IAP1 / BIRC3 antibody

Principal name

IAP1 / BIRC3 antibody

Alternative names for IAP1 / BIRC3 antibody

API2, MIHC, RNF49, C-IAP2, cIAP2, IAP homolog C, Apoptosis inhibitor 2, HIAP-1, Baculoviral IAP repeat-containing protein 3, Inhibitor of apoptosis protein 1, TNFR2-TRAF-signaling complex protein 1, RING finger protein 49

SwissProt ID

A1E2V0 (Canfa), O08863 (Mouse), Q13489 (Human)

Gene ID

330 (BIRC3), 329 (BIRC2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of IAP1 / BIRC3 antibody

cIAP2 (API2, BIRC3, HIAP2, MIHC) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including cIAP2, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination. Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. The IAPs, including cIAP2, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage (see Vischioni et al. 2005 for a comprehensive study). cIAP2-MALT (API2-MALT1) fusion proteins have been implicated in the molecular pathology of mucosa associated lymphoid tissue (MALT) lymphoma (reviewed in Hosokawa, 2005). AP12-MALT fusion proteins are found in a subset of patients with MALT lymphoma. They are generated by chromosomal translocations whereby the N-terminal portion of AP12 (including the BIR domains) is linked to the C-terminal portion of MALT1 (containing at least an immunoglobulin-like domain and a caspase domain). Several variants of the API2-MALT1 fusion proteins can occur in MALT1 lymphoma patients depending on the chromosomal breakpoints. It is thought that AP12-MALT1 can enhance the activation of NK-kB signalling, which may be relevant to the pathology of MALT lymphomas. This antibody recognizes cIAP2, human cIAP2 is a 604 amino acid protein. This antibody should also recognize AP12-MALT1 fusion proteins assuming that they contain the cIAP2 peptide sequence (QDFSALMRSSYHCAMNNENAR) used for immunogen. The AP12-MALT1 fusion proteins is listed as an 1140 amino acid protein, GenBank no. AAD46161.1. However users should keep in mind that the actual amino acid length of AP12-MALT1 fusion proteins may vary depending on the chromosomal breakpoints.

General readings

1. Zheng C, Kabaleeswaran V, Wang Y, Cheng G, Wu H,
Crystal structures of the TRAF2: cIAP2 and the TRAF1: TRAF2: cIAP2 complexes: affinity, specificity, and regulation. Molecular cell 2010 Apr 38 (1): 101-13.
PMID: 20385093

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IAP1 / BIRC3 antibody

Western Blot: cIAP2 Antibody [TA336748] - HeLa whole cell lysate. Rabbit Purified Hu C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IAP1 / BIRC3 (314-508) antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

IAP1 / BIRC3 (109-118) antibody

(0.1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IAP1 / BIRC3 antibody

Western blot detection of c-IAP2 in Ramos,COS7,Raji and Daudi cell lysates using c-IAP2 mouse mAb (1:1000 diluted).Predicted band size: 68KDa,Observed band size:72KDa. Mouse IgG1 1F12-E5-G12 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IAP1 / BIRC3 antibody

ELISA: cIAP2 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

IAP1 / BIRC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC3 (RC207764, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC3. Mouse IgG1 OTI1E3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IAP1 / BIRC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC3 (RC207764, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC3. Mouse IgG1 OTI1A9 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IAP1 / BIRC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC3 (RC207764, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC3. Mouse IgG1 OTI1F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IAP1 / BIRC3 antibody

Anti-BIRC3 mouse monoclonal antibody (TA800031) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BIRC3(RC207764). Mouse IgG1 OTI1D12 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IAP1 / BIRC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC3 (RC207764, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC3. Mouse IgG1 OTI2G7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IAP1 / BIRC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC3 (RC207764, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC3. Mouse IgG1 OTI2B7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IAP1 / BIRC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC3 (RC207764, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC3. Mouse IgG1 OTI2B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IAP1 / BIRC3 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BIRC3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG1 OTI3G4 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

IAP1 / BIRC3 control peptide

IAP1 / BIRC3 Purified
50 µg / €190.00
  Acris Antibodies GmbH

IAP1 / BIRC3 antibody

IAP1 / BIRC3 Goat Aff - Purified Hu, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

IAP1 / BIRC3 antibody

Western Blot analysis of BIRC3 expression in transfected 293T cell line (H00000330-T01) by BIRC3 MaxPab polyclonal antibody.

Lane 1: BIRC3 transfected lysate(66.44 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

IAP1 / BIRC3 antibody

IAP1 / BIRC3 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

IAP1 / BIRC3 antibody

IAP1 / BIRC3 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

IAP1 / BIRC3 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between BIRC3 and CASP9 HeLa cells were stained with anti-BIRC3 rabbit purified polyclonal 1:1200 and anti-CASP9 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

IAP1 / BIRC3 antibody

Immunohistochemical analysis of BIRC3 in paraffin-embedded formalin-fixed human mammary gland ( female breast ) using BIRC3 polyclonal antibody ( Cat # PAB0253 ) at 1:2000.
A, normal breast tissue.
B, ductal carcinoma in situ ( DCIS ).
C. invasive neoplasia.
BIRC3 expressed increased sucessively with tumor progression.
Hematoxylin-eosin counterstain. Rabbit Hu C, IP, P, WB
50 µl / €390.00
  Abnova Taiwan Corp.

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