IAP4 / BIRC5 antibody

Principal name

IAP4 / BIRC5 antibody

Alternative names for IAP4 / BIRC5 antibody

Survivin, Apoptosis inhibitor 4, API4, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor survivin

SwissProt ID

O15392 (Human), O70201 (Mouse), Q6J1J1 (Bovin), Q8I009 (Canfa), Q9GLN5 (Pig), Q9JHY7 (Rat)

Gene ID

332 (BIRC5), 11799 (Birc5), 64041

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Fe (Feline)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F), Luminex (LMNX)

Background of IAP4 / BIRC5 antibody

Survivin is a novel member of a group of proteins belonging to inhibitors of apoptosis (IAP). Survivin is expressed in cancer cells but is undetectable in normal, differentiated adult tissues. Survivin is expressed during embryonal development but lacks expression in terminally differentiated adult tissues. It has been shown that it is expressed in tumor cells from different cell lineages. It can inhibit apoptosis that is induced by a variety of factors, and plays important roles during mitosis and cytokinesis of cell. However, the mechanism of regulation of the survivin expression still remains unclear. Recent research suggest that surviving is implicated in regulation of cell division and control of apoptosis. In certain lung cancer cells production of nitric oxide down regulates the expression of surviving and induce apoptosis (1). Recently, several apoptosis inhibitors related genes have been found in various species, including human. These genes code for proteins that contain 1-3 Cys/His baculovirus IAP repeats plus a c-terminal RING finger. These structural motifs are thought to inhibit various steps in self induce apoptotic process. The overexpression of surviving is also implicated in resistance to chemotherapy of certain tumor cells. Thus, Survivin is associated with cytoprotectionand preservation of cell shape (2) , and its expressionin malignant cells may facilitate evasion from apoptosis andpromote aberrant mitotic progression, thus exacerbating aneuploidy.
Survivin is a 16 kDa protein with amino acids. The expression of Survivin in prenatal tissue is turned off and not found in non-neoplastic adult human and other species tissues. The expression of this important protein is turned on again in transformed cells and in the most common cancers of lung, colon, pancreas , breast, and proastate in vivo. The presence of survivin is believed to regulate and control the cell death and cell division by controlling a check point involved in cytokinesis and suppressing apoptosis. The surviving also inhibits Fas-mediated apoptosis in variouis immune cells (2). In lung cancinoma cells, surviving is a phosphorlyated by protein kinase B (3). One of the important step in survivin function is identified recently which require phosphorylation of survivin on Thr34 by the mitotic kinase p34cdc2-cyclin B1 (4) which inhibit p53 induced apoptosis (2). Phosphorylation of surviving by PKB has been shown to arrest the cells in G2/M phase (3). It was foundthat phosphorylation on Thr34 is required to maintain survivinexpression/stability in cancer cells and that ablation of p34cdc2 kinase in mitotically arrested cells results in loss of survivinlevels and dramatic enhancement of chemotherapy-induced anticanceractivity in vivo.

General readings

1. Blanc-Brude, O. P.; Yu, J.; Simosa, H.; Conte, M. S.; Sessa, W. C.; Altieri, D. C. : Inhibitor of apoptosis protein survivin regulates vascular injury. Nature Med. 8: 987-994, 2002.
2. Ambrosini, G.; Adida, C.; Altieri, D. C. : A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nature Med. 3: 917-921, 1997.
3. Li, F.; Ambrosini, G.; Chu, E. Y.; Plescia, J.; Tognin, S.; Marchisio, P. C.; Altieri, D. C. : Control of apoptosis and
mitotic spindle checkpoint by survivin. Nature 396: 580-584, 1998.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IAP4 / BIRC5 antibody

Detection of survivin in HeLa whole cell lysate (30 ug) Rabbit IgG Purified Can, Fe, Hu, Ms, Rt WB
50 µl / €325.00
  OriGene Technologies, Inc.

IAP4 / BIRC5 antibody

Western blot analysis of extracts of various cell lines, using BIRC5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

IAP4 / BIRC5 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BIRC5  mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA501245, Dilution 1:50) Mouse IgG1 OTI2D9 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

IAP4 / BIRC5 antibody

Anti-BIRC5 mouse monoclonal antibody (TA502234) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BIRC5(RC205935). Mouse IgG1 OTI2D3 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IAP4 / BIRC5 antibody

Immunofluorescence of monoclonal antibody to BIRC5 on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG2a 5B10 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

IAP4 / BIRC5 (C-term) antibody

AM09114PU Survivin antibody staining of Human Stomach. Rabbit IgG SP79 Purified Hu P
0.5 ml / €520.00
  Acris Antibodies GmbH

IAP4 / BIRC5 (C-term) antibody

AM09114PU Survivin antibody staining of Human Stomach. Rabbit IgG SP79 Purified Hu P
1 ml / €750.00
  Acris Antibodies GmbH

IAP4 / BIRC5 (C-term) antibody

AM09114PU Survivin antibody staining of Human Stomach. Rabbit IgG SP79 Purified Hu P
0.1 ml / €300.00
  Acris Antibodies GmbH

IAP4 / BIRC5 antibody

Human stomach stained with Anti-Survivin antibody. Rabbit Purified Hu, Ms P
0.5 ml / €360.00
  Acris Antibodies GmbH

IAP4 / BIRC5 antibody

Human stomach stained with Anti-Survivin antibody. Rabbit Purified Hu, Ms P
1 ml / €450.00
  Acris Antibodies GmbH

IAP4 / BIRC5 antibody

Human stomach stained with Anti-Survivin antibody. Rabbit Purified Hu, Ms P
0.1 ml / €180.00
  Acris Antibodies GmbH

IAP4 / BIRC5 antibody

Survivin detected in rat aorta smooth muscle cell lysate Mouse IgG2a 60.11 Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IAP4 / BIRC5 antibody

Lane 1: Phosphorylated Survivin protein and Lane 2: Non-phosphorylated Survivin protein. Rabbit IgG Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

IAP4 / BIRC5 antibody

Staining of human lung, cancer Mouse IgG1 32.1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IAP4 / BIRC5 antibody

Detection of Survivin in HeLa whole cell extract Mouse IgG2a 60.11 Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

IAP4 / BIRC5 antibody

Detection of Survivin [Ser20] in phosphorylated recombinant protein (+), but not in unphosphorylated protein (-) Rabbit IgG Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

IAP4 / BIRC5 (C-term) antibody

TA302956 (0.01µg/ml) staining of Molt-4 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IAP4 / BIRC5 (N-term) antibody

Western blot analysis of Survivin in MOLT4 cell lysate with Survivin antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IAP4 / BIRC5 (C-term) antibody

Western blot analysis of survivin in MOLT4 cell lysate in the absence (A) or presence (B) of blocking peptide with survivin antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IAP4 / BIRC5 (C-term) antibody

Western blot analysis of survivin in mouse spleen tissue lysate with survivin antibody at 1 ug/ml. Rabbit IgG Aff - Purified Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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