ID4 / BHLHB27 antibody

Principal name

ID4 / BHLHB27 antibody

Alternative names for ID4 / BHLHB27 antibody

DNA-binding protein inhibitor ID-4, Inhibitor of DNA binding 4, Class B basic helix-loop-helix protein 27

SwissProt ID

P41139 (Mouse), P47928 (Human), Q06AV5 (Pig)

Gene ID

3400 (ID4), 15904 (Id4)

Available reactivities

Hu (Human), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Bov (Bovine), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of ID4 / BHLHB27 antibody

Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins.Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Fontemaggi, G., et al. Nat. Struct. Mol. Biol. 16(10):1086-1093(2009)
Uhm, K.O., et al. J. Korean Med. Sci. 24(3):493-497(2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ID4 / BHLHB27 antibody

  Rabbit polyclonal anti-ID4 antibody  
Western blot analysis of extracts from HepG2 cells, using ID4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ID4 / BHLHB27 antibody

  Anti-ID4 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human liver cancer tissue. Recommended dilution: 1/20-100  The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using ID4 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ID4 / BHLHB27 antibody

  Anti-ID4 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human esophagus and liver cancer tissue. Recommended dilution: 1/20-100  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus and liver cancer tissue using ID4 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ID4 / BHLHB27 antibody

  Rabbit Polyclonal Anti-ID4 Antibody  
Lanes : Lane 1: 10ug MDA-MB231 lysateLane 2: 10ug MCF7 lysate; Primary Antibody Dilution : 1:1000; Secondary Antibody : Anti-rabbit-HRP; Secondary Antibody Dilution : 1:10,000; Gene Name : ID4; Submitted by : Maria Teresita Branham. Facultad de Cs Mè??dic Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ID4 / BHLHB27 antibody

  Rabbit Polyclonal Anti-ID4 Antibody  
WB Suggested Anti-ID4 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Can, Hu, Ms, Por, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ID4 / BHLHB27 antibody

  Rabbit Polyclonal Anti-ID4 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (ID4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ID4 / BHLHB27 antibody

  ID4 mouse monoclonal antibody, clone OTI2G10 (formerly 2G10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID4 (RC204170, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID4. Mouse IgG1 OTI2G10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ID4 / BHLHB27 antibody

  ID4 mouse monoclonal antibody, clone OTI3H1 (formerly 3H1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID4 (RC204170, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID4. Mouse IgG1 OTI3H1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ID4 / BHLHB27 (N-term) antibody

Western blot analysis with extracts from HepG2 cells using ID4 / BHLHB27 antibody (Cat.-No. AP01280PU-N). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ID4 / BHLHB27 (N-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded bladder carcinoma reated with ID4 Antibody (N-term) Cat.-No AP52145PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ID4 / BHLHB27 antibody

Western blot analysis of extracts from HepG2 cells, using ID4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

ID4 / BHLHB27 antibody

Western blot analysis of extracts from HepG2 cells, using ID4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

ID4 - human gene knockout kit via CRISPR

ID4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ID4 - human gene knockout kit via CRISPR

ID4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ID4 - human gene knockout kit via CRISPR

ID4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Id4 - mouse gene knockout kit via CRISPR

Id4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Id4 - mouse gene knockout kit via CRISPR

Id4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Id4 - mouse gene knockout kit via CRISPR

Id4 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Id4 - mouse gene knockout kit via CRISPR

Id4 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ID4 / BHLHB27

  Recombinant protein of human inhibitor of DNA binding 4, dominant negative helix-loop-helix protein (ID4)  
ID4 / BHLHB27 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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