HSPB1 / HSP27 antibody
Principal name
HSPB1 / HSP27 antibody
Alternative names for HSPB1 / HSP27 antibody
Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein
SwissProt ID
P04792 (Human), P14602 (Mouse), P42929 (Canfa), P42930 (Rat), Q00649 (Chick), Q3T149 (Bovin), Q5S1U1 (Pig)
Ncbi ID
NP_001531.1, NP_001531, 9606, NP_114176.3, NM_013560, NM_031970, NM_001540, NP_038588.2
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Dot blot (Dot)
Background of HSPB1 / HSP27 antibody
Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5).
HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction.
Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).
General readings
1. Slotta-Huspenina J et al. A specific expression profile of heat-shock proteins and glucose-regulated proteins is associated with response to neoadjuvant chemotherapy in oesophageal adenocarcinomas. Br J Cancer 109:370-8 (2013).PubMed: 23839491
2. Martínez-Laorden E et al. Morphine withdrawal activates hypothalamic-pituitary-adrenal axis and heat shock protein 27 in the left ventricle: the role of extracellular signal-regulated kinase. J Pharmacol Exp Ther 342:665-75 (2012). WB ; Rat .PubMed: 22647273
3. Hsiao CY et al. A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine, Angelica sinensis, Using a Proteomic Approach. Evid Based Complement Alternat Med 2012:467531 (2012). WB ; Human .PubMed: 22536285
4. Almela P et al. Naloxone-precipitated morphine withdrawal evokes phosphorylation of heat shock protein 27 in rat heart through extracellular signal-regulated kinase. J Mol Cell Cardiol51:129-39 (2011). WB ; Rat .PubMed: 21530534
5. Berg D et al. Discovery of new molecular subtypes in oesophageal adenocarcinoma. PLoS One 6:e23985 (2011). Human .PubMed: 21966358
HSP 27 activation Serum starved HepG2 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). Immunoblots were probed with mab hsp27-5B9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1h EGFLane 6; 2h EGF Lane 7: 4h EGFLane 8: 8h EGF
Western blot analysis of Hsp25/27 in rat lung tissues, using a 1:1000 dilution of the antibody
Somites of a rat embryo at 11 days of gestation. Red is rhodamine-phalloidin labeled actin and green is Hsp27. Courtesy of Mike Welsh, University of Michigan.
Formalin-Fixed Paraffin-Embedded Human Skeletal muscle stained with HSPB1 / HSP27 Antibody Cat.-No AM08290PU-N at 25 µg/ml after heat-induced antigen retrieval
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp27 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Immunohistochemistry: Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp27 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and Diaminobenzidine (DAB) was used for detection.
Western blot analysis on HeLa cells: Cell lysates of HeLa (each 30 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of HeLa(each 30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
FACS analysis. Anti-Hsp270-FITC staining on heat shock treated CD3+CD8+T cells.
Immunohistochemistry (IHC) analyzes of HSP27 pSer15 antibody (Cat.-No.: AP01607PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of HSP27 pSer15 antibody (Cat.-No.: AP01607PU-N) in extracts from 293 cells treated with UV.
Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HSP27 (phospho-Ser15) antibody AP02446PU.
Figure 2. Western blot analysis of extract from HeLa cells using HSP27 antibody (Lane1 and 2) and HSP27 (phospho-Ser15) antibody AP02446PU (Lane 3 and 4).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSP27 antibody (AP02500PU-S; AP02500PU-N).
Figure 3 Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (pSer78) antibody
Figure 4 Western blot analysis of extracts from HL60 cells untreated or treated wit UV using HSP27 (pSer78) antibody
Western blot analysis of extracts from HeLa cells using HSP27 antibody (Lane 1 and 2) and HSP27 (pSer78) antibody (AP02500PU-S; AP02500PU-N, Lane 3 and 4).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 antibody (AP02501PU-S; AP02501PU-N).
Western blot analysis of extracts from Hela cells untreated or treated with Heat Shock or UV, using HSP27 antibody (Lane 1, 2 and 3) and HSP27 (PSer82) antibody (AP02501PU-S; AP02501PU-N, Lane 4, 5 and 6).
Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HSP27 antibody AP02671PU.
Figure 2. Western blot analysis of extracts from HeLa cells using HSP27 antibody AP02671PU (Lane1 and 2) and HSP27 (phospho-Ser15) antibody AP02446PU (Lane 3 and 4).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSP27 Antibody Cat.-No AP02743PU
Western blot analysis of extracts from HeLa cells using HSP27 Antibody Cat.-No AP02743PU
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 Antibody Cat.-No AP02744PU
Western blot analysis of extracts from K562 and Hela cells using HSP27 Antibody Cat.-No AP02744PU
Immunohistochemistry (IHC) analyzes of HSP27 antibody (Cat.-No.: AP06172PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of HSP27 antibody (Cat.-No.: AP06172PU-N)in extracts from HeLa cells.
Dot blot analysis of Phospho-HSBP1-S78 polyclonal antibody (Cat#AP12798PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.
Western blot analysis of HSP27 antibody (Center) (AP17488PU-N) in MCF-7 cell line lysates (35ug/lane). HSP27 (arrow) was detected using the purified Pab.
Immunohistochemistry (IHC) analyzes of HSP27 antibody (Cat.-No.: AP20565PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analysis of HSP27 antibody (Cat.-No.: AP20565PU-N) in extracts from sp20 cells.
Immunohistochemistry analyzes of HSP27 antibody (Cat.-No.: AP20728PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot analyzes of HSP27 antibody (Cat.-No.: AP20728PU-N) in extracts from Jurkat cells.
Western blot (WB) analyzes of p-HSP27 antibody (Cat.-No.: AP20847PU-N) in extracts from HeLa Ca2+ cells.
Immunohistochemistry (IHC) analyzes of p-HSP27 antibody (Cat.-No.: AP20848PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot (WB) analyzes of p-HSP27 antibody (Cat.-No.: AP20848PU-N) in extracts from HUVEC TNF cells.
Immunohistochemistry (IHC) analyzes of p-HSP27 antibody (Cat.-No.: AP20950PU-N) in paraffin-embedded human breast carcinoma tissue.
Immunohistochemistry (IHC) analyzes of HSP27 pAb in paraffin-embedded human breast carcinoma tissue.
Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with Heat Shock Protein 27 (HSP27) antibody AP22766PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Heart (formalin-fixed, paraffin-embedded) stained with Heat Shock Protein 27 (HSP27) antibody AP22766PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
HSP27 Polyclonal Antibody
Recombinant human HSP27 (1-205 aa), His-tagged: 15% SDS-PAGE (3 µg)
Human kidney; HSPB1 antibody - C-terminal region (ARP30177_T100) in Human kidney cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-HSPB1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; HSPB1 antibody - C-terminal region (ARP30177_T100) in Human HepG2 cells using Western Blot
Human HepG2; WB Suggested Anti-HSPB1 Antibody Titration: 1.0ug/ml. Positive Control: HepG2 cell lysate; HSPB1 antibody - C-terminal region (AVARP09060_T100) in Human HepG2 cells using Western Blot
Proximity Ligation Analysis (PLA) of protein-protein interactions between HSPB1 and F13A1. Huh7 cells were stained with anti-HSPB1 rabbit purified polyclonal 1:1200 and anti-F13A1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Proximity Ligation Analysis (PLA) of protein-protein interactions between HSPB1 and EGFR. HeLa cells were stained with anti-HSPB1 rabbit purified polyclonal 1:1200 and anti-EGFR mouse purified polyclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
HSPB1 MaxPab rabbit polyclonal antibody. Western Blot analysis of HSPB1 expression in human liver.
Western Blot analysis of HSPB1 expression in transfected 293T cell line (H00003315-T01) by HSPB1 MaxPab polyclonal antibody.Lane 1: HSPB1 transfected lysate(22.80 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and HSPB1. Mahlavu cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-HSPB1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Detection limit for recombinant GST tagged HSPB1 is approximately 0.1ng/ml as a capture antibody.
Western blot analysis of HeLa cell lysate ( each 30 µg ) was resolved by SDS - PAGE , transferred to PVDF membrane and probed with HSPB1 monoclonal antibody , clone 2A5 ( 1 : 1000 ) ( Cat # MAB1091 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunohistochemical staining using HSPB1 monoclonal antibody, clone G3.1 on formalin-fixed, paraffin-embedded human breast carcinoma with peroxidase-conjugate and DAB chromogen.
HSPB1 (phospho S78) polyclonal antibody ( Cat # PAB0490 ). Western blot analysis of HSPB1 expression in MCF-7.
HSPB1 (phospho S78) polyclonal antibody ( Cat # PAB0490 ). Western blot analysis of HSPB1 expression in human stomach.
Dot blot analysis of HSPB1 (phospho S78) polyclonal antibody ( Cat # PAB0490 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5 µg/ml. P-Pab : phospho-antibody; P-Peptide : phospho-peptide; NP-Peptide : non-phospho-peptide.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HSPB1 polyclonal antibody ( Cat # PAB2047 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of HSPB1 ( arrow ) using HSPB1 polyclonal antibody ( Cat # PAB2047 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the HSPB1 gene ( Lane 2 ) ( Origene Technologies ) .
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HSPB1 polyclonal antibody ( Cat # PAB2048 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of HSPB1 ( arrow ) using HSPB1 polyclonal antibody ( Cat # PAB2048 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the HSPB1 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of HSPB1 ( arrow ) using HSPB1 polyclonal antibody ( Cat # PAB2761 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the HSPB1 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of HSPB1 polyclonal antibody ( Cat # PAB2761 ) in Hela cell line lysates ( 35 µg/lane ) . HSPB1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Imunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSPB1 polyclonal antibody ( Cat # PAB7676 ).
Western blot analysis of extracts from HeLa cells treated with heat shock or UV, using HSPB1 polyclonal antibody ( Cat # PAB7676 ).
Human Skin, Epidermis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts from HeLa cells, treated with Ca2+, using HSP27 Antibody. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using HSP27 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and cardiac muscle tissues (right) using HSP27 mouse mAb with DAB staining.
Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate.
Red: Control Antigen (100ng);Purple: Antigen (10ng);Green: Antigen (50ng);Blue: Antigen (100ng);
Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and negative control (purple).
Western blot analysis of Hspb1 polyclonal antibody expression in rat kidney tissue lysate.
Formalin-Paraffin breast carcinoma (20X) stained with HSP27 Antibody Cat.-No AM50114PU (Clone SPM252).
Formalin-Paraffin breast carcinoma (10X) stained with HSP27 Antibody Cat.-No AM50114PU (Clone SPM252).
Formalin-Paraffin breast carcinoma (10X) stained with HSP27 Antibody Cat.-No AM33370PU (Clone G3.1).
Formalin-Paraffin breast carcinoma (20X) stained with HSP27 Antibody Cat.-No AM33370PU (Clone G3.1).
Immunohistochemical analysis of HSP27 (pS15) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of HSP27 (pS15) expression in HeLa Anisomycin-treated (A); MCF7 (B) whole cell lysates.
Representative Standard Curve
Immunohistochemical analysis of HSP27 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of HSP27 expression in HeLa (A); HepG2 (B) whole cell lysates.
Western blot analysis of HSP27 expression in HeLa (A); Jurkat (B) whole cell lysates.
Immunohistochemical analysis of HSP27 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Detection of CryAB (0.5ug/ml). Lane 1: Human skeletal muscle. Lane 2: Mouse skeletal muscle
Detection of CryAB (0.5ug/ml). Lane 1: Human skeletal muscle. Lane 2: Mouse skeletal muscle
Predicted band size: 23 kDa . Positive control: Hela cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HSPB1 (Ab-78) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
Western blot analysis of HSP27 expression in Ca2+ treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western Blot: Hsp27 Antibody (6H11) [TA337102] - HeLa cell crude extracts. Lane 16 was probed with TA337102, while lanes 14 and 15 were probed with two other monoclonals to Hsp27. Note the strong clean bands at 27 kDa. Lane 17 was probed with NBP2-25151,
Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [TA337102] - HeLa cells stained with TA337102 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). TA337102 reveals strong cytoplasmic staini
Flow Cytometry: Hsp27 Antibody (6H11) [TA337102] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Western Blot: Hsp27 Antibody [TA337103] - Blot of HeLa cell crude extracts. Note the strong clean bands at 27kDa corresponding to Hsp27.
Immunocytochemistry/Immunofluorescence: Hsp27 Antibody [TA337103] - HeLa cells stained with TA337103 (green), and counterstained with monoclonal antibody to HMGB1 (NBP2-25148, red) and DNA (blue). TA337103 reveals strong cytoplasmic staining and penetrate
Western blot analysis of HSPB1 (arrow) using rabbit polyclonal HSPB1 Antibody (S78) (RB11380). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HSPB1 Antibody (S78) (Cat.#TA302047), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of HSPB1 (arrow) using rabbit polyclonal HSPB1 Antibody (S82) (RB12317). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HSPB1 Antibody (S82) (Cat.#TA302049), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of HSPB1 Antibody (S83) (Cat.#TA302050) in Hela cell line lysates (35ug/lane). HSPB1 (arrow) was detected using the purified Pab.
Western blot analysis of HSPB1(arrow) using rabbit polyclonal HSPB1 Antibody (S83) (Cat.#TA302050). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2) (Origene Technologies).
Western blot analysis of extracts of HUVEC cells, using HSPB1 antibody.
Western Blot: Hsp27 Antibody [TA336819] - Analysis of HSP27 in human skeletal muscle lysate.
Western Blot: Hsp27 Antibody [TA336819] - analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.
Immunohistochemistry: Hsp27 Antibody [TA336819] - Staining of HSP27 in mouse heart using DAB with hematoxylin counterstain.
Immunocytochemistry/Immunofluorescence: Hsp27 Antibody [TA336819] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Western blot detection of Hsp27 in Hela,HsHela,A431,COS7,Lncap and MCF7 cell lysates and using Hsp27 mouse mAb (1:1000 diluted).Predicted band size: 23KDa.Observed band size:27KDa.
Immunocytochemistry stain of Hela using Hsp27 mouse mAb (1:300).
Western blot analysis of HSP27 expression in Hela (A) whole cell lysates.
Immunohistochemical analysis of HSP27 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of HSP27 expression in Hela (A); C2C12 (B); mouse brain (C); rat brain (D) whole cell lysates.
Immunohistochemical analysis of HSP27 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of HSP27 phosphorylation expression in Ca2+ treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of HSP27 phosphorylation expression in Ca2+ treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of HSP27 phosphorylation expression in UV treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Predicted band size: 23 kDa . Positive control: HL60 cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: HL60 cells untreated with UV lysate Lane2: HL60 cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HSPB1 (Phospho-Ser82) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
Predicted band size: 23 kDa . Positive control: HL60 cells treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: HL60 cells untreated with UV lysate Lane2: HL60 cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HSPB1 (Phospho-Ser78) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasmic, Nuclear and centrosomal. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using HSPB1 (Phospho-Ser78) antibody at dilution 1/100. (Original magnification:×200)
Predicted band size: 23 kDa . Positive control: Hela cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: Hela cells untreated with UV lysate Lane2: Hela cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HSPB1 (Phospho-Ser15) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm; Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200 The image is immunofluorescence of methanol-fixed Hela cells using HSPB1 (Phospho-Ser15) antibody at dilution 1/100. (Original magnification:×200)
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA336819 | HSPB1 / HSP27 antibody |
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| Rabbit Polyclonal HSP27 Antibody | |||||||
![Western Blot: Hsp27 Antibody [TA336819] - Analysis of HSP27 in human skeletal muscle lysate.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-75477-1-W.jpg) |
Rabbit | Purified | Hu, Ms, Rt | C, ICC/IF, P, WB |
0.1 ml /
€393.00
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| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| AM06625SU-N | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | 5D7 | Ascites | Hu, Rt | E, ICC/IF, P, WB |
0.1 ml /
€430.00
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| Acris Antibodies GmbH | |||||||
| +5 additional images |
Highly validated antibody! |
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| AM09016PU-N | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | 2A5 | Purified | Hu | E, P, WB |
0.1 ml /
€310.00
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| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AM09016PU-S | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | 2A5 | Purified | Hu | E, P, WB |
50 µl /
€230.00
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| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AP02500PU-N | HSPB1 / HSP27 pSer78 antibody |
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Rabbit | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 mg /
€320.00
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| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AP02500PU-S | HSPB1 / HSP27 pSer78 antibody |
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Rabbit | Aff - Purified | Hu | ICC/IF, P, WB |
50 µg /
€240.00
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| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AM33370PU-N | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | G3.1 | Purified | Chimp, Chk, Hu, Mky, Ms, Rt, Sh | C, F, ICC/IF, IP, P, WB |
0.2 mg /
€410.00
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
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| AM33370PU-S | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | G3.1 | Purified | Chimp, Chk, Hu, Mky, Ms, Rt, Sh | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€320.00
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
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| AM33370PU-T | HSPB1 / HSP27 antibody |
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|
Mouse | IgG1 | G3.1 | Purified | Chimp, Chk, Hu, Mky, Ms, Rt, Sh | C, F, ICC/IF, IP, P, WB |
20 µg /
€180.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
||||||
| AM50114PU-N | HSPB1 / HSP27 antibody |
||||||
 |
Mouse | IgG1 | SPM252 | Purified | Chimp, Chk, Hu, Ms, Rt, Sh | C, ICC/IF, IP, P, WB |
0.2 mg /
€410.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
||||||
| AM50114PU-S | HSPB1 / HSP27 antibody |
||||||
|
Mouse | IgG1 | SPM252 | Purified | Chimp, Chk, Hu, Ms, Rt, Sh | C, ICC/IF, IP, P, WB |
0.1 mg /
€320.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
||||||
| AM50114PU-T | HSPB1 / HSP27 antibody |
||||||
|
Mouse | IgG1 | SPM252 | Purified | Chimp, Chk, Hu, Ms, Rt, Sh | C, ICC/IF, IP, P, WB |
20 µg /
€180.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
||||||
| TA301439 | HSPB1 / HSP27 antibody |
||||||
| Rabbit Polyclonal Antibody against Crystallin AB | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€416.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA301440 | HSPB1 / HSP27 antibody |
||||||
| Rabbit Polyclonal Antibody against Crystallin AB | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€393.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA302047 | HSPB1 / HSP27 antibody |
||||||
| Rabbit Polyclonal Antibody against HSPB1 (S78) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€410.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA302049 | HSPB1 / HSP27 antibody |
||||||
| Rabbit Polyclonal Antibody against HSPB1 (S82) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€410.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA302050 | HSPB1 / HSP27 antibody |
||||||
| Rabbit Polyclonal Antibody against HSPB1 (S83) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€410.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA309363 | HSPB1 / HSP27 antibody |
||||||
| Mouse Monoclonal anti-HSPB1 Antibody | |||||||
 |
Mouse | IgG2b | 5D12-A12 | Purified | Hu | E, IP, P, WB |
50 µg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| TA309366 | HSPB1 / HSP27 antibody |
||||||
| Mouse Monoclonal anti-HSPB1 Antibody | |||||||
 |
Mouse | IgG2b | 5D12-A12 | FITC | Hu | F |
0.2 mg /
€433.00
|
| OriGene Technologies, Inc. | |||||||
| TA321366 | HSPB1 / HSP27 antibody |
||||||
| Anti-HSPB1 (Phospho-Ser15) Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
