HSPA8 / HSC70 antibody

Principal name

HSPA8 / HSC70 antibody

Alternative names for HSPA8 / HSC70 antibody

HSP73, HSPA10, Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8

SwissProt ID

A2Q0Z1 (Horse), O73885 (Chick), P11142 (Human), P19120 (Bovin), P63017 (Mouse), P63018 (Rat), Q90473 (Danre)

Gene ID

3312 (HSPA8), 24468 (Hspa8), 6573376 (Dmoj\GI23058)

Available reactivities

Bov (Bovine), Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Por (Porcine), Rt (Rat), Sh (Sheep), Mam (Mammalia), Salmonid, Fi (Fish), Can (Canine), GP (Guinea Pig), Rb (Rabbit), Fe (Feline), Ce (C. elegans), Chk (Chicken), Dros (Drosophila), Pl (Plant), Xen (Xenopus), Av (Birds), Arabidopsis, Ze (Zebrafish), Ye (Yeast)

Available hosts

Rabbit, Goat, Rat, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Enzyme Immunoassay (E), Neutralisation (NEUT), Frozen Sections (C), Immunoelectron Microscopy (EM), Functional assay (FN)

Background of HSPA8 / HSC70 antibody

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eucaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eucaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5).
All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

General readings

This product has been used in:Maekawa M et al. Heat-shock cognate protein (hsc71) and related proteins in mouse spermatogenic cells. Biol Reprod 40:843-52 (1989). PubMed PMID: 2665832
General / background references:Leung SM & Hightower LE A 16-kDa protein functions as a new regulatory protein for Hsc70 molecular chaperone and is identified as a member of the Nm23/nucleoside diphosphate kinase family. J Biol Chem 272:2607-14 (1997). PubMed PMID: 9006893
Terlecky SR et al. Protein and peptide binding and stimulation of in vitro lysosomal proteolysis by the 73-kDa heat shock cognate protein. J Biol Chem 267:9202-9 (1992). PubMed PMID: 1577755
Gething MJ & Sambrook J Protein folding in the cell. Nature 355:33-45 (1992). PubMed PMID: 1731198
Allen RL et al. A novel hsp70-like protein (P70) is present in mouse spermatogenic cells. Mol Cell Biol 8:828-32 (1988). PubMed PMID: 3352605

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Heat shock protein 70 / HSP70 (+HSP73) antibody

IHC Image for AP22892PU-N
Spleen, neutrophils, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Bov, Can, Dros, Fi, Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

HSP70/ HSC70 antibody

Western Blot analysis of Hsp70/Hsc70 in human cell line lysates at 1:1000 dilution of the antibody Mouse IgG1 N27F3-4 Purified Beluga, Bov, Can, Ce, Chk, Dros, Fi, GP, Hst, Hu, Mky, Ms, Pl, Por, Rb, Rt, Sh, Xen C, EM, F, IP, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

HSP70/ HSC70 antibody

Western Blot analysis of Hsp70/Hsc70 in human cell line lysates at 1:1000 dilution of the antibody Mouse IgG1 N27F3-4 Purified Beluga, Bov, Can, Ce, Chk, Dros, Fi, GP, Hst, Hu, Mky, Ms, Pl, Por, Rb, Rt, Sh, Xen C, EM, F, ICC/IF, IP, WB
50 µg / €230.00
  Acris Antibodies GmbH

HSPA8 / HSC70 antibody

Western blot analysis of Hsp70/Hsc70 in Hela cell lysates using a 1:1000 dilution of anti-Hsc70. Mouse IgG2a BB70 Aff - Purified Artemia, Beluga, Bov, Can, Chk, Dros, Fi, GP, Hst, Hu, Ms, pacific oyster, Por, Rb, Rt, scallop, Sh, Xen, Ye C, ICC/IF, IP, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

Western blot analysis of Hsp70/Hsc70 in Hela cell lysates using a 1:1000 dilution of anti-Hsc70. Mouse IgG2a BB70 Aff - Purified Artemia, Beluga, Bov, Can, Chk, Dros, Fi, GP, Hst, Hu, Ms, pacific oyster, Por, Rb, Rt, scallop, Sh, Xen, Ye C, ICC/IF, IP, P, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505966) Mouse IgG3 OTI1H3 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

HSPA8 / HSC70 antibody

Human Pineal Tissue; Rabbit Anti-HSPA8 Antibody . Catalog Number: ARP48445_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue . Observed Staining: Nuclear in pinealocytes . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-HSPA8 Antibody (ARP48445_P050) ) in Human Pineal Tissue using Immunohistochemistry Rabbit Aff - Purified Arabidopsis, Can, Ce, Dros, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

HSPA8 / HSC70 antibody

Human Brian; Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science. Application: IHC. Species+tissue/cell type: Human brain stem cells. Primary antibody dilution: 1:500. Secondary antibody: Goat anti-rabbit Alexa-Fluor 594. Secondary antibody dilution: 1:1000; HSPA8 antibody - N-terminal region (ARP48446_P050) in Human Brian cells using Immunohistochemistry Rabbit Aff - Purified Arabidopsis, Can, Ce, Dros, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

HSPA8 / HSC70 (Isoform 1) antibody

TA302666 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

HSPA8 / HSC70 (Isoform 1) antibody

TA302666 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

Western lot analysis of 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein. Mouse IgG2a 1F2-H5 Purified Hu, Ms, Rt C, E, IP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

Western blot analysis of extracts from HuvEC, K562, Jurkat and HeLa cells, using HSPA8 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

Western lot analysis of 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein. Mouse IgG2a 1F2-H5 Purified Hu, Ms, Rt C, E, IP, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 (618-637) antibody

Western blot analysis of Hsc70 in a human cell line mix, using a 1:1000 dilution of the antibody Rabbit Serum Hst, Hu, Rt C, FN, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 antibody

Western blot analysis of extracts of various cell lines, using HSPA8 antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPA8 / HSC70 Control Peptide

HSPA8 / HSC70 Purified
50 µg / €190.00
  Acris Antibodies GmbH

HSPA8 / HSC70 antibody

AP00035PU-N 
Western blot analysis of Hsc70
expression in Jurkat cell lysate (Lane 1) and mouse small intestine tissue lysate (Lane 2). Rabbit Purified Bov, Hst, Hu, Mky, Ms, Por, Rt, Sh P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

HSPA8 / HSC70 (618-637) antibody

Western blot analysis of Hsc70 in a human cell line mix, using a 1:1000 dilution of the antibody Rabbit Serum Hst, Hu, Rt C, FN, ICC/IF, IP, P, WB
25 µl / €210.00
  Acris Antibodies GmbH

HSPA8 / HSC70 antibody

HSPA8 / HSC70 Mouse IgG2a 1G2 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HSPA8 / HSC70 antibody

Western blot using HSPA8 monoclonal antibody, clone C92F3A-5 ( Cat # MAB1923 ) shows detection of HSPA8 in whole cell lysates from heat shocked HeLa cells.
The band marked by the arrowhead corresponds to HSPA8 at an approximate molecular weight of 70 kDa.
The primary antibody was used at a 1:1000 dilution. Bov, Chk, GP, Hst, Hu, Mky, Ms, Rb, Rt, Sh
0.1 mg / €560.00
  Abnova Taiwan Corp.

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