ID3 / BHLHB25 antibody

Principal name

ID3 / BHLHB25 antibody

Alternative names for ID3 / BHLHB25 antibody

1R21, Helix-loop-helix protein HEIR-1, HEIR1, Inhibitor of DNA binding 3, DNA-binding protein inhibitor ID-3

SwissProt ID

P41133 (Mouse), P41138 (Rat), Q02535 (Human), Q5E981 (Bovin), Q712G9 (Canfa)

Gene ID

3399 (ID3), 15903 (Id3), 25585

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Luminex (LMNX)

Background of ID3 / BHLHB25 antibody

Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

General readings

Tsuchiya,T., (2005) Cancer Sci. 96 (11), 784-790

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ID3 / BHLHB25 antibody

  ID3 mouse monoclonal antibody, clone OTI10D3 (formerly 10D3)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500791) Mouse IgG1 OTI10D3 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ID3 / BHLHB25 antibody

  ID3 mouse monoclonal antibody, clone OTI8B3 (formerly 8B3)  
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500713) Mouse IgG1 OTI8B3 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ID3 / BHLHB25 antibody

  ID3 mouse monoclonal antibody, clone OTI5C7 (formerly 5C7)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500714) Mouse IgG2a OTI5C7 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ID3 / BHLHB25 antibody

  ID3 mouse monoclonal antibody, clone OTI4D1 (formerly 4D1)  
Anti-ID3 mouse monoclonal antibody (TA500715) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ID3(RC200583). Mouse IgG1 OTI4D1 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ID3 / BHLHB25 antibody

  ID3 mouse monoclonal antibody, clone OTI15F4 (formerly 15F4)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500716) Mouse IgG1 OTI15F4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ID3 / BHLHB25 antibody

  anti-ID3 mouse monoclonal antibody, clone OTI15E3 (formerly 15E3)  
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500757, Dilution 1:50) Mouse IgG1 OTI15E3 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ID3 / BHLHB25 antibody

  ID3 mouse monoclonal antibody, clone OTI12C7 (formerly 12C7)  
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ID3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500790) Mouse IgG1 OTI12C7 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ID3 / BHLHB25 antibody

  anti-ID3 mouse monoclonal antibody, clone OTI7B6 (formerly 7B6)  
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ID3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500811, Dilution 1:50) Mouse IgG1 OTI7B6 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ID3 / BHLHB25 antibody

  ELISA and Luminex validated ID3 mouse monoclonal antibody (clone 15F, clone OTI15F4 (formerly 15F4)  
ID3 ELISA with 15F4 Capture (TA600104) and 5C7 Detection (TA700104) Antibodies. Substrate used: Recombinant Human ID3 (TP300583) Mouse IgG1 OTI15F4 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

ID3 / BHLHB25 antibody

  ELISA and Luminex validated biotinylated ID3 mouse monoclonal antibody (clone 4D1), clone OTI4D1 (formerly 4D1)  
ID3 ELISA with 15F4 Capture (TA600104) and 4D1 Detection (TA700103) Antibodies. Substrate used: Recombinant Human ID3 (TP300583) Mouse IgG1 OTI4D1 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

ID3 / BHLHB25 antibody

  ELISA and Luminex validated biotinylated ID3 mouse monoclonal antibody (clone 5C7), clone OTI5C7 (formerly 5C7)  
ID3 ELISA with 15F4 Capture (TA600104) and 5C7 Detection (TA700104) Antibodies. Substrate used: Recombinant Human ID3 (TP300583) Mouse IgG1 OTI5C7 Purified Hu E, LMNX
50 µg / €345.00
  OriGene Technologies, Inc.

ID3 / BHLHB25 (C-term) antibody

Human Lung (formalin-fixed, paraffin-embedded) stained with ID3 antibody AP22732PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu P
0.25 ml / €470.00
  Acris Antibodies GmbH

ID3 / BHLHB25 antibody

  Rabbit Polyclonal Anti-ID3 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (ID3 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ID3 / BHLHB25 antibody

  Rabbit Polyclonal anti-ID3 Antibody  
Human Breast Carcinoma stained with anti-ID3 antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ID3 / BHLHB25 antibody

  Rabbit Polyclonal ID3 Antibody  
ID3 / BHLHB25 Rabbit IgG Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

ID3 / BHLHB25 antibody

  ELISA and Luminex validated ID3 mouse monoclonal antibody (clone 15E, clone OTI15E3 (formerly 15E3)  
ID3 ELISA with 15E3 Capture (TA600103) and 5C7 Detection (TA700104) Antibodies. Substrate used: Recombinant Human ID3 (TP300583) Mouse IgG1 OTI15E3 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

ID3 - human gene knockout kit via CRISPR

ID3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ID3 - human gene knockout kit via CRISPR

ID3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ID3 - human gene knockout kit via CRISPR

ID3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ID3 - human gene knockout kit via CRISPR

ID3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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