HUB1 antibody

Principal name

HUB1 antibody

Alternative names for HUB1 antibody

YNR032C-A, Ubiquitin-like modifier HUB1

SwissProt ID

Q6Q546 (Yeast)

Gene ID

855767 (HUB1)

Available reactivities

Ye (Yeast)

Available hosts


Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of HUB1 antibody

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Hub1 ("Homologous to UBiquitin") may function as a modifier (see figure 2) but its role is unclear because it lacks the double glycine motif characteristic for ubiquitin and ubiquitin-like modifiers. Recently cell polarity factors Sph1 and Hbt1 have been identified as in vivo targets of Hub1 conjugation.
HUB1 has close homologs in other species including humans. The human homologs is called Ubl5.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HUB1 antibody

Figure 1. Immunoblot of Hub1 fusion protein. Anti-Hub1 antibody generated by immunization with recombinant yeast Hub1 was tested by immunoblot against yeast lysates expressing the Hub1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Hub1 using a 1:500 dilution of IgG fraction of Rabbit-anti-Hub1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Hub1 is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov,, personal communication. Rabbit Purified Ye E, WB
0.5 mg / €520.00
  OriGene Technologies GmbH

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