary conjugated to HRP and a chemiluminescence system. Rat IgG2a 76-E6 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

Simple Western: GRP78 Antibody [TA336638] - Simple Western lane view shows a specific band detected for GRP78 in HeLa lysate.  This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa). Rabbit Purified Hu, Ms, Prim, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

Western Blot: GRP78 Antibody [TA336718] - Detection of Bip/Grp78 on HeLa whole cell extracts using TA336718. Rabbit Purified Chk, Hu, Ms, Rt, Sh F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

Simple Western: GRP78 Antibody [TA336719] - Simple Western lane view shows a specific band detected for GRP78 in HeLa lysate.  This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa). Rabbit Purified Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

Western blot detection of BiP/GRP78 (C-terminus) in Hela,C6,Lncap and MDA-MB-468 cell lysates using BiP/GRP78 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:78KDa. Mouse IgG1 9E4-2A7-H6 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

HSPA5 / GRP78 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA5 / GRP78 antibody

Immunohistochemical analysis of paraffin-embedded human Thyroid tissues using HSPA5 mouse mAb Mouse IgG1 4E3 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

HSPA5 / GRP78 antibody

Figure 1. Western Blot using AM08058BT-N antibody. Recombinant hamster BiP mutant HCB1- (Lane 1), Full-length recombinant hamster BiP (Lane 2) and Total cell lysate from Ag8.653, a mouse myeloma cell line (Lane 3) were resolved by a 10% SDS-PAGE. The proteins were transferred onto a PVDF membrane and incubated with Rat anti-BiP-Biotin followed by Streptavidin-HRP. The blot was then developed using ECL Western blot detection reagents (Amersham Life Science, Arlington Heights, IL). Note: We are grateful to We are grateful to Dr. Linda Hendershot for the recombinant hamster BiP proteins and for helpful suggestions. Rat IgG1 76-E6 Biotin Ms E, FN, WB
0.5 mg / €410.00
  Acris Antibodies GmbH

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