HSPB1 / HSP27 antibody

Principal name

HSPB1 / HSP27 antibody

Alternative names for HSPB1 / HSP27 antibody

Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein

SwissProt ID

P04792 (Human), P14602 (Mouse), P42929 (Canfa), P42930 (Rat), Q00649 (Chick), Q3T149 (Bovin), Q5S1U1 (Pig)

Gene ID

3315 (HSPB1), 24471 (Hspb1), 15507

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), GP (Guinea Pig), Hst (Hamster), Mam (Mammalia), Tr (Turkey), Mky (Monkey), Chk (Chicken), Fe (Feline), Fi (Fish), Gt (Goat), Por (Porcine), Chimp (Chimpanzee), Sh (Sheep)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Dot blot (Dot)

Background of HSPB1 / HSP27 antibody

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5).
HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction.
Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

General readings

1. Slotta-Huspenina J  et al. A specific expression profile of heat-shock proteins and glucose-regulated proteins is associated with response to neoadjuvant chemotherapy in oesophageal adenocarcinomas. Br J Cancer 109:370-8 (2013).PubMed: 23839491
2. Martínez-Laorden E  et al. Morphine withdrawal activates hypothalamic-pituitary-adrenal axis and heat shock protein 27 in the left ventricle: the role of extracellular signal-regulated kinase. J Pharmacol Exp Ther 342:665-75 (2012). WB ; Rat .PubMed: 22647273
3. Hsiao CY  et al. A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine, Angelica sinensis, Using a Proteomic Approach. Evid Based Complement Alternat Med 2012:467531 (2012). WB ; Human .PubMed: 22536285
4. Almela P  et al. Naloxone-precipitated morphine withdrawal evokes phosphorylation of heat shock protein 27 in rat heart through extracellular signal-regulated kinase. J Mol Cell Cardiol51:129-39 (2011). WB ; Rat .PubMed: 21530534
5. Berg D  et al. Discovery of new molecular subtypes in oesophageal adenocarcinoma. PLoS One 6:e23985 (2011). Human .PubMed: 21966358

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HSPB1 / HSP27 antibody

  Rabbit Polyclonal HSP27 Antibody  
Western Blot: Hsp27 Antibody [TA336819] - Analysis of HSP27 in human skeletal muscle lysate. Rabbit Purified Hu, Ms, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPB1 / HSP27 antibody

Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using HSP27 mouse mAb with DAB staining. Mouse IgG1 5D7 Ascites Hu, Rt E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

HSPB1 / HSP27 antibody

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp27 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG1 2A5 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

HSPB1 / HSP27 antibody

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp27 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG1 2A5 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

HSPB1 / HSP27 pSer78 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSP27 antibody (AP02500PU-S; AP02500PU-N). Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

HSPB1 / HSP27 pSer78 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSP27 antibody (AP02500PU-S; AP02500PU-N). Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

HSPB1 / HSP27 antibody

Formalin-Paraffin breast carcinoma (10X) stained with HSP27 Antibody Cat.-No AM33370PU (Clone G3.1). Mouse IgG1 G3.1 Purified Chimp, Chk, Hu, Mky, Ms, Rt, Sh C, F, ICC/IF, IP, P, WB
0.2 mg / €410.00
  Acris Antibodies GmbH

HSPB1 / HSP27 antibody

Formalin-Paraffin breast carcinoma (10X) stained with HSP27 Antibody Cat.-No AM33370PU (Clone G3.1). Mouse IgG1 G3.1 Purified Chimp, Chk, Hu, Mky, Ms, Rt, Sh C, F, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

HSPB1 / HSP27 antibody

Formalin-Paraffin breast carcinoma (10X) stained with HSP27 Antibody Cat.-No AM33370PU (Clone G3.1). Mouse IgG1 G3.1 Purified Chimp, Chk, Hu, Mky, Ms, Rt, Sh C, F, ICC/IF, IP, P, WB
20 µg / €180.00
  Acris Antibodies GmbH

HSPB1 / HSP27 antibody

Formalin-Paraffin breast carcinoma (20X) stained with HSP27 Antibody Cat.-No AM50114PU (Clone SPM252). Mouse IgG1 SPM252 Purified Chimp, Chk, Hu, Ms, Rt, Sh C, ICC/IF, IP, P, WB
0.2 mg / €410.00
  Acris Antibodies GmbH

HSPB1 / HSP27 antibody

Formalin-Paraffin breast carcinoma (20X) stained with HSP27 Antibody Cat.-No AM50114PU (Clone SPM252). Mouse IgG1 SPM252 Purified Chimp, Chk, Hu, Ms, Rt, Sh C, ICC/IF, IP, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

HSPB1 / HSP27 antibody

Formalin-Paraffin breast carcinoma (20X) stained with HSP27 Antibody Cat.-No AM50114PU (Clone SPM252). Mouse IgG1 SPM252 Purified Chimp, Chk, Hu, Ms, Rt, Sh C, ICC/IF, IP, P, WB
20 µg / €180.00
  Acris Antibodies GmbH

HSPB1 / HSP27 antibody

  Rabbit Polyclonal Antibody against Crystallin AB  
Detection of CryAB (0.5ug/ml). Lane 1: Human skeletal muscle. Lane 2: Mouse skeletal muscle Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPB1 / HSP27 antibody

  Rabbit Polyclonal Antibody against Crystallin AB  
Detection of CryAB (0.5ug/ml). Lane 1: Human skeletal muscle. Lane 2: Mouse skeletal muscle Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HSPB1 / HSP27 antibody

  Rabbit Polyclonal Antibody against HSPB1 (S78)  
Western blot analysis of HSPB1 (arrow) using rabbit polyclonal HSPB1 Antibody (S78) (RB11380). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPB1 / HSP27 antibody

  Rabbit Polyclonal Antibody against HSPB1 (S82)  
Western blot analysis of HSPB1 (arrow) using rabbit polyclonal HSPB1 Antibody (S82) (RB12317). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPB1 / HSP27 antibody

  Rabbit Polyclonal Antibody against HSPB1 (S83)  
Western blot analysis of HSPB1 Antibody (S83) (Cat.#TA302050) in Hela cell line lysates (35ug/lane). HSPB1 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HSPB1 / HSP27 antibody

  Mouse Monoclonal anti-HSPB1 Antibody  
Western blot analysis of Hsp27 in a variety of cells lines using a 1:1000 dilution of the antibody Mouse IgG2b 5D12-A12 Purified Hu E, IP, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HSPB1 / HSP27 antibody

  Mouse Monoclonal anti-HSPB1 Antibody  
FACS analysis. Anti-Hsp270-FITC staining on heat shock treated CD3+CD8+T cells. Mouse IgG2b 5D12-A12 FITC Hu F
0.2 mg / €325.00
  OriGene Technologies, Inc.

HSPB1 / HSP27 antibody

  Anti-HSPB1 (Phospho-Ser15) Rabbit Polyclonal Antibody  
Predicted band size: 23 kDa . Positive control: Hela cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: Hela cells untreated with UV lysate Lane2: Hela cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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