IDO2 / INDOL1 antibody

Principal name

IDO2 / INDOL1 antibody

Alternative names for IDO2 / INDOL1 antibody

Indoleamine 2,3-dioxygenase-like protein 1,Indoleamine 2,3-dioxygenase-like protein 1, Indoleamine-pyrrole 2,3-dioxygenase-like protein 1

SwissProt ID

Q6ZQW0 (Human), Q8R0V5 (Mouse)

Gene ID

169355 (IDO2), 209176

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Eq (Equine), Por (Porcine), Rb (Rabbit), Rt (Rat)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP)

Background of IDO2 / INDOL1 antibody

IDO2(Indoleamine 2,3-dioxygenase 2), also called INDOLEAMINE 2,3-DIOXYGENASE-LIKE 1 or INDOL1, is an enzyme encoded by the INDOL1 gene which metabolizes tryptophan in the kynurenine pathway. By genomic sequence analysis, the INDOL1 gene is mapped on chromosome 8p12 just downstream of the INDO gene. And its exact cytogenetic location is 8p11.21. By database analysis using INDO as probe, followed by RT-PCR of total RNA from various tissues, IDO2 is cloned by human and mouse INDOL1.INDOL1 catabolizes tryptophan as determined by Kyn production, but unlike INDO, is inhibited by D-1-methyl-tryptophan (D-1MT) but not the L-1MT stereoisomer.The Gene Structure of the INDOL1 has 11 exons and spans 74 kb.

General readings

1. Metz R, Duhadaway JB, Kamasani U, Laury-Kleintop L, Muller AJ, Prendergast GC.
Novel tryptophan catabolic enzyme IDO2 is the preferred biochemical target of the antitumor indoleamine 2,3-dioxygenase inhibitory compound D-1-methyl-tryptophan.
Cancer research 2007 Aug 67 (15): 7082-7.
PMID: 17671174

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IDO2 / INDOL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (RC223337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2. Mouse IgG1 OTI1A4 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with IDO2 / INDOL1 Antibody (C-term) Cat.-No AP52135PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

IDO2 / INDOL1 (C-term) antibody

(0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IDO2 / INDOL1 antibody

WB of Anti-IDO2 Antibody. Left Figure: Lane 1: HEK293 expressing human IDO-1 V5 tag. Lane 2: HEK293 expressing human IDO-2 V5 tag. Primary antibody: V5 antibody. Secondary antibody: rabbit secondary antibody HRP. Right Figure: Lane 1: HEK293 expressing human IDO-1 V5 tag. Lane 2: HEK293 expressing human IDO-2 V5 tag. Primary antibody: IDO-2 antibody at 1:2000-10,000. Secondary antibody: rabbit secondary antibody HRP. Rabbit IgG Purified Hu, Ms E, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IDO2 / INDOL1 antibody

Western Blot of Mouse Anti-IDO2 antibody. Lane 1: HEK293 no transgene cell extracts. Lane 2: mouse IDO-2 transgene expressed in 293HEK. Lane 3: mouse IDO-1 transgene expressed in 293HEK. Primary antibody: IDO2 monoclonal Antibody. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 44.4 kDa, ~60 kDa for IDO 2. Other band(s): IDO2 splice variants and isoforms. Mouse IgG 1HC Purified Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

IDO2 / INDOL1 antibody

WB Suggested Anti-IDO2 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Brain Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

IDO2 / INDOL1 antibody

Immunofluorescent staining of HeLa cells using anti-IDO2 mouse monoclonal antibody (TA506646). Mouse IgG1 OTIB9 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-IDO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806160) Mouse IgG1 OTI11B2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (RC223337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2. Mouse IgG1 OTI6G3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (RC223337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2. Mouse IgG1 OTI4B3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (RC223337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2. Mouse IgG2b OTI12G4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (RC223337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2. Mouse IgG1 OTI8B5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (RC223337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2. Mouse IgG2a OTI6C6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (RC223337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2. Mouse IgG2b OTI4H4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (RC223337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2. Mouse IgG1 OTI2D11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-IDO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806648) Mouse IgG2a OTI19F8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (RC223337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2. Mouse IgG2b OTI2E10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

IDO2 / INDOL1 antibody

Western Blot of Mouse Anti-IDO2 antibody. Lane 1: HEK293 no transgene cell extracts. Lane 2: mouse IDO-2 transgene expressed in 293HEK. Lane 3: mouse IDO-1 transgene expressed in 293HEK. Primary antibody: IDO2 monoclonal Antibody. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 44.4 kDa, ~60 kDa for IDO 2. Other band(s): IDO2 splice variants and isoforms. Mouse IgG 1HC Purified Ms E, ICC/IF, WB
25 µl / €190.00
  Acris Antibodies GmbH

IDO2 / INDOL1 antibody

IDO2 / INDOL1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

IDO2 / INDOL1 antibody

IDO2 / INDOL1 Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

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