HSPA8 / HSC70 antibody

Principal name

HSPA8 / HSC70 antibody

Alternative names for HSPA8 / HSC70 antibody

HSP73, HSPA10, Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8

SwissProt ID

A2Q0Z1 (Horse), O73885 (Chick), P11142 (Human), P19120 (Bovin), P63017 (Mouse), P63018 (Rat), Q90473 (Danre)

Gene ID

3312 (HSPA8), 24468 (Hspa8), 15481

Available reactivities

Bov (Bovine), Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Por (Porcine), Rt (Rat), Sh (Sheep), Mam (Mammalia), Salmonid, Fi (Fish), Ce (C. elegans), Can (Canine), Chk (Chicken), Dros (Drosophila), GP (Guinea Pig), Pl (Plant), Rb (Rabbit), Xen (Xenopus), Av (Birds), Arabidopsis, Ye (Yeast)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C), Immunoelectron Microscopy (EM), Functional assay (FN)

Background of HSPA8 / HSC70 antibody

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eucaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eucaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5).
All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

General readings

Tsukahara F., et al. (2000) Mol Pharmacol. 58(6):1257-63.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Heat shock protein 70 / HSP70 (+HSP73) antibody

IHC Image for AP22892PU-N
Spleen, neutrophils, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Bov, Can, Dros, Fi, Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

HSP70/ HSC70 antibody

  Mouse monoclonal Hsp70/Hsc70 Antibody  
Western Blot analysis of Hsp70/Hsc70 in human cell line lysates at 1:1000 dilution of the antibody Mouse IgG1 N27F3-4 Purified Beluga, Bov, Can, Ce, Chk, Dros, Fi, GP, Hst, Hu, Mky, Ms, Pl, Por, Rb, Rt, Sh, Xen C, EM, F, IP, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

  Mouse Monoclonal anti-Hsc70 Antibody  
Western blot analysis of Hsp70/Hsc70 in Hela cell lysates using a 1:1000 dilution of anti-Hsc70. Mouse IgG2a BB70 Aff - Purified Artemia, Beluga, Bov, Can, Chk, Dros, Fi, GP, Hst, Hu, Ms, pacific oyster, Por, Rb, Rt, scallop, Sh, Xen, Ye C, ICC/IF, IP, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

  Mouse Monoclonal anti-Hsc70 Antibody  
Western blot analysis of Hsp70/Hsc70 in Hela cell lysates using a 1:1000 dilution of anti-Hsc70. Mouse IgG2a BB70 Aff - Purified Artemia, Beluga, Bov, Can, Chk, Dros, Fi, GP, Hst, Hu, Ms, pacific oyster, Por, Rb, Rt, scallop, Sh, Xen, Ye C, ICC/IF, IP, P, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

  HSPA8 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505966) Mouse IgG3 OTI1H3 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

HSPA8 / HSC70 (Isoform 1) antibody

  Goat Polyclonal Antibody against HSPA8 (Isoform 1)  
TA302666 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

  Mouse Monoclonal anti-HSPA8 Antibody  
Western lot analysis of 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein. Mouse IgG2a 1F2-H5 Purified Hu, Ms, Rt C, E, IP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

  Rabbit polyclonal HSPA8 antibody  
Western blot analysis of extracts from HuvEC, K562, Jurkat and HeLa cells, using HSPA8 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

  Mouse monoclonal anti-HSPA8(HSC70) antibody, clone 1F2-H5, Loading control  
Western lot analysis of 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein. Mouse IgG2a 1F2-H5 Purified Hu, Ms, Rt C, E, IP, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 (618-637) antibody

  Rabbit polyclonal Hsc70 (Hsp73) Antibody  
Western blot analysis of Hsc70 in a human cell line mix, using a 1:1000 dilution of the antibody Rabbit Serum Hst, Hu, Rt C, FN, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

  Rabbit polyclonal anti-HSPA8(HSC70) antibody, Loading control  
Western blot analysis of extracts of various cell lines, using HSPA8 antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 Control Peptide

HSPA8 / HSC70 Purified
50 µg / €190.00
  Acris Antibodies GmbH

HSPA8 / HSC70 antibody

AP00035PU-N 
Western blot analysis of Hsc70
expression in Jurkat cell lysate (Lane 1) and mouse small intestine tissue lysate (Lane 2). Rabbit Purified Bov, Hst, Hu, Mky, Ms, Por, Rt, Sh P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

HSPA8 - human gene knockout kit via CRISPR

HSPA8 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

HSPA8 - human gene knockout kit via CRISPR

HSPA8 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

HSPA8 - human gene knockout kit via CRISPR

HSPA8 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

HSPA8 - human gene knockout kit via CRISPR

HSPA8 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

HSPA8 / HSC70 (transcript variant 1)

  Recombinant protein of human heat shock 70kDa protein 8 (HSPA8), transcript variant 1  
HSPA8 / HSC70 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 (active)

HSPA8 / HSC70 Human Aff - Purified > 90 % pure as determined by SDS-PAGE analysis. E. coli
2x0.1 mg / €570.00
  Acris Antibodies GmbH

HSPA8 / HSC70 (active)

HSPA8 / HSC70 Human Aff - Purified > 90 % pure as determined by SDS-PAGE analysis. E. coli
0.1 mg / €340.00
  Acris Antibodies GmbH

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