Huntingtin antibody

Principal name

Huntingtin antibody

Alternative names for Huntingtin antibody

Huntington Disease Protein, HD, IT15, HTT

SwissProt ID

P42858 (Human), P42859 (Mouse), P51111 (Rat)

Gene ID

3064 (HTT), 15194 (Htt)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Rb (Rabbit), Can (Canine), Chimp (Chimpanzee), Chk (Chicken)

Available hosts

Mouse, Rabbit, Rat, Goat

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of Huntingtin antibody

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which trans lates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded p olyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq]
Antigen distribution: Nucleus but also cytoplasmic expression in most tissues. Highest levels in neurons and paneth cells in gastrointestinal tract. Distinct granular expression pattern in several glandular epithelia.

General readings

1. Trushina,E. et al. (2004) Mutant huntingtin impairs axonal trafficking in mammalian neurons in vivo and in vitro. Mol. Cell. Biol. 24 (18), 8195-
8209.
2. Lee,W.C., Yoshihara,M. and Littleton,J.T. (2004) Cytoplasmic aggregates trap polyglutamine-containing proteins and block axonal transport in
a Drosophila model of Huntington's disease. Proc. Natl. Acad. Sci. U.S.A. 101 (9), 3224-3229.
3. Zainelli,G.M., Ross,C.A., Troncoso,J.C., Fitzgerald,J.K. and Muma,N.A. (2004) Calmodulin regulates transglutaminase 2 cross-linking of huntingtin. J. Neurosci. 24 (8), 1954-1961.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Huntingtin antibody

Immunoperoxidase of monoclonal antibody to HD on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml] Mouse IgG2a 3F1 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Huntingtin antibody

Immunofluorescent: Huntingtin Antibody [NBP1-03377] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to internal part of Huntingtin (Huntington disease protein homolog, Htt, HD protein homolog): whole serumat 1: 500 dilution. DAPI counter stained appearing in blue. Rabbit Serum Ms, Rt C, ICC/IF, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Huntingtin antibody

Western blot analysis of Huntingtin expression in HEK293 cells. Mouse IgG H.F5 Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Huntingtin antibody

Western Blot: Huntingtin Antibody (1A771) [TA336451] - Analysis of Htt in four different lymphoblast HD cell lines extracts each expressing ~65 (mutant) and ~20 (normal) CAG repeats. Lanes 1-4. A, HTT mAb clone 1A771 . B. An Htt antibody recognizing both Mouse IgG 1A771 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Huntingtin (85-200) antibody

Huntingtin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
20 µl / €50.00
  SICGEN

Huntingtin (85-200) antibody

Huntingtin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
0.6 mg / €320.00
  SICGEN

Huntingtin (1247-1646) antibody

Immunohistochemistry on paraffin sections: Human hypothalamus, stained with AM26742PU-N. Mouse IgG2b 3HU-4E6 Purified Hu, Ms P
0.1 mg / €390.00
  Acris Antibodies GmbH

Huntingtin antibody

Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse 3F1 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Huntingtin pSer421 antibody

Brain cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Huntingtin (C-term) antibody

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Huntingtin control peptide

Huntingtin Purified
50 µg / €190.00
  Acris Antibodies GmbH

Huntingtin control peptide

Huntingtin Purified
50 µg / €190.00
  Acris Antibodies GmbH

Huntingtin pSer421 antibody

Figure 2. Immunohistochemistry. 
Affinity Purified anti-Huntingtin pS421 antibody was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum. 
Positive cytoplasmic staining is observed in neurons. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA. Rabbit Aff - Purified Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Huntingtin antibody

Total protein extract of normal human cerebral cortex separated as a strip on a 3-12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with SM1660. Mouse IgG2a HDA3E10 Purified Hu, Ms, Rb C, IP, WB
0.1 mg / €540.00
  Acris Antibodies GmbH

Huntingtin antibody

Total protein extract of normal human cerebral cortex separated as a strip on a 3-
12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with SM1661. Mouse IgG1 HDB4E10 Purified Hu, Ms, Rb C, IP, WB
0.1 mg / €540.00
  Acris Antibodies GmbH

Huntingtin antibody

Total protein extract of normal human cerebral cortex separated as a strip on a 3- 12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with SM1662. Mouse IgG1 HDC8A4 Purified Hu, Ms, Rb C, IP, WB
0.1 mg / €540.00
  Acris Antibodies GmbH

Huntingtin antibody

Huntingtin Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Huntingtin antibody

Detection limit for recombinant GST tagged HD is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 2E10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Huntingtin antibody

Detection limit for recombinant GST tagged HD is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 4G6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Huntingtin antibody

Detection limit for recombinant GST tagged HD is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 1H6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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