HSPB1 / HSP27 antibody
Principal name
HSPB1 / HSP27 antibody
Alternative names for HSPB1 / HSP27 antibody
Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein
SwissProt ID
P04792 (Human), P14602 (Mouse), P42929 (Canfa), P42930 (Rat), Q00649 (Chick), Q3T149 (Bovin), Q5S1U1 (Pig)
Ncbi ID
NP_001531.1, NP_001531, 9606, NP_114176.3, NM_013560, NM_031970, NM_001540, NP_038588.2
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Dot blot (Dot)
Background of HSPB1 / HSP27 antibody
Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5).
HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction.
Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).
General readings
1. Slotta-Huspenina J et al. A specific expression profile of heat-shock proteins and glucose-regulated proteins is associated with response to neoadjuvant chemotherapy in oesophageal adenocarcinomas. Br J Cancer 109:370-8 (2013).PubMed: 23839491
2. Martínez-Laorden E et al. Morphine withdrawal activates hypothalamic-pituitary-adrenal axis and heat shock protein 27 in the left ventricle: the role of extracellular signal-regulated kinase. J Pharmacol Exp Ther 342:665-75 (2012). WB ; Rat .PubMed: 22647273
3. Hsiao CY et al. A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine, Angelica sinensis, Using a Proteomic Approach. Evid Based Complement Alternat Med 2012:467531 (2012). WB ; Human .PubMed: 22536285
4. Almela P et al. Naloxone-precipitated morphine withdrawal evokes phosphorylation of heat shock protein 27 in rat heart through extracellular signal-regulated kinase. J Mol Cell Cardiol51:129-39 (2011). WB ; Rat .PubMed: 21530534
5. Berg D et al. Discovery of new molecular subtypes in oesophageal adenocarcinoma. PLoS One 6:e23985 (2011). Human .PubMed: 21966358


































































































- Quick-Filters:
- Cited (2)
- Highly Validated (6)
Primary Antibodies
Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
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TA336819 | HSPB1 / HSP27 antibody |
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Rabbit Polyclonal HSP27 Antibody | |||||||
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Rabbit | Purified | Hu, Ms, Rt | C, ICC/IF, P, WB |
0.1 ml /
€393.00
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OriGene Technologies, Inc. | |||||||
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Highly validated antibody! |
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AM06625SU-N | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | 5D7 | Ascites | Hu, Rt | E, ICC/IF, P, WB |
0.1 ml /
€430.00
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Acris Antibodies GmbH | |||||||
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Highly validated antibody! |
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AM09016PU-N | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | 2A5 | Purified | Hu | E, P, WB |
0.1 ml /
€310.00
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Acris Antibodies GmbH | |||||||
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Highly validated antibody! |
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AM09016PU-S | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | 2A5 | Purified | Hu | E, P, WB |
50 µl /
€230.00
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Acris Antibodies GmbH | |||||||
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Highly validated antibody! |
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AP02500PU-N | HSPB1 / HSP27 pSer78 antibody |
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Rabbit | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 mg /
€320.00
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Acris Antibodies GmbH | |||||||
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Highly validated antibody! |
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AP02500PU-S | HSPB1 / HSP27 pSer78 antibody |
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Rabbit | Aff - Purified | Hu | ICC/IF, P, WB |
50 µg /
€240.00
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Acris Antibodies GmbH | |||||||
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Highly validated antibody! |
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AM33370PU-N | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | G3.1 | Purified | Chimp, Chk, Hu, Mky, Ms, Rt, Sh | C, F, ICC/IF, IP, P, WB |
0.2 mg /
€410.00
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Acris Antibodies GmbH | |||||||
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Acris recommends this product for Immunohistochemistry. |
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AM33370PU-S | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | G3.1 | Purified | Chimp, Chk, Hu, Mky, Ms, Rt, Sh | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€320.00
|
Acris Antibodies GmbH | |||||||
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Acris recommends this product for Immunohistochemistry. |
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AM33370PU-T | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | G3.1 | Purified | Chimp, Chk, Hu, Mky, Ms, Rt, Sh | C, F, ICC/IF, IP, P, WB |
20 µg /
€180.00
|
Acris Antibodies GmbH | |||||||
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Acris recommends this product for Immunohistochemistry. |
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AM50114PU-N | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | SPM252 | Purified | Chimp, Chk, Hu, Ms, Rt, Sh | C, ICC/IF, IP, P, WB |
0.2 mg /
€410.00
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Acris Antibodies GmbH | |||||||
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Acris recommends this product for Immunohistochemistry. |
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AM50114PU-S | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | SPM252 | Purified | Chimp, Chk, Hu, Ms, Rt, Sh | C, ICC/IF, IP, P, WB |
0.1 mg /
€320.00
|
Acris Antibodies GmbH | |||||||
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Acris recommends this product for Immunohistochemistry. |
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AM50114PU-T | HSPB1 / HSP27 antibody |
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Mouse | IgG1 | SPM252 | Purified | Chimp, Chk, Hu, Ms, Rt, Sh | C, ICC/IF, IP, P, WB |
20 µg /
€180.00
|
Acris Antibodies GmbH | |||||||
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Acris recommends this product for Immunohistochemistry. |
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TA301439 | HSPB1 / HSP27 antibody |
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Rabbit Polyclonal Antibody against Crystallin AB | |||||||
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Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€416.00
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OriGene Technologies, Inc. | |||||||
TA301440 | HSPB1 / HSP27 antibody |
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Rabbit Polyclonal Antibody against Crystallin AB | |||||||
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Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€393.00
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OriGene Technologies, Inc. | |||||||
TA302047 | HSPB1 / HSP27 antibody |
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Rabbit Polyclonal Antibody against HSPB1 (S78) | |||||||
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€410.00
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OriGene Technologies, Inc. | |||||||
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TA302049 | HSPB1 / HSP27 antibody |
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Rabbit Polyclonal Antibody against HSPB1 (S82) | |||||||
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€410.00
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OriGene Technologies, Inc. | |||||||
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TA302050 | HSPB1 / HSP27 antibody |
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Rabbit Polyclonal Antibody against HSPB1 (S83) | |||||||
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€410.00
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OriGene Technologies, Inc. | |||||||
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TA309363 | HSPB1 / HSP27 antibody |
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Mouse Monoclonal anti-HSPB1 Antibody | |||||||
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Mouse | IgG2b | 5D12-A12 | Purified | Hu | E, IP, P, WB |
50 µg /
€325.00
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OriGene Technologies, Inc. | |||||||
TA309366 | HSPB1 / HSP27 antibody |
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Mouse Monoclonal anti-HSPB1 Antibody | |||||||
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Mouse | IgG2b | 5D12-A12 | FITC | Hu | F |
0.2 mg /
€433.00
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OriGene Technologies, Inc. | |||||||
TA321366 | HSPB1 / HSP27 antibody |
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Anti-HSPB1 (Phospho-Ser15) Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€335.00
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OriGene Technologies, Inc. | |||||||
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