HTRA2 / PRSS25 antibody

Principal name

HTRA2 / PRSS25 antibody

Alternative names for HTRA2 / PRSS25 antibody

OMI, Serine protease 25, mitochondrial Serine protease HTRA2, High temperature requirement protein A2, Omi stress-regulated endoprotease, Serine proteinase OMI

SwissProt ID

A0JNK3 (Bovin), O43464 (Human), Q9JIY5 (Mouse)

Gene ID

27429 (HTRA2), 4027429 (apaH)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Paraffin Sections (P), Immunoprecipitation (IP)

Background of HTRA2 / PRSS25 antibody

HtrA2 isoform 1 and 2 are thought to play a role in cellular stress response as they are up-regulated following heat shock and endoplasmic reticulum stress. They promote cell death by binding and inactivating inhibitor of apoptosis proteins (IAPs), which contain baculoviral IAP repeat (BIR) binding domains. This causes an increase in caspase activity. Isoform 1 (though not 2) can also proteolytically cleave IAPs. Additionally HtrA2 isoforms can induce cell death via a mechanism dependent on its serine protease activity that is independent of IAP and caspases. Defects in HtrA2 are linked to Parkinson disease 13. The isoforms are localised to the intermembrane space of the mitochondria to be released into the cytoplasm in response to apoptotic stimuli. Isoform 1 is ubiquitous while isoform 2 is expressed predominantly in the kidney, colon and thyroid.

General readings

Savopoulos JW., et al., (2000) Protein Expr. Purif. 19, 227-34.
Gray CW, et al., (2000) Eur. J. Biochem. 267, 5699-710.
Martins LM, et al., (2002) J. Biol. Chem. 277, 439-44.
Van Loo G, et al., (2002) Cell Death Differ. 9, 20-60.
Seong YM, et al., (2004) Protein Expr. Purif. 33, 200-8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HTRA2 / PRSS25 antibody

Western blot analysis of OMI in U937 lysate with Omi antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HTRA2 / PRSS25 antibody

Western blot analysis of OMI in human colon cell lysates with OMI antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HTRA2 / PRSS25 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using HtrA2 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HTRA2 / PRSS25 antibody

Western blot analysis of extracts of various cell lines, using HTRA2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HTRA2 / PRSS25 antibody

Immunohistochemical detection of HtrA2/ Omi on Paraffin Embedded Section of breast cancer with AM20292AF-N HtrA2 antibody. Mouse IgG2a 18-1-83 Azide Free Hu IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

HTRA2 / PRSS25 antibody

HTRA2 / PRSS25 Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

HTRA2 / PRSS25 antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

HTRA2 / PRSS25 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Spleen. Rabbit Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

HTRA2 / PRSS25 Control Peptide

HTRA2 / PRSS25
50 µg / €160.00
  Acris Antibodies GmbH

HTRA2 / PRSS25 Control Peptide

HTRA2 / PRSS25
50 µg / €160.00
  Acris Antibodies GmbH

HTRA2 / PRSS25 antibody

Western blot analysis: The extracts of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HtrA2/Omi antibody (1:1,000). Proteins werevisualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 1B3 Purified Hu, Ms E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

HTRA2 / PRSS25 antibody

Western blot analysis: The extracts of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HtrA2/Omi antibody (1:1,000). Proteins werevisualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 1B3 Purified Hu, Ms E, WB
50 µl / €230.00
  Acris Antibodies GmbH

HTRA2 / PRSS25 antibody

HTRA2 / PRSS25 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

HTRA2 / PRSS25 antibody

Immunofluorescence of monoclonal antibody to HTRA2 on HeLa cell. [antibody concentration 30 ug/ml] Mouse IgG1 4F10 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HTRA2 / PRSS25 antibody

Immunofluorescence of monoclonal antibody to HTRA2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 3G5 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HTRA2 / PRSS25 antibody

Immunofluorescence of monoclonal antibody to HTRA2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 6H8 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HTRA2 / PRSS25 antibody

Western blot detection of HTRA2 in HL-60 cell lysate. Two protein bands of approximate molecular weight of 50 and 38 kDa were detected using HTRA2 monoclonal antibody, clone 196C429 ( Cat # MAB0099 ) at 3 µg/ml dilution. The 50 kDa and 38 kDa bands may represent precursor and mature form of Omi. Mouse IgG1 196C429 Hu P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

HTRA2 / PRSS25 antibody

Western blot analysis of 293T , MCF - 7 , HT - 29 , HL - 60 , HeLa , mouse brain , and rat brain extracts were resolved by SDS - PAGE , transferred to PVDF membrane and probed with HTRA2 monoclonal antibody , clone 1B3 ( 1 : 1000 ) ( Cat # MAB1070 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 1B3 Hu, Ms E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

HTRA2 / PRSS25 antibody

Immunohistochemistry of HTRA2 in human liver tissue with HTRA2 polyclonal antibody ( Cat # PAB13107 ) at 2 µg/ml. Rabbit Hu P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

HTRA2 / PRSS25 antibody

Immunohistochemistry of HTRA2 in human colon tissue with HTRA2 polyclonal antibody ( Cat # PAB13117 ) at 10 µg/ml. Rabbit Hu P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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